Sal I cloning problems

Lisa Taylor ltaylor at
Mon Oct 24 18:26:57 EST 1994

                       Subject:                               Time:3:13 PM
  OFFICE MEMO          Sal I cloning problems                 Date:10/24/94
I have had a great deal of trouble cloning anything which involves a Sal I site.
 I would like to know if anyone else has had trouble with this endonuclease and
more importantly, if anyone has found a way to digest with Sal I and then get
successful ligations.  I have been using Sal I from NEB.

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