Protocal for DNA extension

Alex Chang lpss at
Fri Oct 28 22:17:37 EST 1994

Dear netters:

I am looking for a good protocal for doing DNA extension. 

I have two pieced of synthetic oligos, one 34 bases long, the other 110 
bases, and they are have 18 bases complementary each other. They are 
something like this:

5'__________________ 3'

I'd like to extend them to make a dsDNA and wondering what is the best 
DNA polymerase I should use.

Alex Chang
University of British Columbia
achang at

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