NaAc precipitation

anon anon
Mon Oct 17 19:15:55 EST 1994


In article <9410121941.AA61115 at acs5.acs.ucalgary.ca>,
sfraser at ACS.UCALGARY.CA ("Sherri Fraser") wrote:

> We've been finding recently that when we precipitate DNA with
> sodium acetate (pH 7-8) with ethanol that when we add the
> ethanol, the sodium acetate (we think) precipitates out and
> leaves us with a huge pellet.

 Is the pellet a problem? If you do 70% ethanol washes you should be able
to remove the salt, also we have found that ammonium acetate (same conc.)
is easier to remove with washes, and this especially helps for preparing
DNA for double stranded sequencing.



More information about the Methods mailing list