Artifacts in silver staining

Frederick Garbrecht FRED at bmt.mcw.edu
Tue Oct 18 10:17:59 EST 1994


> To:            methods-and-reagents at net.bio.net
> From:          092ellbj at chiron.wits.ac.za (Bruce James Ella)
> Subject:       Artifacts in silver staining
> Date:          Tue, 18 Oct 1994 10:47:31 GMT

> I have been experiencing an artifact problem in my SDS-PAGE silver staining 
> gels in analysis if proteins. The artifact band appears always at approx. 10 
> 000 mw position. I use ultra-pure acrylamide (MERCK), good water (MILLI-Q)
> and analytical grade glycine. CAN ANYONE HELP!!!!!!
> 
> 
What are the proteins you are running on SDS-PAGE?  What makes you 
think that your "artifactual" band is an artifact?  More info would 
help us help you.

Frederick C. Garbrecht
fred at bmt.mcw.edu
Bone Marrow Transplant Program
Medical College of Wisconsin
phone 414 257 5053
fax   414 257 7994



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