What's the best condition for transfection of most commonly used cell lines?

Aurel d'etre hongyang at husc7.harvard.edu
Wed Oct 26 22:12:38 EST 1994

NIH3T3, CV1-P, SAOS-2, and U2OS cell lines, what's the optimal pH of HBS 
buffer, and what's the best time to harvest cells for transient assay?
(Calcium Phosphate preciptation method)
Anyone has successful experience with her/his particular cell lines may
share the parameters so that I can compile a set of conditions for each
cells and I will post the collection to this group.


email: hyang at mbcrr.harvard.edu or hongyang at fas.harvard.edu
617-739-3276(H) 632-4378(Lab)

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