ANCIENT DNA

[Tracy Aquilla]

In Article <cstroy-181094104436 at gen023.gen.tcd.ie>, cstroy at mail.tcd.ie
(Chris Troy) wrote:
>I have received some interesting messages about autoclaving solutions and I
>have been advised by some people to UV my solutions before use. As the
>general term: " UV solutions", is not that informative, could anyone advise
>me on general procedures for the UV irradiation of PCR buffers etc. Can you
>simply irradiate  through the eppendorf tubes or do you have to leave them
>open??????

You can irradiate through the tubes. I use a StrataLinker, but you can also
leave them on top of your UV-transilluminator for five to ten minutes (with
the UV lamp ON, of course). Good luck.
    Tracy