ammonium sulfate precipitation of proteins

Flip Hoedemaeker sbtnfh at
Fri Oct 21 06:25:06 EST 1994

In article <Cxzs46.HHr at>, bipin dalmia <dalmiabk at> writes:
>centrifugation at 20,000 g for 20 minutes and NO PELLET!!! can someone
>explain what happened and how i can recover my protein?

I think keeping your mixture on ice for at least an hour will improve things.
Maybe you should also increase your G-force!


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