Detection of "Invisible" Proteins??? - Reply

Varyc varyc.mmcri at OFFICE.MMC.ORG
Tue Oct 25 05:42:01 EST 1994

re aromaticless polypeptide:
	Does anyone know of a protein concentration method that doesn't 
require aromatic AA's?  We can't really run this on SDS-PAGE and it seems
that the Bradford assay also needs aromatics.  

Peptide bonds have decent extinctions in the 220-230nm range. Scan there to
see if your machine can handle these energies, use very clean (filtered,
centrifuged) solutions/solvents to avoid end absorbtion.

Cal Vary

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