HA epitope tag problems

PJOB obrien at pharm.med.upenn.edu
Thu Oct 27 11:00:44 EST 1994


We've been trying to tag a g-protein coupled receptor with the HA epitope
recognized by the 12CA antibody, but have been unable to immunoprecipitate
or Western blot the bugger.  The DNA sequence looks o.k., but I've heard
rumors about others having difficulty with this tag.  Does anyone have any
insights?



Peter O'Brien
Dept. of Pharmacology
U. of Penn.



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