Detection of "Invisible" Proteins??? - Reply
dmcbtch at ucl.ac.uk
Fri Oct 28 12:41:25 EST 1994
In article <BDA7AC2E01B938D9 at office.mmc.org>
varyc.mmcri at OFFICE.MMC.ORG (Varyc) writes:
> re aromaticless polypeptide:
> Does anyone know of a protein concentration method that doesn't
> require aromatic AA's? We can't really run this on SDS-PAGE and it seems
> that the Bradford assay also needs aromatics.
> Peptide bonds have decent extinctions in the 220-230nm range. Scan there to
> see if your machine can handle these energies, use very clean (filtered,
> centrifuged) solutions/solvents to avoid end absorbtion.
> Cal Vary
Oh, it hurts so much.
Try a Lowry assay (or Piece's BCA which is also a Cu(2+) ->Cu(1+) based
MRC Lab for Mol. Cell Biology
University College London
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