Question on generating nicked circular DNA using EthBr and EcoRI

Rock Pulak rapulak at
Fri Oct 21 15:49:18 EST 1994

I'm asking this question for a friend.

Does anyone have a protocol for generating nicked circular DNA from
supercoiled DNA by using ethidium bromide and Eco RI (or any other 
restriction enzyme)?  Either a detailed protocol or a reference would
be greatly appreciated.  

Please email to either:

Rock Pulak at rapulak at

Paul Marrione   at   marrione at

More information about the Methods mailing list