Help! DNA insoluble in water
nxu at uoguelph.ca
Mon Oct 17 13:42:09 EST 1994
It drives me crazy that my DNA won't dissolve in water! I put 60
ug plasmid DNA (pUC19 + 1.5 kb insert) in 60 ul water and digested it
with Nco I, phenol extracted, precipitated with 2 vol. ethanol, washed
and dried in a Speed-Vac (5 Min.), and I cannot get it dissolved in water
again. I also tried phenol/chloroform extraction and no extraction,
drying at 65C or at room temperature. Nothing has worked repreducibly.
What I did wrong? What can I do to make this DNA dissolve in water?
P.S. I tried precipitating the same DNA without digestion. It dissolved
in water perfectly no matter how it was dried or precipitated.
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