Adding membrane signal to cytoplasmic protein

ORSULIC at UNCVX1.OIT.UNC.EDU ORSULIC at UNCVX1.OIT.UNC.EDU
Thu Oct 20 17:29:43 EST 1994





I am working on structure/function analysis of Drosophila Armadillo protein.
The protein is about 105 kb, does not have a signal or transmembrane sequence,
and is mostly cytoplasmic. 

I would like to generate the form of the protein that would be tethered to the
membrane. Any suggestions, protocols or references of successful addition of a
myristilation or membrane signal to other proteins would be appreciated. 


Sandra Orsulic
University of North Carolina

orsulic at uncvx1.oit.unc.edu



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