Nonrad. ATP synthase assays?
agpfxm at pnv.palm.cri.nz
Tue Oct 25 15:03:57 EST 1994
In article <cc37-141094172635 at 18.104.22.168>, cc37 at cornell.edu (CConley)
> Hello --
> I'm trying to characterize the activity of ATP synthase from isolated
> mitochondria. I'd like to use a non-radioactive assay -- I've heard of a
> luciferase assay, where one adds luciferase, substrates, and ADP to the
> mitochondria and then measures the fluorescence. Is this a good assay,
> what might be problems with it, and are there other assays which I should
> consider (commercial products)?
> -- CConley
AgResearch, Private Bag 11008, Palmerston North, New Zealand
Phone: +64-6-356 8019, Fax: +64-6-351 8032
E-mail: agpfxm at pnv.palm.cri.nz
More information about the Methods