Detection of "Invisible" Proteins??? - Reply

[Tom Chappell]

In article <BDA7AC2E01B938D9 at office.mmc.org>
varyc.mmcri at OFFICE.MMC.ORG (Varyc) writes:

> re aromaticless polypeptide:
>         Does anyone know of a protein concentration method that doesn't 
> require aromatic AA's?  We can't really run this on SDS-PAGE and it seems
> that the Bradford assay also needs aromatics.  
> 
> 
> Peptide bonds have decent extinctions in the 220-230nm range. Scan there to
> see if your machine can handle these energies, use very clean (filtered,
> centrifuged) solutions/solvents to avoid end absorbtion.
> 
> Cal Vary
> MMCRI

Oh, it hurts so much.

Try a Lowry assay (or Piece's BCA which is also a Cu(2+) ->Cu(1+) based
assay).


Tom Chappell
MRC Lab for Mol. Cell Biology
University College London