DNA/phenol problem

Andre Hamel hamel at cc.umanitoba.ca
Wed Oct 26 18:01:39 EST 1994


Regarding NaOH-SDS plasmid preps (Birnboim & Doly) ... is the NaOH stock
stored in plastic? ... better NOT be stored in glass, elsewise conc of
hydroxyl increases along with Na-Si ppt ... buggers up more than just alk
lysis preps.

Regards phenol ... include 0.1% hydroxyquinoline (yellow powder) ...
yields yellow phenol that will oxidize less rapidly ... also, phenol best
stored frozen (in convenient sized aliquots ... several 1, 5, 10 & 50 mL
aliquots, depending on volumes used at a time).

cheers,


                            ********************
Andre Hamel                                    email: hamel at cc.umanitoba.ca
Manitoba Government				tel: 204/945-7630
Department of Agriculture  			FAX: 204/945-8062
Veterinary Services Branch          
Winnipeg, Manitoba, CANADA 
			  ********************




More information about the Methods mailing list