Enzymes in KGB?
salger at wap18.zi.biologie.uni-muenchen.de
Fri Oct 21 18:52:06 EST 1994
McClelland et al. described a K-glutamate buffer (KGB) in which
a lot of different restriction enzymes, Klenow, T4 DNA polymerase,
and T4 DNA Ligase are active. But there are some more enzymes of
interest, phosphatases (e.g. SAP, CIP), nucleases (e.g. mung bean, S1,
ExoIII), other polymerases (e.g. Taq, T7 DNA polymerase), in general
all enzymes which are used to modify DNAs and perhaps other enzymes, too.
I would appreciate if people who tried KGB with enzymes other than the
published ones would post their experiences.
P.S. the reference is "McClelland et al. (1988) NAR Vol 16 No 1, p.?"
Klaus Salger phone : ++49 (0)89 5902 -502
Zoologisches Institut FAX : -450
AG MacWilliams e-mail: salger at zi.biologie.uni-muenchen.de
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