Amplification of pACYC184 plasmid

jalexand at jalexand at
Fri Oct 28 10:38:31 EST 1994

I cannot obtain any amount of plasmid DNA when i try to amplify in DH alpha 
mcr E. coli strain pACYC184 plasmid using or not spectinomycin.
Is there any special E. coli strain to use for the amplification?
have you any idea about this bad result?
Thank you for the answer.

More information about the Methods mailing list