ammonium sulfate precipitation of proteins

Flip Hoedemaeker sbtnfh at rulsfb.LeidenUniv.nl
Fri Oct 21 06:25:06 EST 1994


In article <Cxzs46.HHr at phibred.com>, bipin dalmia <dalmiabk at phibred.com> writes:
>[...]
>centrifugation at 20,000 g for 20 minutes and NO PELLET!!! can someone
>explain what happened and how i can recover my protein?
>bipin

I think keeping your mixture on ice for at least an hour will improve things.
Maybe you should also increase your G-force!

Flip



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