VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY

Bruce James Ella 092ellbj at chiron.wits.ac.za
Mon Oct 17 03:28:00 EST 1994


I am fractionating the low molecular weight proteins in serum on a G50 
Sephadex column. After which I need to freeze dry the 6 fractions but remove 
the salt concentration such that the sample can be added to cell medium. 
I figured that elution of the samples from the column with a volatile 
buffer, eg ammonium formate or carbonate, would be the answer. Can any one 
help?



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