direct sequencing of PCR products

Steve Marsh marshs at cco.caltech.edu
Sat Oct 22 17:58:56 EST 1994


II have sequenced from PCR products using the ABI Dye Primer and Dye
terminator cycle sequncing kits. The samples were gel purified and about
150 nmoles were recovered. The cycle conditions were straight out of the
protocol and we got 350-500bp reads on the 373 stretch depending on the
size of the PCR product.
Steve Marsh
DNA Sequencing Core Facility at Cal Tech.



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