Homemade GeneClean kits ???
ez001427 at dale.ucdavis.edu
Sun Oct 30 18:21:27 EST 1994
fnord (jpa6r at faraday.clas.Virginia.EDU) wrote:
: I was told that some labs make their own reagents for
: silicon based DNA purification (i.e., GeneClean kits) including the
: glass beads. Does anyone know of any protocols? I would appreciate
: any pointers to the original literature describing the method.
: jpa6r at virginia.edu
I've never made the glass beads before, but I have had opportunity to
make up a batch of the sodium iodide. First time I tried it, my yield
was terrible. I was then recommended to filter it through a very thick
layer of glass fiber filters. After doing this, the solution worked just
fine. I presume there is a contaminant in commercially available sodium
iodide that interferes with the DNA-glass binding. Filtering through
GFFs titrates out this contaminant.
Section of plant Biology
University of California Davis
magoldstein at ucdavis.edu
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