Question on generating nicked circular DNA using EthBr and EcoRI
rapulak at macc.wisc.edu
Fri Oct 21 15:49:18 EST 1994
I'm asking this question for a friend.
Does anyone have a protocol for generating nicked circular DNA from
supercoiled DNA by using ethidium bromide and Eco RI (or any other
restriction enzyme)? Either a detailed protocol or a reference would
be greatly appreciated.
Please email to either:
Rock Pulak at rapulak at macc.wisc.edu
Paul Marrione at marrione at biochem.wisc.edu
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