Nonrad. ATP synthase assays?

Ferenc Marincs agpfxm at pnv.palm.cri.nz
Tue Oct 25 15:03:57 EST 1994


In article <cc37-141094172635 at 132.236.171.168>, cc37 at cornell.edu (CConley)
wrote:

> Hello --
>       I'm trying to characterize the activity of ATP synthase from isolated
> mitochondria. I'd like to use a non-radioactive assay -- I've heard of a
> luciferase assay, where one adds luciferase, substrates, and ADP to the
> mitochondria and then measures the fluorescence.  Is this a good assay,
> what might be problems with it, and are there other assays which I should
> consider (commercial products)?
>       Thanks,
>                                                -- CConley

aa

-- 
Ferenc Marincs
AgResearch, Private Bag 11008, Palmerston North, New Zealand
Phone: +64-6-356 8019, Fax: +64-6-351 8032
E-mail: agpfxm at pnv.palm.cri.nz



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