Detection of "Invisible" Proteins??? - Reply
varyc.mmcri at OFFICE.MMC.ORG
Tue Oct 25 05:42:01 EST 1994
re aromaticless polypeptide:
Does anyone know of a protein concentration method that doesn't
require aromatic AA's? We can't really run this on SDS-PAGE and it seems
that the Bradford assay also needs aromatics.
Peptide bonds have decent extinctions in the 220-230nm range. Scan there to
see if your machine can handle these energies, use very clean (filtered,
centrifuged) solutions/solvents to avoid end absorbtion.
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