Ghosts and membrane fluidity

Jan Palaty jpalaty at
Mon Oct 24 15:23:13 EST 1994

Does anyone have any experience with measuring membrane fluidity in 
erythrocyte ghosts using fluorescence polarization?  Specifically, are 
there any special tricks to preparing ghosts for this purpose?  I've been 
trying to reproduce earlier literature results (as well as some of my 
own) on the membrane-disordering effects of some simple acids without any 
luck.  A wash with PBS/lyse with hypotonic buffer/wash with PBS approach 
is being used.

Any help would be greatly appreciated!  Thanks.

Jan Palaty
Faculty of Pharmacy

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