exonuclease I

Parke K. Flick ch994 at cleveland.Freenet.Edu
Wed Oct 19 09:08:19 EST 1994

There is nothing new about Exonuclease I.  The enzyme was first
described by Lehman and Nussbaum in 1964 and it has been sold by
USB for years.  It is not recommended for blunting DNA--does not
leave a perfectly blunt end.  Rather it is good for things like
getting rid of oligo primers in PCR reactions, etc.  It is a
key component of USB's PCR Sequencing Kit.
P. Flick
Parke K. Flick, Ph.D.  ch994 at cleveland.freenet.edu
USB/Amersham Life Sciences, Inc.
Cleveland, OH  44128  Ph: 216-464-9277; FAX: 216-360-0975

More information about the Methods mailing list