incorporating biotin-dNTPs

schoenfeld at MSSCC.MED.UTAH.EDU schoenfeld at MSSCC.MED.UTAH.EDU
Tue Oct 25 17:26:40 EST 1994


Hi -
Has anyone tried to incorporated biotinylated dNTPs w/T4 polymerase or
Klenow?  I'm trying to chew off 10 bases and create a specific overhang
before adding the biotinylated dNTP.  Any protocols would be greatly
appreciated.

Thanks
Robert Schoenfeld
Univ. Utah
schoenfeld at msscc.med.utah.edu 




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