RT storage?

croyleml at ucbeh.san.uc.edu croyleml at ucbeh.san.uc.edu
Wed Oct 26 14:33:14 EST 1994


Does anyone have any experiance with the best way to store
Reverse Transcriptase (RT) reaction product (cDNA)?  We
are doing large scale RT reactions to have enough material
to do many PCR reactions over a extended period of time.
It is very important for our work that we have as little 
degradation of the cDNA as possible.  What are the most 
important factors: Temp (-20 vs. -70); Frezze thaw cycles;
Will RNAseH degrade cDNA over time;.....

Thanks




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