Quantitative protein determinations in plant extracts

bipin dalmia dalmiabk at phibred.com
Fri Oct 28 14:19:46 EST 1994


what is an absolute method of protein determination in plant extracts
besides gravimetric analysis? in particular, does anyone have data on how
nitrogen combustion, Kjeldahl or any other method compare when
determining total protein content in any kind of crude preparations? we
are getting pretty large differences between lowry (folin), BCA and
bradford assays. these were done with BSA as a standard, and all buffer
inteferences were eliminated.

bip



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