Protocal for DNA extension

Alex Chang lpss at unixg.ubc.ca
Fri Oct 28 22:17:37 EST 1994


Dear netters:

I am looking for a good protocal for doing DNA extension. 

I have two pieced of synthetic oligos, one 34 bases long, the other 110 
bases, and they are have 18 bases complementary each other. They are 
something like this:

5'__________________ 3'
             3'-----------------------------------------5'

I'd like to extend them to make a dsDNA and wondering what is the best 
DNA polymerase I should use.

Regards
--
Alex Chang
Pathology
University of British Columbia
achang at hivnet.ubc.ca



More information about the Methods mailing list