urea/SDS, guanidine/SDS PAGE

gflentke.internet{gflentke at facstaff.wisc.edu} gflentke.internet{gflentke at facstaff.wisc.edu}
Thu Oct 27 15:37:37 EST 1994


  To: methods-and-reagents at net.bio.net
  From: gflentke at facstaff.wisc.edu
  Reply-To: gflentke at facstaff.wisc.edu

In <38n5b8$mef at newsbf01.news.aol.com>, hschreiner at aol.com (HSchreiner) writes:
>Is there a standard protocol to follow when running protein samples that
>have been solubilized in 6M guanidine hydrochloride (Gdn-HCL) on
>SDS-polyacryamide gels?  Are urea/SDS polyacryamide gels ever used?  What
>about Gdn-HCL/SDS-polyacrylamide gels?  Also, how does one go about
>treating the protein sample in Gdn-HCL prior to loading on to the ge?  Any
>suggestions would be appreciated.   Paul  (GonchaPa at umdnj.edu)

Urea/SDS Gels are used in the Schagger-Von Jagow procedure to improve resolution
of smaller proteins.  Gdn-HCl is a charged compound and large quantities in the 
gel are not recommended.  Even the amounts in the loading sample can cause major
distortions.  It can be done.  You will have to titrate out your system to find 
out how much your system can handle.

GRF






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