DNA/phenol problem

MAD, MAD, MAD SCIENTIST jwg at gnv.ifas.ufl.edu
Tue Oct 25 13:33:37 EST 1994


In article <38ftvv$c2 at er1.rutgers.edu>, jwelsh at er1.rutgers.edu (Jennifer_ Welsh) writes:
> Dear Netters,
> 
> I've been doing a standard (mod. Birnboim and Doly) alkaline lysis DNA
> prep on 40ml E. coli cultures.  All of a sudden I was having trouble
> getting a decent yield.  I did the protocol in parallel using my
> solutions and another person's.  An agarose gel shows the yield to be
> the same before phenol extraction but not after (the same phenol was
> used for both preps).  Does anyone have any ideas about what is going
> wrong?  I checked the pH of the NaOAc.  It's correct.  All advice
> gratefully accepted.
> 
> Thanks in advance,
> 
> Jennifer Welsh
> 
> -- 
> If you want me, look at welsh at biovax.rutgers.edu

Is your Phenol Tris equilibrated?  If not this may be the root of
the problem

Jay





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