Storing PK\LDH without any sulfate ?

Jean-Manuel Henry ez041797 at bullwinkle.ucdavis.edu
Sat Oct 15 01:39:10 EST 1994


I am wondering if anyone knows a method of stabilizing PK\LDH in solution. 
The enzymes are stored for the long term in 70% ammonium sulfate but are
desalted for use in an assay for ATP sulfurylase. Pk\LDH activity 
decreases rapidly, and the desalted solutions are minimally active after 
~4 days. So any ideas on how to preserve the enzymes would be greatly 
appreciated.




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