NaAc precipitation

Michael Cooley szcooley at dale.ucdavis.edu
Thu Oct 20 11:28:54 EST 1994


Sherri Fraser (sfraser at ACS.UCALGARY.CA) wrote:
: We've been finding recently that when we precipitate DNA with
: sodium acetate (pH 7-8) with ethanol that when we add the
: ethanol, the sodium acetate (we think) precipitates out and
: leaves us with a huge pellet.  It doesn't seem to be a problem if
: the sodium acetate solution is new. We've noticed this with at
: least three different sodium acetate solutions we've made up by
: two different people.  I've also used these solutions for
: cleaning up DNA after alkaline lysis DNA preps with no problems.  It only appears when
: we do sequencing or when we clean up after dephosphorylation.
: 	Can anybody give suggestions?
: Thanks in advance
: -----
: Sherri Fraser
: Dept. Biol. Sci.
: Univ. of Calgary
: Canada

I never get a precip from sodium acetate. The only thing that I notice is 
that we adjust the pH of the 3M stock to 5.2 with acetic acid. I don't 
know if this will make a difference.


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