need protocol for pcr of phage from plates.

Mandalaparthy padmmv00 at
Wed Oct 26 22:42:47 EST 1994

Dear netters,

It would be a great help if any one has a simple protocol to pcr an insert
out of a lambda gt10 vector directly from the plaques on a plate. If you
give a elaborate protocol with temps etc it would be really helpful. I am
a grad student working in a smallish dept so outside help is extremely
valuable. Please e-mail or post. My e-mail address is at

Padmaja Mandalaparthy
grad student
usf,  CA.

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