PCR crash

[Kevin Morano]

PANIAGUA SOLIS JORGE FERNANDO (jpaniagf at CONDOR.DGSCA.UNAM.MX) wrote:
: Hello, colleages.
: I am stuck with an amplification:

20 mM MgCl2 is a LOT of Mg for a normal reaction. Have you tried 
titrating it down from there to, say, 1-4 mM??

Good luck!
Kevin

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Kevin A. Morano
Internet:kamorano at ucdavis.edu    
Bitnet:kamorano at ucdavis   
Section of Microbiology, University of California, Davis