Plasmid copy number control

bchs1b BENEDIK at UH.EDU
Fri Oct 21 11:57:41 EST 1994


Does anyone have a slick way to control copy number of pBR/pUC
plasmids? 
I don't think I can use polA, because my cells are recA and I
understand 
recA polA mutants are not viable.

Thanks.


Jim Hartley
Life Technologies, Inc.


PolA doesn't really control replication, it just stops it. What
precisely
are you wanting to do, maybe I/others can make some suitable
suggestions but
it isn't clear what you are looking for.



MICHAEL BENEDIK                                          BENEDIK at UH.EDU
BIOCHEMICAL SCIENCES
UNIVERSITY OF HOUSTON
HOUSTON, TX 77204-5934



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