Detection of "Invisible" Proteins??? - Reply

Tom Chappell dmcbtch at
Fri Oct 28 12:41:25 EST 1994

In article <BDA7AC2E01B938D9 at>
varyc.mmcri at OFFICE.MMC.ORG (Varyc) writes:

> re aromaticless polypeptide:
>         Does anyone know of a protein concentration method that doesn't 
> require aromatic AA's?  We can't really run this on SDS-PAGE and it seems
> that the Bradford assay also needs aromatics.  
> Peptide bonds have decent extinctions in the 220-230nm range. Scan there to
> see if your machine can handle these energies, use very clean (filtered,
> centrifuged) solutions/solvents to avoid end absorbtion.
> Cal Vary

Oh, it hurts so much.

Try a Lowry assay (or Piece's BCA which is also a Cu(2+) ->Cu(1+) based

Tom Chappell
MRC Lab for Mol. Cell Biology
University College London

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