HELP!!! Electroporation and PEG
David L. Haviland, Ph.D.
HAVILAND at KIDS.WUSTL.EDU
Mon Oct 17 17:19:57 EST 1994
In <lopez.8.000B45BF at medusa.bioc.aecom.yu.edu>
lopez at medusa.bioc.aecom.yu.edu writes:
>I recently read somewhere a protocol to precipitate PEG-DNA ligations in
>order to remove the PEG . According t the article PEG reduces the
>efficiency of electroporation. My problem is that can not find that
>article, I cant remember if I saw it on Biotecniques , Focus or any of the
>other "mini" journals. I will appreciate if someone can tell me where to
>find this article. Thank you very much in advance.
> See you on the dark side of the Moon.
Hmmmm.... never heard of this: What I have read in Biotechniques, if
memory serves, is to dry butanol your ligations (somewhat akin to
Sawadaggo et al, for preciping Oligos) down. Spin in a microfuge and then
wash the pellet with 70-80% EtOH. Rehydrate in pure (pure as can be) water
and then electroporate - I'll see if I can dig up the reference unless
someone else can beat me to it...
Hope this helps,
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