Help-plasmid recovery from dead plate!

Tara McCready Tara_McCready at
Sun Oct 23 16:48:31 EST 1994

Help - I need to try to recover my plasmid from a LBAmp plate (one month
old) covered with very dead E coli.  I know that this has been posted
before, but I didn't save the articles and now I'm stuck without even a
microlitre of my purified plasmid.  Right now I'm trying to grow some of
the cells from the plate in LB at 37C before plating them out on LBAmp, but
I'm not optimistic!   Thanks in advance for your help - maybe this should
be in FAQ!

Tara McCready
Dept of Biochemistry
University of Alberta

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