Inverse PCR

TIM ELLS ells at NSRSKE.AGR.CA
Sat Oct 22 09:16:27 EST 1994


Hi everyone,
		I've been following the recent thread on inverse 
PCR and have one question. If you want to ligate (circularize) a
fragment with 'sticky ends', what is the size limit of the DNA
fragment? I want to circularize a 15 kb fragment. Is this too 
large? 

Tim Ells




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