Methods October 1994 Archives by author

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Starting: Sat Oct 1 07:08:14 EST 1994
Ending: Mon Oct 31 23:05:18 EST 1994
Messages: 1221

test Cheng Luo #3295
luminous pen instead of radioactivity Stefan Gruenert (WCI)
lexA-antibody
Wanted a mentor from industry Labuser 1
Why MOPS for RNA gels? D.Greeve <DAEMON>
non title 額賀路嘉
(none) Anders Bäckström
(none) Anders Bäckström
DNA-RNA hybrid cross-linking León Fernando García-Martínez
ab:6xHisTag AKravetz
minipreps for sequencing AKravetz
get rid of developer stains from clothing AMBION at DELPHI.COM
Stool RT-PCR Dale Yuzuki's Work Account
Long range Genomic PCR Dave Adelson
35S-Met lifetime? Dave Adelson
Assymetric PCR references Ashok Aiyar
Acrylamide electrophoresis of DNA problem Albert
chemistry of Qiaex beads & DNA interaction?? Shane Albright
protein precipitates:HELP ! Shane Albright
Need address for Molecular Probes Inc. Stephen B. Aley
DNA sequencer Eric C. Anderson
promega wizard kits and the 373 Eric C. Anderson
test, sorry to bother Eric C. Anderson
Which PCR machine? Eric C. Anderson
Best strategy from peptide sequences to cDNA clone? Eric C. Anderson
luminous pen instead of radioactivity Eric C. Anderson
Codon preferences of differrent organisms Eric C. Anderson
methylmercuric hydroxide Azlinda Bte Anwar
Phosphoimagers vs Instantimager Tracy Aquilla
RT-PCR question Tracy Aquilla
Why MOPS for RNA gels? Tracy Aquilla
Phosphoimagers vs Instantimager Tracy Aquilla
GeneClean versus Quiagen Tracy Aquilla
EXONUCLEASE III Tracy Aquilla
EXONUCLEASE III Tracy Aquilla
ANCIENT DNA Tracy Aquilla
ANCIENT DNA Tracy Aquilla
Where to buy genomic libraries? Tracy Aquilla
Protein Purification - Woe is me!!! Curt Ashendel
urea/SDS, guanidine/SDS PAGE Curt Ashendel
Why MOPS for RNA gels? Jim Astwood
Phosphoimagers vs Instantimager Graham Atherton
Luciferase as internal control Graham Atherton
Disappearing retard band Graham Atherton
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" BAI_JN at JHUVMS.HCF.JHU.EDU
help! purifying polyclonals BARKER, SHARON L.
staining for beta-galactosidase BMA14432 at vax1.utulsa.edu
Homebrew saline for Contact Lenses Shawn C. Baker
Protein FPLC (HPLC) Systems Clifford Beall
isotec Clifford Beall
PCR of GC-rich genes Clifford Beall
35S labeling E coli Michael Benedik
PCgene program Benoit_Hebert at IAF.UQUEBEC.CA
WANTED: Old liquid scintillation counters Ron Berry
Cosmid sequencing with the 373 Lisa Bibbs
IgM Shaun D. Black
ATCC site Shaun D. Black
Free sulfhydryl determination Shaun D. Black
Ellman's Reagent/DTNB (PROTOCOL) Shaun D. Black
Adding membrane signal to cytoplasmic protein Shaun D. Black
48-well plate tools? John E. Blume
Geiger Counter Peter T. Boag
txn factor consensus seq. database?? Jeffrey H. Boatright
transcription+translation Peta C. Bonham-Smith
c-myc expression system Peta C. Bonham-Smith
ABI sequencing and Stratagene Taq Extender Dirk Bornemeier
Do you have ABI Sequenase Kit problems??? Dirk Bornemeier
E. coli lacZ complementation Chris Boyd
Coupling IgG via sugar residues Tomas Bratt
How to Purify Glyc. Dehyd? Dave Bremer
ANNOUNCE: USED ANALYTICAL LAB EQUIPMENT FOR SALE John Brookes
Buffer for plaque phage lysis --> PCR ? Stuart Brown
What is the perfect PCR? Stuart Brown
Hair Follicle DNA Fingerprinting Karen Browning
Info. on refrigerated microfuge John Brunstein
Problems w/ native PAGE Paul A Bucciaglia
Problems w/ native PAGE Paul A Bucciaglia
Aggregation of membrane proteins Andreas Buhr
Need address for Molecular Probes Inc. Sebastian W. Bunka
Hybaid products Sebastian W. Bunka
simple protocol for colony PCR Sebastian W. Bunka
ELISA plates and protein adsorbtion Emanuele Buratti
Optimal Conditions for Boehringer PWO enzyme in PCR Jonathan Patrick Burke
screening plasmid libraries Jon Burrows
In search of the "Holy manual" Timothy Bushnell
help for oligo hybridization Timothy Bushnell
What's the best mini-prep? C.J. Hussussian, MD
Nonrad. ATP synthase assays? CConley
0.02 filters CURTIS at UTMSI.ZO.UTEXAS.EDU
35S labeling E coli Paul Campbell
wanted: 'Simple Fool's guide to PCR' Dave Carmean
Codon Usage and Expression in E. coli Eric Carstens
DNA/phenol problem Chuck Cerveny
Cheaper electroporation cuvettes? George W Chacko
Adding membrane signal to cytoplasmic protein George W Chacko
Protocal for DNA extension Alex Chang
Detection of "Invisible" Proteins??? Jon Chappel
Best method for blunting ds DNA? Tom Chappell
Detection of "Invisible" Proteins??? - Reply Tom Chappell
Hot phenol prep, genomic DNA David Char
long ranger gels Mic Chaudoir
ANCIENT DNA Mic Chaudoir
Cheaper electroporation cuvettes? Mic Chaudoir
Homebrew saline for Contact Lenses Mic Chaudoir
How good is the Eagle Eye II ? Alternatives ? Mic Chaudoir
help for oligo hybridization Mic Chaudoir
Western Using Monoclonals Mic Chaudoir
neosystem Michelle Chen
pGRE5.1, the inducing condtion Shu-Chih Chen
RDA Frank Cheng
DNA purification Chutney
ABI sequencing and Stratagene Taq Extender Duncan Clark
Thermus filiformis DNA polymerase Duncan Clark
PacI weirdness Duncan Clark
PacI weirdness Duncan Clark
Expression problems Duncan Clark
Codon Usage and Expression in E.coli. Duncan Clark
E. coli lacZ complementation Duncan Clark
Codon Usage and Expression in E.coli. Duncan Clark
Expression problems Duncan Clark
Bulk prep problems Duncan Clark
DISCUSSION - plasmid piracy = software piracy ? Duncan Clark
Transfection of oesophageal (squamous cell)carcinoma cell line Volkert Clausen
Polysomes preparation Michael Coady
What's in Megascript buffer ? Michael Coady
Adding membrane signal to cytoplasmic protein Michael Coady
35 mm slides of gels - help Andrew Cockburn
ANTIBIOTIC STORAGE Andrew Cockburn
Phosphoimagers vs Instantimager Randall J. Cohrs
Companies on the Net Randall J. Cohrs
PHAGE DNA KITS? Randall J. Cohrs
>>>Help about formal/agarose gel <<< Randall J. Cohrs
Info. on refrigerated microfuge Randall J. Cohrs
teflon tape Randall J. Cohrs
Help need for Single-step RNA Ioslation by Acid Guanidinium Randall J. Cohrs
DISCUSSION - plasmid piracy = software piracy ? Randall J. Cohrs
Taq left at RT - gulp! - Susan Cole
WANTED: Old liquid scintillation counters Matthew J. Combs
Help: Program to find quasi-restriction sites Juan Antonio Contreras
NaAc precipitation Michael Cooley
problem with pUC18..help me !! Michael Cooley
Buffering sat'd NaI sol'ns Michael Cooley
DNA/phenol problem Michael Cooley
Fluorometer Michael Cooley
dead cells Seth Corey
DNA extraction: Lactob. Heitor Luiz da C. Coutinho
protein/nucleic acid motifs Dr. P.A. Crossey
B.M.s 5 minute ligations? DAB
>>> Help about formal/agarose ges <<< PHIL DANIEL
Chemical cleavage of mismatches (CCM) Bill Oetting DGM-IHG
non-radioactive DNA sequencing MARIA ELENA GARCIA DIAZ
Subscription Slawek Dabrowski
RNA from blood Richard Dana
Need help With Blood RNA prep Richard Dana
Recent DNA and RNA isolation methods wanted Richard Dana
cDNA library from wheat and soybean Richard Dana
Some Receptor Theory Questions...Constitutive or not? Dave
Type 52 Polaroid films? Who?****** David L. Haviland, Ph.D.
promega wizard kits and the 373 David L. Haviland, Ph.D.
ANTIBIOTIC STORAGE David L. Haviland, Ph.D.
HELP!!! Electroporation and PEG David L. Haviland, Ph.D.
Prokaryotic fusion protein systems Simon Dawson
RT-PCR question Simon Dawson
SDS Problem Simon Dawson
Software to call heterozygosity from ABI output Ron Debry
Pharmacia GeneQuant DNA calculator opinions wanted Greg Denomme
cDNA ()TPR1) wanted Thomas Deufel
RT-PCR in NuSieve ??? Michael Deyholos
POTATO SOMATIC MUTATIONS Dickinson, Harry
Non-denaturing Southern Blotting Scott J. Diede
spectrophotomer Therese Ann Dietlin
What is the perfect PCR? John Dixon
Enough Re: Promega on the Internet Reinhard Doelz
Homebrew saline for Contact Lenses Donald A. Lehn, Ph.D.
Arabidopsis cDNA Iain Donaldson
Genomic DNA from E.coli without phenol or chloroform?? Gabriel Dorado
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? Gabriel Dorado
Which PCR machine? Peter Doris
Dictionary of Cell Biology on WWW ! Julian Dow
Buffering sat'd NaI sol'ns Virginia Dress
Detection of "Invisible" Proteins??? Virginia Dress
Reverse northerns,cDNA not hot enough. Dr. YL Du
DNA from formalin preserved tissue Thomas F Duda
Transfection of neuronal cell lines??? CARPENTER E.
Green Fluorescent Protein CARPENTER E.
pRc/CMV containing lacZ CARPENTER E.
Transfection of neuronal cell lines??? CARPENTER E.
PCR produced probes TIM ELLS
Inverse PCR TIM ELLS
Phosphoimagers vs Instantimager Nigel Eastmond
Strength of 4ml Spec. Cuvettes Nigel Eastmond
Test George F Edick
Growing E. coli George F Edick
Keystone Oncogenes mtg: Want Roommate Steve Eklund
VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY Bruce James Ella
Artifacts in silver staining Bruce James Ella
VOLATILE BUFFERS Bruce James Ella
DIG system for Northern blotting Bruce James Ella
DIG system for Northern blotting Elizabeth Estabrook
PCR crash PANIAGUA SOLIS JORGE FERNANDO
protein aggregation FORSDYKE at QUCDN.QueensU.CA
? Supplier of mRNA Diff. display kit Patrice Ferriola
William D. Huse / Ixsys, Inc -- San Diego, CA H. David Fischer
PFGE and YACs Karl Fischer
Detection of "Invisible" Proteins??? Karl Fischer
RTPCR/degenerate primers with inosine? Cynthia Fisher
exonuclease I Parke K. Flick
Phosphoroimagers vs Instantimager Experiences Saught Brian Foley
PCR fidelity: Calculations? Brian Foley
txn factor consensus seq. database?? Brian Foley
Inosine-containing oligos John E. Fox
oligonucleotide synthesizers John E. Fox
Were we can buy cheapest DNA primers? John E. Fox
Xenopus downtime Sherri Fraser
NaAc precipitation Sherri Fraser
Luminous Pens Sherri Fraser
Luciferase Problems? Sherri Fraser
6X myc cassette request Lorenzo Frigerio
Luminescent vs fluorescent ELISA James L Funderburgh
Are Ab's phosphorylated? Michele Fuortes
Non-radioactive run on Michele Fuortes
Idaho Technology RAPD analysis Anthony V. Furano
antibody to luciferase commercially available? G.E.Fazzi
Artifacts in silver staining G.J.W.Euverink at biol.rug.nl
internet for the mol. biologist GRIFFINHG
hybridization bags Prasit Palitpolkanpim - GRSO
Which PCR machine? G Gallagher
long film & cassettes for sequencing - where? Frederick Garbrecht
PCR journal Frederick Garbrecht
Artifacts in silver staining Frederick Garbrecht
Long gel dryers Frederick Garbrecht
Western Using Monoclonals Frederick Garbrecht
QUES: Galvanic Skin Response Todd G Gardner
Pichia single-copy gene Nicholas Gascoigne
Transfection of CaCo-2 cells Ane Gedde-Dahl
PC-based plasmid map programs Peter Gegenheimer
Storage of RNA Peter Gegenheimer
Glycogen James Gibbs
NuSieve Agarose Michael Gieseg
Bulk prep problems Michael Gieseg
S1 nuclease/ exo III Fabrice Glibert
dye in PCR Fabrice Glibert
Maybe a stupid PCR ques Fabrice Glibert
Which PCR machine? Dr. Greg Gloor
PCR gene splicing: help! Greg Gloor
cDNA kits? Greg Gloor
? Supplier of mRNA Diff. display kit David Glover
Homemade GeneClean kits ??? Marc Goldstein
Mouse intestine cDNA library Richard Gomer
betagalactosidase antibodies David Gonda
PCR PRIMERS Efram Gonzalez
is there an index, restr. enz. sources??? Anita Gould
pCMV5 sequence ? Roger Graham
RNA structure graphics software Jerry Grandoni
Nonrad. ATP synthase assays? Brian T. Greuel
ATCC site Neil Griffin
Enzymes in KGB? Duke Groebe
QUICKHYBE from Stratagene - opinions? Duke Groebe
NIH-3T3 drug test Jody Groenendyk
SUBSCIBTION H1411Mus at HUELLA.BITNET
Ext. of DNA from agarose (which agarose) HARDIES at THORIN.UTHSCSA.EDU
ss. DNA restriction (yes, they cut) HARDIES at THORIN.UTHSCSA.EDU
SDS problem (for bkg, not stringency) HARDIES at THORIN.UTHSCSA.EDU
Best strategy pep. seq. -> cDNA HARDIES at THORIN.UTHSCSA.EDU
NaAc pptn. HARDIES at THORIN.UTHSCSA.EDU
DNA insoluble in water HARDIES at THORIN.UTHSCSA.EDU
Bulk prep problems (Yes, plasmids can integrate) HARDIES at THORIN.UTHSCSA.EDU
oligos on membranes HOWARD at MGRP.BGSM.WFU.EDU
oligo-binding membranes HOWARD at MGRP.BGSM.WFU.EDU
sections of hard tissues falling off HRMBDKC at HKUMD1.HKU.HK
Hybaid products HRMBDKC at HKUMD1.HKU.HK
urea/SDS, guanidine/SDS PAGE HSchreiner
DNA-Extr. of a Histological Cut H Rochholz/ H Haas
teflon tape H. Rochholz/H. Haas
PFGE and YACs Antje Haase
(none) S.Mitchell Halloran
urinary iodine determn; water qual. anal. with cap. elect. S.Mitchell Halloran
Topology of a multisubunit membrane protein Tuomas Haltia
Extraction of DNA from agarose gels Andre Hamel
5'->3' InhibitACE Andre Hamel
Storage of RNA Andre Hamel
Riboprobe background hybridisation Andre Hamel
Maybe a stupid PCR question & a ETBr question Andre Hamel
DNA/phenol problem Andre Hamel
Primers NOT needed for RT rx'n?!?! Andre Hamel
YY1 Tan Shyh Han
NruI Johannson Hans
densitometry software Terry Hanzlik
RT-PCR question Richard R. Hardy
Ambion RPA II Kit?? Jane Harrison
Extraction of DNA from agarose gels Yasha Hartberg
pcDNA3 with Wizard Yasha Hartberg
Kunkel mutagenesis- Help needed Yasha Hartberg
Kunkel mutagenesis- Help needed Yasha Hartberg
temperature-dependence of DNA-gyrase Yasha Hartberg
EtBr-again Yasha Hartberg
Problems w/ native PAGE Yasha Hartberg
Spheroplast Philip Heacock
Yeast gene expression in mammalian cells Philip Heacock
Inverse PCR Dr. C.P. Healy
Determination of genome size (Cot curves) Dr. C.P. Healy
Inverse PCR Dr. C.P. Healy
Chicken typeII collagen probe Dr. C.P. Healy
Radiographic Duplicating Unit Steven Hecht
Phosphoimagers vs Instantimager Peter Heifetz
SDS Problem Peter Heifetz
Phosphoimagers vs Instantimager Peter Heifetz
Phosphoroimagers vs Instantimager Experiences Saught Peter Heifetz
Phosphoimagers vs Instantimager Peter Heifetz
cDNA library screens via PCR Lynn M Heltemes
Capsaicin Scott Selby Med Heme
EGF repeats in E.coli? Sam Henderson
Ab for 6XHis tag?? Sam Henderson
RT-PCR question Too Heng-Phon
Glowing BLUE DNA Gels Paul N Hengen
Best Way Clone PCR Products Paul N Hengen
What's the best mini-prep? Paul N Hengen
Taq left at RT - gulp! - Paul N Hengen
Promega on the Internet Paul N Hengen
pcDNA3 with Wizard Paul N Hengen
Glowing BLUE DNA Gels Paul N Hengen
Dye to colour DNA samples for PCR Paul N Hengen
Extraction of DNA from agarose gels Paul N Hengen
Frequently Asked Question (FAQ) list Paul N Hengen
freeze-sqeeze Paul N Hengen
Green Fluorescent Protein Paul N Hengen
Storing PK\LDH without any sulfate ? Jean-Manuel Henry
Antibodies to KDEL or kXXkx peptides Your Name Here
request advice on RNA storage Robert Hewitt
request advice on mRNA preparation Robert Hewitt
GST Bernard Heymann
Protein Purification - Woe is me!!! Andy Hill
address of So. Cal forensic lab Walter Hill
CAPILLARY ELECTROPHORESIS equipment ? Timo Hiltunen
Minipreps for ABI sequencer Robert Hindges
Amino Acid Analysis software on www Uwe Hobohm
luminous pen - use GLOGOS Tony Hodge
Need reliable method for RNA extraction from lymphocytes Chris Hodgson
Topology of a multisubunit membrane protein Flip Hoedemaeker
ammonium sulfate precipitation of proteins Flip Hoedemaeker
ANCIENT DNA Guy Hoelzer
DNA Ladders for Short Fragments Guy Hoelzer
Denatured DNA and fixation Guy Hoelzer
cDNA Challenge************* Annette C. Hollmann
RT-PCR question Annette C. Hollmann
Thermocyler runs Annette C. Hollmann
Western Using Monoclonals Annette C. Hollmann
protease info / database Gene Holowachuk
32P labeling tumors in vivo Gene Holowachuk
rBGH organism Louis Hom
Detergents and Disruption Louis Hom
nuclear melanin Louis Hom
Invitrogen FTP/Gopher Site Christopher Hoover
Promega on the Internet Christopher Hoover
Methylmercuric Hydroxide Christopher Hoover
Dye to colour DNA samples for PCR Robert Horton
test David Hosfield
LacSwitch sequence Eivind Hovig
Advse needed on DNA strand-separation Baohua Huang
Antibody against neomycin phosphotransferase David S. Huen
In search of the "Holy manual" David S. Huen
Why MOPS for RNA gels? David S. Huen
LacZ in eukaryot. promoter studies David S. Huen
>>> Help about formal/agarose ges <<< David S. Huen
COT 1 preparation! HELP!!! David S. Huen
protein aggregation Timothy R. Hughes
Homebrew saline for Contact Lenses Eric R. Hugo
retroviral DNA preps Olav Hungnes
35S labeling E coli Arthur Hunt
Why MOPS for RNA gels? Keith Hutchison
genomic dna prep Darin Obrien IHG
Urbino Meeting...info needed The doctor is IN.
>>>Help about formal/agarose gel <<< CJF 90/10 INSERM
RNAse free water? CJF 90/10 INSERM
Polysomes preparation CJF 90/10 INSERM
GeneClean versus Quiagen CJF 90/10 INSERM
----URGENT : RNAase Free Water ?---- CJF 90/10 INSERM
---- Polysomes preparation ---- CJF 90/10 INSERM
Green Fluorescent Protein J.Carnwath
Free BioTechniques Past Paper Database J.Carnwath
anti-Taq polymerase antibodies for hotstart J.Carnwath
address for Dynatech? J.Carnwath
Green Fluorescent Protein J.Carnwath
HOMOLOGOUS TRANSFECTION SYSTEM MUCHA JAN
Companies on the Net JEMERTON at BIOTECHNET.COM
Free BioTechniques Past Paper Database JEMERTON at BIOTECHNET.COM
Free BioTechniques Past Paper Database JEMERTON at BIOTECHNET.COM
inducible drug resistance JKHLab
inducible drug resistance JKHLab
Biotrace membrane - Gelman JLars36920
(none) ITABAJARA DA SILVA VAZ JUNIOR
isotec ITABAJARA DA SILVA VAZ JUNIOR
Capsaicin Jack M. Bernstein, M.D.
luminous pen instead of radioactivity Bill Jack
SSCP gels Donna Jensen
RNA hyb solns? JoeJoe
Thermocyler runs JoeJoe
formaldehyde deact. w/ glycine? JoeJoe
teflon tape JoeJoe
XTT assay Geir Johannessen
DNA from formaldehyde preserved specimens JohnS52765
TaKaRa Single-Site Amplification David Johnson
Capillary Electrophoresis Todd Johnson
Capillary Electrophoresis equipment Todd Johnson
DNA FROM HAIR Todd Johnson
native gel Todd Johnson
teflon tape (Dave Johnston)
Hybaid products (Dave Johnston)
Long gel dryers (Dave Johnston)
Olomoucine, a cdc2 Protein Kinase inhibitor? Mandy Johnstone
ss DNA expression in Mammalian cells Dr. M.D. Jones
Question on generating nicked circular DNA using EthBr and EcoRI Zophonias O. Jonsson
subscription JOHN A. KORTE
hybridization bags Ron Kagan
Epitopes for prokaryotic proteins Kah-Tong, Seow
How do you measure rat's blood pressure??? Stefan Kahlert
Test,please ignore Fredrik Kamme
Electron microscopy of Plasmid Ali Karami
Denatured DNA and fixation Frank Karlsen
Fixation of DNA close to protein Frank Karlsen
south-western blot Dr Simminder Kaur
pRc/CMV containing lacZ Yoshiki Kawabe
PCR from fungal colonies J Kennedy
ANTIBIOTIC STORAGE Martin Kennedy
Biotrace membrane - Gelman Martin Kennedy
gene targeting -- homologous recombination -- why not liposomes? Martin Kennedy
Does no polyadenylation site mean no polyadenylation? Martin Kennedy
Bulk prep problems Martin Kennedy
non-rad colony screening Roger Kennett
Why MOPS for RNA gels? Oleh Kevalchuke
RNA elution from agarose Oleh Kevalchuke
KLENTAQ manufacturer In C. Kim
Designing Ideal PCR Primers In C. Kim
Dimerization of plasmid in mini-preped sample? Jinsuk Kim
Advice on Film Recorders Mike Kitamura
News Posting..eg/ Klaus.Matthaei at ANU.EDU.AU
EXONUCLEASE III Klaus.Matthaei at ANU.EDU.AU
Riboprobe background hybridisation Klaus.Matthaei at ANU.EDU.AU
Long gel dryers Klaus.Matthaei at ANU.EDU.AU
Riboprobe background vector Klaus.Matthaei at ANU.EDU.AU
Maybe a stupid PCR question & a ETBr question Klaus.Matthaei at ANU.EDU.AU
luminous pen instead of radioactivity Dorothy Klein
BPDE-DNA Hannes Klump
DISCUSSION - plasmid piracy = software piracy ? Alex Knight
ECL for colony hybridization Rinkei Ko
Cheaper electroporation cuvettes? Eric Kofoid
How do you measure rat's blood pressure??? Steve Konings
what is the best miniprep Sasha Kraev
Dye to colour DNA samples fro PCR Sasha Kraev
SDS problem Sasha Kraev
cycseq question/YAC sequencing Sasha Kraev
Phage plaque PCR Sasha Kraev
direct seq of PCR products Sasha Kraev
Re. direct seq of PCR products/Epicentre Kits Sasha Kraev
Long Ranger Gels Sasha Kraev
Long Gel Dryers Sasha Kraev
long run sequencing Sasha Kraev
plasmid dimerization Sasha Kraev
hot start in PCR(cycle seq) Sasha Kraev
NaAc pptn Jeff Kramer
nucleobond AX Jeff Kramer
downward DNA transfer? Cornelius Krasel
receptor cloning Cornelius Krasel
In search of the "Holy manual" Cornelius Krasel
Promega on the Internet Cornelius Krasel
PacI weirdness Cornelius Krasel
Sequencing vector Needed ! Cornelius Krasel
Codon Usage and Expression in E. coli Cornelius Krasel
Baculoviral Info: Cornelius Krasel
Extraction of DNA from agarose gels Cornelius Krasel
synthetic proteins Cornelius Krasel
Free BioTechniques Past Paper Database Cornelius Krasel
ammonium sulfate precipitation of proteins Cornelius Krasel
RNA structure graphics software Cornelius Krasel
Adding membrane signal to cytoplasmic protein Cornelius Krasel
curing bacteria of plasmids Candace Krepel
Motifs for txn factors? Dubear Kroening
DNA/phenol problem Stuart Kuhstoss
PCR gene splicing: help! Klas Kullander
Cheaper electroporation cuvettes? Klas Kullander
pRc/CMV containing lacZ Klas Kullander
NIH-3T3 drug test Klas Kullander
address for Dynatech? Klas Kullander
UV-crosslinking of DNA-protien complex L.Y.Chau
measuring peptide concentration-exactly! STRUCTURAL NEUROBIOLOGY LAB
Small RNA and Polysome isolation LACYP at wsuvm1.csc.wsu.edu
downward DNA transfer? LEES at SASK.USASK.CA
Marker for Plant? LEES at SASK.USASK.CA
DNA* LEES at SASK.USASK.CA
Kunkel mutagenesis- Help needed LEMAILLET
TOMATO cDNA library LEMAILLET
Cleavage with NTCB Fabrice PARMENTIER LESAGE
non-radioactive DNA sequencing LKM100F at ODUVM.BITNET
Rf: Aggregation of membrane proteins LOGAND
membrane protein aggregation LOGAND
Rf: In search of the "Holy manual" LOGAND
Rf: Antibodies to KDEL or kXXkx peptides LOGAND
anti-KDEL and anti-KxxKx antibodies LOGAND
Macherey-Nagel Nucleobond for Seq.? LOGAND
Rf: Strength of 4ml Spec. Cuvettes LOGAND
Antibody against neomycin phosphotransfera Dan Rawlins Lab
is there an index, restr. enz. sources??? Dana Macelis-Roberts Lab
microwave miniprep of plasmids S.A. Benson Lab
Help:microwave miniprep of plasmids S.A. Benson Lab
Mouse H-2D or K genomic clone Carlisle Landel
Enzymes in KGB? Carlisle Landel
retinol, cholecalciferol, vitamin B12 Stephen R. Lasky
Reticulocyte Lysate Stephen R. Lasky
Free BioTechniques Past Paper Database Carl Lawyer
DNA* Carl Lawyer
Sequencing YACS Martin Leach
Extraction of DNA from agarose gels Martin Leach
pRc/CMV containing lacZ Martin Leach
antibody to luciferase commercially available? Martin Leach
antibody to luciferase commercially available? Martin Leach
Transfection of neuronal cell lines??? Martin Leach
cDNA library from wheat and soybean Martin Leach
Where to buy genomic libraries? Martin Leach
Inverse PCR Martin Leach
internet for the mol. biologist Martin Leach
internet for the mol. biologist Martin Leach
Contamination in Nested PCR Martin Leach
Assymetric PCR references Martin Leach
CAT assays Martin Leach
DISCUSSION - plasmid piracy = software piracy ? Martin Leach
TA Vector Martin Leach
random priming Martin Leach
Luciferase Problems? Martin Leach
Need help with Eczema Harold O. Leduc
Help me!! - prmoter inversion vector Lee, Jae-Hyun
Free BioTechniques Past Paper Database Lee, Jae-Hyun
Address or phone needed Donna Leeper
microwave miniprep of plasmids K. Legate
FILTRATION K. Legate
FILTRATION (K.LEGATE) K. Legate
HA epitope tag problems K. Legate
Active Rb protein Ab Brett Levay-Young
help for oligo hybridization Limin Li
screening plasmid libraries Yuanhao Li
beta-scanner Walt Lilly
retroviral DNA preps Lorena C. Lim
Companies on the net Jonas Lindeberg
Kunkel mutagenesis- Help needed Jonas Lindeberg
Qick nuclear extract preparation ? Jonas Lindeberg
Long gel dryers Jonas Lindeberg
XL-PCR ? Jonas Lindeberg
Sal I cloning problems Jonas Lindeberg
5'- 3' Lauri Lintott
formal/agarose gels Lauri Lintott
subcloning lamda library Lauri Lintott
cloning-lambda-inserts Lauri Lintott
GeneClean versus Quiagen Joseph Lipsick
luminous pen instead of radioactivity Joseph Lipsick
Protein FPLC (HPLC) Systems Jim Litts
non-rad colony screening LizH277522
oligoscreening ? Joan Rigau Lloveras
Fluorometer Muhammad Lodhi
Circular Dichroism and aggregates Mark Jason Logan
Oligo dT for RT? Elwyn Loh
DEPC treated water in PCR John W. Longshore
PCR of GC-rich genes John W. Longshore
HELP!!! Electroporation and PEG Alfredo Lopez
More PEG and Electrophoration Alfredo Lopez
GST fusion: Solutions and more questions Ann Loraine
Cheaper electroporation cuvettes? Heather H Lord
Quantitation of Westerns Jeanne Loring
temperature-dependence of DNA-gyrase Dmitry A. Los
What's in Megascript buffer ? Patric Lundberg
EcR plasmid from Hogness at Stanford, CA Patric Lundberg
Quick response from Hogness lab! Patric Lundberg
urea/SDS, guanidine/SDS PAGE Patric Lundberg
Promega on the Internet Peter Lundberg
How good is the Eagle Eye II ? Alternatives ? Alan Lyons
protease cleaving Ser-Ala M.A.Schmidt
ANTIBIOTIC STORAGE MAD, MAD, MAD SCIENTIST
wizard prep of DNA MAD, MAD, MAD SCIENTIST
DNA/phenol problem MAD, MAD, MAD SCIENTIST
Bioventures DNA isolation Kit MAD, MAD, MAD SCIENTIST
Long gel dryers MBDMITRY at CCVAX.SINICA.EDU.TW
10 mers DAVID MITCHELL
EtBr-again KARI KAMMIOVIRTA MMKAB
Were we can buy cheapest DNA primers? KIMMO KOIVU MMKAT_OPISK
Were we can buy cheapest DNA primers? KIMMO KOIVU MMKAT_OPISK
DEPC treated water in PCR AARON J. MOE
Quickprep mRNA isolation from Pharmacia MONSTER
Why dATP ? MONSTER
Which Kit? MONSTER
direct sequencing of PCR products MONTESCLAROS,LUZ,MISS
homologous transfection system MUCHA at edv1.boku.ac.at
What's in Megascript buffer ? MUNGO at UVVM.UVIC.CA
Formiminoglutamate MURLEY,LAURA LEA,MS
silver staining of DNA NEAL MUSTO
storage of RNA NEAL MUSTO
PCRE:PCR PRIMER DESIGN NEAL MUSTO
PCR crash Emma Macfarlane
exonuclease I Walter Malangone
35 mm slides of gels - help Malcolm Moos Jr., M.D., Ph.D.
5' RACE Malcolm Moos Jr., M.D., Ph.D.
Sequencing insert from Lambda gt10 vector- HELP! Malcolm Moos Jr., M.D., Ph.D.
Why MOPS for RNA gels? Malcolm Moos Jr., M.D., Ph.D.
Buffer for plaque phage lysis --> PCR ? Malcolm Moos Jr., M.D., Ph.D.
Operon (?) Inc. - need Address etc... Mandalaparthy
need protocol for pcr of phage from plates. Mandalaparthy
need protocol for pcr:gt10vector, frm plaques. Mandalaparthy
tritium-labelled proteins: best detection method Jonathan B. Marder
Nonrad. ATP synthase assays? Ferenc Marincs
Nonrad. ATP synthase assays? Ferenc Marincs
simple protocol for colony PCR Ferenc Marincs
RNA from bacteria Ferenc Marincs
direct sequencing of PCR products Steve Marsh
GC-rich region Richard Kenneth Bruce Martel
Long gel dryers Richard Kenneth Bruce Martel
cloning-lambda libraries Richard Kenneth Bruce Martel
cold SSCP Daoust Martin
freeze-sqeeze Daoust Martin
book Daoust Martin
GC-rich region Daoust Martin
Inverse PCR Viraj Master
Idaho Technology Air Thermo-Cycler... Any good? Viraj Master
Best method for blunting ds DNA? David Matthews
Spectrophotometer Help Andrew McArthur
non-radioactive southerns C. McConville
QUESTIONS ON PCR CONDITIONS W/ DEGENERATE PRIMERS Stephen M. McCrain
Help-plasmid recovery from dead plate! Tara McCready
Taq slippage in PCR John H McDonald
Looking for a plasmid John McDougall
>>>Help about formal/agarose gel <<< Steve McKinney
Best strategy from peptide sequences to cDNA clone? Steve McKinney
Does no polyadenylation site mean no polyadenylation? Steve McKinney
Contamination in Nested PCR Patty McManus
Reverse northerns,cDNA not hot enough. Robert McMaster
Dye to colour DNA samples for PCR Ciaran Meghen
Optimisation of Hair Extraction Protocol Ciaran Meghen
ANCIENT DNA Ciaran Meghen
Inosine-containing oligos Grace Mendoza
RNA isolation out of OCT embedded sections? Harry Menegay
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" David J. Meyer
Buffering sat'd NaI sol'ns David J. Meyer
Bind-silane removal David J. Meyer
B.M.s 5 minute ligations? David Micklem
Single stranded DNA restriction?????? Mike Powell, PhD
temperature-dependence of DNA-gyrase Steve Minchin
Problem with Native PAGE Ding Ming
Detection of "Invisible" Proteins??? Ding Ming
Lambda clone isoln - Lambda fix Robert E Minto
RT-PCR question Sean David Moore
PCR problems Sean David Moore
stimulation of BT-20's & MCF-7 cells Philip Joseph Moos
PCR gene splicing: help! Kevin Morano
PCR crash Kevin Morano
immunoprecipitation Ralf Morgenstern
storing protein samples? **** Shahram Mori
secondary screening after differential display Shahram Mori
cDNA Challenge************* Shahram Mori
RT-PCR question Shahram Mori
cDNA Challenge************* Shahram Mori
RT-PCR question Shahram Mori
Single stranded DNA restriction?????? Shahram Mori
ANCHORED-PCR Shahram Mori
Capsaicin Mike Morris
Optimisation of Hair Extraction Protocol Mike Morris
NuSieve Agarose Mike Morris
Q: Human cDNA libraries? Mike Morris
Test Message - Don't read! Peter Mulderry
Smallest Cloning Vector and Selectable Marker ... Scott B. Mulrooney
cDNA library screens via PCR Sandra Munro
ECL for colony hybridization Pete Muriana
suggestion: include email address in publications Pete Muriana
curing bacteria of plasmids Pete Muriana
Why dATP ? Pete M. Muriana
PHAGE DNA KITS? T. J. Murphy
Best method for blunting ds DNA? Bernard Murray
Best method of blunting DNA: SUMMARY and THANKS Bernard Murray
>>>Help about formal/agarose gel <<< Bernard Murray
Detection of "Invisible" Proteins??? Bernard Murray
Long-Range PCR Mwatson at uniwa.uwa.edu.au
Beteine and inclusion bodies Rik Myers
Shotgun sequencing assembly Peter Myler
PCgene program Peter Myler
DNA* Peter Myler
detection of 5-methyl cytosine (bisulfite method) Onno Myriam
detection of 5-methyl cytosine (bisulfite method) Onno Myriam
detection of 5-methyl cytosine (third question)) Onno Myriam
Beteine and inclusion bodies Daniel Mytelka
ABRF network NISSON at BIOTECHNET.COM
Luciferase as internal control John J Naleway
Promega on the Internet: Biotechnet John J Naleway
Companies on the Net John J Naleway
HRP substrate that fluoresces John J Naleway
Luminescent vs fluorescent ELISA John J Naleway
Marker for Plant? John J Naleway
staining for beta-galactosidase John J Naleway
Kits for Subtraction Libraries? John Nash
Which PCR machine? John Nelson
Capillary Electrophoresis Robert Nelson
Need address for Molecular Probes Inc. Robert Nelson
DNA Amplification Market Robert Nelson
RNA Isolation Robert Nelson
Were we can buy cheapest DNA primers? Robert Nelson
PCR crash Robert Nelson
Companies on the net Rick Neubig
looking for calpain antibody Robert Neumar
RT-PCR question Gerri Newfry
PCgene program Gerri Newfry
minipreps for sequencing Thomas Newman
virus receptor research--Help I-shiou Ng
Pharmacia GeneQuant DNA calculator opinions wanted Prenom Nom
In search of the "Holy manual" Frank Norman
Promega on the Internet: Biotechnet Frank Norman
cDNA Challenge************* Andy Law Big Nose
subscripte bionet.molbio.methds-reagnts Danielle Nouaud
PCR journal Kevin O'Donnell
Diagnostics Prototype Newsgroup Kevin O'Donnell
Adding membrane signal to cytoplasmic protein ORSULIC at UNCVX1.OIT.UNC.EDU
dig labelled Riboprobe whole mount insitu? Dan Oerther
Ambion RPA II Kit?? Guilherme Co. Oliveira
kinases Torben Osterlund
Detection of "Invisible" Proteins??? Torben Osterlund
sequence of pSP64(polyA) - Promega Francis Ouellette
internet for the mol. biologist Francis Ouellette
internet for the mol. biologist Francis Ouellette
internet for the mol. biologist Francis Ouellette
>>>>URGENT : IN VITRO TRANSCRIPTION <<<<< Jim Owens
Long gel dryers Jim Owens
DNA/phenol problem Jim Owens
chemistry of Qiaex beads & DNA interaction?? PRAMATHESH PATEL
clean GST antibodies source????? PRAMATHESH PATEL
Quickprep mRNA isolation from Pharmacia PEDP043 at uabdpo.dpo.uab.edu
? Supplier of mRNA Diff. display kit RICHARD P. PHIPPS
HA epitope tag problems PJOB
How good is the Eagle Eye II ? Alternatives ? PMacklon
Far-Western help POLAKBM at WPOGATE.SLU.EDU
Prokaryotic fusion protein systems POLAKBM at WPOGATE.SLU.EDU
Viral Cell Packaging line GP+E86 POLAKBM at WPOGATE.SLU.EDU
what's the best way to clone PCR product POPOVA
single-copy vectors POPOVA
Oligo dT for RT? POPOVA
Coupling IgG via sugar residues POPOVA
Problems w/ native PAGE POPOVA
Method for Touchdown PCR Paiks
Ghosts and membrane fluidity Jan Palaty
ECL direct Dieter Palmer
ECL direct Dieter Palmer
XTT assay Dieter Palmer
simple protocol for colony PCR Anthony Palombella
hybridization bags Dave Pataky
DIG-labelled antisense for RNAse protection? Jose Nieto Path
Coupling IgG via sugar residues Dave Pearton
Spin columns for DNA band excision? Mario de la Pena
Need reliable method for RNA extraction from lymphocytes Howard T. Petrie
Need reliable method for RNA extraction from lymphocytes Howard T. Petrie
Spin columns for DNA band excision? Howard T. Petrie
Requesting pGEM-luc and pSL301 daren Grossman - Pharmacology
hybridization bags Genetic Phlegm
receptor cloning T. S. Pillay
clean GST antibodies source????? T. S. Pillay
anti-phosphoserine/threonine Ab? T. S. Pillay
anti-phosphoserine/threonine Ab? T. S. Pillay
? Supplier of mRNA Diff. display kit T. S. Pillay
Tyrosine kinases in bacteria T. S. Pillay
GST T. S. Pillay
Immobilon CD membranes- do they work? T. S. Pillay
Extraction of DNA from Agarose Gel T. S. Pillay
beta-globin antibodies? Geoff Pinchbeck
How annoying (was Re: RT-PCR question) Michael Poidinger
Cutting Edge (Was Re: Recent DNA and RNA isolation methods wanted) Michael Poidinger
posting to bionet Michael Poidinger
Transfection of neuronal cell lines??? Michael Poidinger
staining for beta-galactosidase Michael Poidinger
Sequencing with hexamers (was degenerate primers) Richard T. Pon
Companies technical information supply online Bert Popping
Yeast cDNA library kit Bert Popping
Storage of RNA Rosamund Eira Powles
Electroelution and deglycosylation Prasad, Krishna
is there an index, restr. enz. sources??? Robert Preston
Thermocyler runs Robert Preston
Fax of Genelabs Technologies Otto Pritsch
LacZ in eukaryot. promoter studies Przemko
Question on generating nicked circular DNA using EthBr and EcoRI Rock Pulak
Kits for Subtraction Libraries? Kevin Pumiglia
Adding membrane signal to cytoplasmic protein Kevin Pumiglia
Pharmacia GeneQuant DNA calculator opinions wanted PENNY RIGGS
anti-Taq polymerase antibodies for hotstart RMARTINQFD at BIOTECHNET.COM
Need Advice on Film Recorders RNeubig at umich.edu
hemagglutinin epitope for Westerns ROSS at SCI.WFEB.EDU
Aggregation of membrane proteins Dayanidhi Raman
Info. on refrigerated microfuge Ramanujam Raman
Stability of Heparin Ramanujam Raman
rat cDNA library Mukul Ranjan
HRP substrate that fluoresces Rascals
Extraction of DNA from Agarose Gel Laurence T. Reiter
Transient transfection assays. Laurence T. Reiter
PFGE and YACs Department of Research
direct sequencing of PCR products Department of Research
Sequencing with degenerate primers RichWalko
35 mm slides of gels - help Richard
Promega on the Internet Richard
Tritium screen 0 : EnHance 1 Richard
Tritium screens Richard
ss DNA expression in Mammalian cells Richard
Artifacts in silver staining Richard
Homebrew saline for Contact Lenses Brad Riesenberger
Membrane protein fusion vectors? Know of any? Paul Riggs
sequence of pSP64(polyA) - Promega Robert.Coelen at jcu.edu.au
simple protocol for colony PCR Robert.Coelen at jcu.edu.au
RTPCR/degenerate primers with inosine? Robert.Coelen at jcu.edu.au
Summary: simple protocol for colony PCR Robert.Coelen at jcu.edu.au
EXONUCLEASE III Michael Austin Roberts
Sequencing YACS Bruce Roe
Cycle Sequencing Question Bruce Roe
help for oligo hybridization Gabriel Romero
Baculoviral Info: Brad Romney
PFGE and YACs Elizabeth Rosenberg
S1 Nuclease kits Traci Roth
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" Traci Roth
CAT assays Traci Roth
Homebrew saline for Contact Lenses Robert Rumpf
In search of the "Holy manual" SPAGNOL at PLAUTO.CSATA.IT
Phosphoimagers vs Instantimager Roland J Saldanha
Phosphoimagers vs Instantimager Roland J Saldanha
E. coli lacZ complementation Roland J Saldanha
oligonucleotide synthesizers Roland J Saldanha
what's the best way to clone PCR product Klaus Salger
small DNA fragment recovery Klaus Salger
non-isotopic gel shift assays Klaus Salger
Best method for blunting ds DNA? Klaus Salger
Glowing BLUE DNA Gels Klaus Salger
is there an index, restr. enz. sources??? Klaus Salger
Codon preferences of differrent organisms Klaus Salger
----URGENT : RNAase Free Water ?---- Klaus Salger
teflon tape Klaus Salger
DIG system for Northern blotting Klaus Salger
Long gel dryers Klaus Salger
EtBr-again Klaus Salger
Enzymes in KGB? Klaus Salger
request advice on mRNA preparation Klaus Salger
Wanted - buffer systems for pH range 4 to 9 in cell culture expt. Mr. P.A. Sansom
homocystamine (aminopropanethiol) Santhosh
RT-PCR question Adi Santoso
pIN-III-ompA-hind Dr. Jon R Sayers
Plasmid collections-where? Dr. Jon R Sayers
luminous pen instead of radioactivity Leo Schalkwyk
Codon Usage and Expression in E. coli Rebecca Schall
Bioventures DNA isolation Kit Rebecca Schall
Transfection of oesophageal (squamous cell)carcinoma cell line Irene Schauer
DNA-affinity columns Alan Schoenfeld
dig labelled Riboprobe whole mount insitu? P. Jones/ P. Scotting
Wanted Japan/uk fellowship candidate P. Jones/ P. Scotting
PC-based plasmid map programs Hank Seifert
PCgene program Hank Seifert
ATCC site Loryn Sellner
Homebrew saline for Contact Lenses Loryn Sellner
Homebrew saline for Contact Lenses Loryn Sellner
RNA from paraffin embedded tissue Loryn Sellner
ANNOUNCING: ALF DNA Sequencer Discussions in bionet.genome.chromosomes Jackson Shea
Sequencing problem, HELP!! Glen Shearer
RNA hyb solns? Glen Shearer
Why MOPS for RNA gels? Glen Shearer
PHAGE DNA KITS? Glen Shearer
long ranger gels Glen Shearer
Ziplox - expressible? J Shepherd
electroporation Yoshiyuki Shibata
Maybe a stupid PCR ques Margaret Shirra
GeneClean versus Quiagen Michael Sidler
half life of GUS, luciferase and other reporters? Jeffrey Alan Silverman
MEK phosphorylation Jeffrey Alan Silverman
storing protein samples? **** Robert Slany
Promega on the Internet Dave Smith
Sequencing with degenerate primers Dave Smith
pRc/CMV containing lacZ Dave Smith
>>>Help about formal/agarose gel <<< S L Smithson
anti-phosphoserine/threonine Ab? Jae-Won Soh
keratinase / alkaline protease Tony Soltis
HA epitope tag problems Anne Spang
Western Using Monoclonals Anne Spang
test Charlie Spillane
Which PCR machine? Darren Stauth
Maybe a stupid PCR question & a ETBr question Darren Stauth
DNA/phenol problem Darren Stauth
Anyone using "hot start" cycle sequencing? Darren Stauth
cDNA kits? Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
ANTIBIOTIC STORAGE Stephen_W._Mamber_at_~PRIWFB05 at ccmail.bms.com
What's the best mini-prep? B Stickney
Detection of rRNA methylation B Stickney
Tris and DEPC B Stickney
Info. on refrigerated microfuge B Stickney
Storage of RNA B Stickney
PostDoc Molecular Virology New Jersey (job) Vic Stollar
Kits for Subtraction Libraries? John Storring
Volatile buffers info (TEAB; TEAA) Gregory Stuart
promoter search Satyam Subrahmanyam
Geiger Counter Satyam Subrahmanyam
(none) Zalman L. Suldan
electroporation cuvettes Zalman L. Suldan
Extraction of DNA from agarose gels Clemens Suter-Crazzolara
receptor cloning Clemens Suter-Crazzolara
protein precipitates:HELP ! Clemens Suter-Crazzolara
protein precipitates:HELP ! Clemens Suter-Crazzolara
SDS Problem Clemens Suter-Crazzolara
protein precipitates:HELP ! Clemens Suter-Crazzolara
teflon tape Clemens Suter-Crazzolara
Does no polyadenylation site mean no polyadenylation? Clemens Suter-Crazzolara
PCR crash Clemens Suter-Crazzolara
Ab for 6XHis tag?? Clemens Suter-Crazzolara
Smallest Cloning Vector and Selectable Marker ... Clemens Suter-Crazzolara
Rotofor preparativ IEF Jan Svensson
What is the perfect PCR? Agnieszka Szczepek
yeast genomic library PAUL TASLIMI
Yeast genomic Library PAUL TASLIMI
Why MOPS for RNA gels? NICHOLAS THEODORAKIS
VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY NICHOLAS THEODORAKIS
>>>Help about formal/agarose gel <<< NICHOLAS THEODORAKIS
Detection of "Invisible" Proteins??? NICHOLAS THEODORAKIS
Dynatech address Tahilyamani at pahkr6.Syntex.Com
urea/SDS, guanidine/SDS PAGE Song Tan
Sal I cloning problems Lisa Taylor
Syringe pump WANTED Lorne Taylor
Anti-LexA protein antibodies?? Dennis Templeton
long ranger gels Peter Thorén
Idaho Technology Air Thermo-Cycler... Any good? Peter Thorén
Clontech Ligation Express Richard T. Timmer
Westerns to detect HA Tag? ToddS41
Promega on the Internet Anthony Tomlinson
q Bianca L Torrez
ANCIENT DNA Chris Troy
ANCIENT DNA Chris Troy
Corvas International David Tuminaro
(none) Bruce J. Turner
oligo hybridization Bruce J. Turner
GST fusion: Solution and more questions Rodney Tweten
T4 ENDONUCLEASE VII URA1335 at ARTHUR.CITI2.FR
T4 ENDONUCLEASE VII ??? URA1335 at ARTHUR.CITI2.FR
hybridization bags Udo Schlosser, D1.4.03, Tel.: 6253
GAL4 fusion/expression vectors ? Peter Uetz
Sequencing with degenerate primers Chris Upton
Home made glass FOG/MIST request Chris Upton
DISCUSSION - plasmid piracy = software piracy ? Chris Upton
SS amplification woes VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
enhancer dependent color Varyc
HIV-1 panel Varyc
Shotgun sequencing assembly Varyc
Alk phos conjugation Varyc
Detection of "Invisible" Proteins??? - Reply Varyc
error free proteins Varyc
mycobact databases Varyc
minipreps for sequencing Varyc
Geiger Counter Paul A. Vescio
teflon tape Paul A. Vescio
problem with native PAGE Nora Vig
Help needed with alkaline phosphatase conjugation to oligonucleotides Dominic Voon
direct sequencing of PCR products Karl Voss
Need reliable method for RNA extraction from lymphocytes Ben Vowels
minipreps for sequencing Stephane Vuilleumier
promega wizard kits and the 373 WAS
Promega on the Internet WICKHAM,JOHN
Expression problems WICKHAM,JOHN
E. coli lacZ complementation HERBERT WYCKOFF
Pseudomonas/bacteriocins FJ van der Wal
Hair Follicle DNA Fingerprinting Shiao Y. Wang
Dye to colour DNA samples for PCR Shiao Y. Wang
Taq slippage in PCR Shiao Y. Wang
Help!!! Removing polysaccharides from DNA preps Devaka Weerakoon
HELP: NORTHWESTERN ASSAY John J Weiland
Oligonucleotides as Linkers? Weiss
Promega on the Internet Helge Weissig
Promega on the Internet Patrick Weix
Best method for blunting ds DNA? John J Welch
DNA/phenol problem Jennifer_ Welsh
ask for information Wenfu
anti-Taq polymerase antibodies for hotstart Carolyn M Wetzel
Macherey-Nagel Nucleobond for Seq.? Frank White
antibody to luciferase commercially available? Sheryl White
antibody to luciferase commercially available? Sheryl White
rBGH organism Roger Wiegand
error free proteins Roger Wiegand
Extraction of DNA from agarose gels Wiggy
DNA purification Wiggy
help: protocol for removal of dye terminators for cyc. seq. John Williford
Kunkel mutagenesis- Help needed Berthe M. Willumsen
Tritium screens / protein gels Berthe M. Willumsen
TRANSFAC database Edgar Wingender
Seeking ELISA plate reader advice Donald Chun Kit Wong
Seeking ELISA plate reader advice Donald Chun Kit Wong
ELISA microplate reader Donald Chun Kit Wong
GST Surasakdi Wongratanacheewin
kinases Jim Woodgett
anti-phosphoserine/threonine Ab? Jim Woodgett
Tyrosine kinases in bacteria Jim Woodgett
HA epitope tag problems Jim Woodgett
Pharmacia GeneQuant DNA calculator opinions wanted Robert Woodward
luminous pen instead of radioactivity Robert Woodward
Human cell transformation- SOD1 and SOD2 genes Brad Wouters
inverse PCR & the price of anchovies B.W. Wren
inverse PCR & the price of anchovies B.W. Wren
sig John Wurzelmann
protein expression - vessel size: summary XRDPROC at Gems.VCU.EDU
silica based DNA extraction Jinghai Xu
dhfr antibody Min Xu
Help! DNA insoluble in water Nanfel Xu
Problem with Native PAGE Pin Xu
Best method for blunting ds DNA? Annie Yang
qiagen column regeneration Annie Yang
Phosphoimagers vs Instantimager Ian A. York
35 mm slides of gels - help Ian A. York
GeneClean versus Quiagen Ian A. York
Help! DNA insoluble in water Ian A. York
Co-transfection methods Ian A. York
SDS Problem Lester Young
tandem signal sequences Pak-Lam Yu
tandem signal sequences Pak-Lam Yu
Need address for Molecular Probes Inc. Paquette Yves
Sequencing problem, HELP!! Zainul Zain
Methionine-free insect cell culture media Matthias Zeiner
clean GST antibodies source????? Matthias Zeiner
mutiple and faint bands in PCR Xin-Hua Zhou
UV-crosslinking of DNA-protien complex Frances Hannan Zoology
Info. on refrigerated microfuge Frances Hannan Zoology
Direct sequencing for ABI 373 a.cox at rbgkew.org.uk
VectorNT1 experiences? adler at staff.monash.edu.au
FILTRATION ae55
FILTRATION (K.LEGATE) ae55
How do you transform aorta-smooth muscle cells? aeschba at fmi.ch
Substitute for F12 K-media for HUVECs wanted aeschba at fmi.ch
Info on Dynal magnetic beads (efficiency of extraction) ajayaram at pearl.tufts.edu
oligonucleotide synthesizers altabios.internet{altabios at bham.ac.uk}
Taq pol patent update an125249 at anon.penet.fi
Taq pol patent update an125249 at anon.penet.fi
UV-crosslinking of DNA-protien complex anon
long film & cassettes for sequencing - where? anon
NaAc precipitation anon
Help! DNA insoluble in water anon
Extraction of DNA from Agarose Gel anon
help ahmet arman
PCgene program b137795 at mailserv.cuhk.hk
RoboCycler? dudy bar-zvi
Plasmid copy number control bchs1b
Dimerization of plasmid in mini-preped sample? benedik at uh.edu
Codon preferences of differrent organisms bio4 at ccnet.up.ac.za
random priming braam at BIOC.RICE.EDU
PacI weirdness brett at BORCIM.WUSTL.EDU
small DNA fragment recovery lesley brown
lexA-antibody mingzi chen
RACE, need help chien at mbcf.stjude.org
QUICKHYBE from Stratagene - opinions? steven joseph chmura
MATCHMAKER Two-Hybrid Supplement clontech!pauld at NETCOM.COM
refrigerated cent. colvin at ouvaxa.cats.ohiou.edu
DNA sequencing services cote at bscr.uga.edu
Measuring Plant Cell Death cote at bscr.uga.edu
Spin columns for DNA band excision? crawford at bscr.uga.edu
RT storage? croyleml at ucbeh.san.uc.edu
What's the best condition for transfection of most commonly used cell lines? Aurel d'etre
Desperation - how do I get off? dacoj3 at wptemp.kvl.dk
How good is the Eagle Eye II ? Alternatives ? dallas at mcvax4.d48.lilly.com
ammonium sulfate precipitation of proteins bipin dalmia
Quantitative protein determinations in plant extracts bipin dalmia
Some Receptor Theory Questions...Constitutive or not? davis at wehi.edu.au
pulse field gels shelly may deane
Phosphoimagers vs Instantimager dhanzel at delphi.com
Quantitation of Westerns dhanzel at delphi.com
non-radioactive southerns dhanzel at delphi.com
Luminescent vs fluorescent ELISA dhanzel at delphi.com
Need address for Molecular Probes Inc. dhanzel at delphi.com
luminous pen instead of radioactivity dkatz at doane.edu
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" dkim at unm.edu
FILTRATION (Gelman Sciences) donnas at gelman.com
PCR of GC-rich genes doug_mckenzie at stratagene.com
ECL for colony hybridization dpetersn at unixg.ubc.ca
direct seq pcr w/abi eckloff at rcf.mayo.edu
Hot phenol prep, genomic DNA enof
Hot phenol prep, genomic DNA enof
PCR from fungal colonies enof
ammonium sulfate precipitation of proteins larson eric
Sequencing vector Needed ! f.r.byrne
Cancer Treatment Groups feco1104 at altair.selu.edu
cDNA Challenge************* douglas l feinstein
RT-PCR question douglas l feinstein
Buffer for plaque phage lysis --> PCR ? fgrun
PC-based plasmid map programs nicola fidler
Glowing BLUE DNA Gels fnord
Homemade GeneClean kits ??? fnord
Vistra frits at hubrecht.nl
Extraction of DNA from agarose gels futers at biovax.leeds.ac.uk
DNA mis-match analysis futers at biovax.leeds.ac.uk
pcDNA3 with Wizard g_watson at icrf.icnet.uk
extraction of DNA from agarose gels g_watson at icrf.icnet.uk
pSV2dhfr seqeunce anyone? g_watson at icrf.icnet.uk
pRc/CMV containing LacZ g_watson at icrf.icnet.uk
ss DNA expression in Mammalian cells g_wenck at spike.psc.scarolina.edu
teflon tape gc
urea/SDS, guanidine/SDS PAGE gflentke.internet{gflentke at facstaff.wisc.edu}
urea/SDS, guanidine/SDS PAGE gflentke at facstaff.wisc.edu
Detection of "Invisible" Proteins??? ggordon at opal.tufts.edu
wizard prep of DNA gibbs at sbsu1.auckland.ac.nz
temperature-dependence of DNA-gyrase christophe gilbert
Sequencing problem, HELP!! gli at UCDAVIS.EDU
SSCP analysis protocol gli at UCDAVIS.EDU
cold SSCP gli at UCDAVIS.EDU
subscribe gr8bn at cc.usu.edu
HELP: NORTHWESTERN ASSAY gr8bn at cc.usu.edu
long ranger gels gustilo at pobox.upenn.edu
Anyone using "hot start" cycle sequencing? gustilo at pobox.upenn.edu
Need Advice on Film Recorders gustilo at pobox.upenn.edu
Plasmid copy number jim hartley
Plasmid copy number control jim hartley
CAT assays henderson
oligonucleotide synthesizers hl15 at hubrecht.nl
half life of GUS, luciferase and other reporters? hraaxl at marc.cri.nz
southern and northern on the same blot hrichter at ulys.unil.ch
GC-rich region hschweiz
use of sodium salicylate prior to autoradiography hschweiz
Canadian Novagen Users intermed at inforamp.net
Amplification of pACYC184 plasmid jalexand at crihan.fr
txn factor consensus seq. database?? jboatri.internet{jboatri at eagle.cc.emory.edu}
KLENTAQ manufacturer jchurch at kean.ucs.mun.ca
Which PCR machine? jfh
TIGR or HGS address? jfh
test JPF jfield
Long range Genomic PCR jlanders
Bioscience Archives? jnewton at molbiol.ox.ac.uk
TA Vector jnewton at molbiol.ox.ac.uk
KLENTAQ manufacturer kamin johnson
silica based DNA extraction jxu.internet{jxu at MIT.EDU}
separating large DNA fragments-info needed kannan at kuhub.cc.ukans.edu
Radiographic Duplicating Unit kramerse at wmvx.lvs.dupont.com
oligos kreuzer at bkfug.kfunigraz.ac.at
Smallest Cloning Vector and Selectable Marker ... Allin Verreault in Stillman lab
Extraction of DNA from agarose gels leshof at delphi.com
Seeking ELISA plate reader advice littgj at wmvx.lvs.dupont.com
HRP substrate that fluoresces littgj at wmvx.lvs.dupont.com
Luminescent vs fluorescent ELISA littgj at wmvx.lvs.dupont.com
qiagen column regeneration elias lolis
Returned mail: User unknown lw75
synthetic proteins lw75
small DNA fragment recovery m-brazil at nimr.mrc.ac.uk
Phosphoimagers vs Instantimager macIIci
Minipreps for ABI sequencer ???? maria
SDS Problem john markwell
Baculoviral Info: stephen marshalko
Ecoli periplasmic secretion vectors? mbsay at seqnet.dl.ac.uk
How good is the Eagle Eye II ? Alternatives ? mcgeed at HAL.HAHNEMANN.EDU
Electroporation cuvettes mcgeed at HAL.HAHNEMANN.EDU
Electroporation cuvettes mcgeed at HAL.HAHNEMANN.EDU
Electroporation cuvettes mcgeed at HAL.HAHNEMANN.EDU
Capsaicin mdaily at delphi.com
Maybe a stupid PCR question & a ETBr question mdcabl at cc.newcastle.edu.au
baculovirus and receptors megVx
antibody database on the Net megVx
Announcement: 2 Hybrid System Review Available on MGH Gopher Server mendelsohn at Frodo.MGH.Harvard.EDU
Genesis Pharmaceuticals (Cambridge, MA) -- Combinatorial Chemistry ??? merlin
suggestion: include email address in publications merlin
gene targeting -- homologous recombination -- why not liposomes? merlin
Capsaicin mhughes at mbcf.stjude.org
Long gel dryers mhughes at mbcf.stjude.org
Adding membrane signal to cytoplasmic protein mhughes at mbcf.stjude.org
E. coli lacZ complementation mike
MSE Centrifuges (Help!) mlrjsg at dct.ac.uk
PCR problems mwatson at uniwa.uwa.edu.au
Promega on the Internet robert nusbickel
chaps nussbaum at neurochem.u-strasbg.fr
HELP: NEEDS A PIG rDNA CLONE opp at icbr
SSCP analysis protocol orbashu at ubvms.cc.buffalo.edu
Idaho Technology RAPD analysis palasj9579 at cobra.uni.edu
cold SSCP parkef at delphi.com
Need address for Molecular Probes Inc. patel_p at bms.com
hybridization bags patterson at frodo.mgh.harvard.edu
in situ PCR pattonaj%phvax.dnet at sb.com
nuclear proteins pattonaj%phvax.dnet at sb.com
kit for differential display pattonaj%phvax.dnet at sb.com
westerns using monoclonals pattonaj%phvax.dnet at sb.com
PCR gene splicing: help! pskaytes at INTNET.UPJ.COM
Epitopes for prokaryotic proteins wang qi
Western Using Monoclonals wang qi
check mail. ranga at vigyan.iisc.ernet.in
hybridization bags doug rhoads
RT-PCR question rhubner at molbiol.ox.ac.uk
Storage of RNA rhubner at molbiol.ox.ac.uk
oligo(dT) with single base at 3'-end... rhubner at molbiol.ox.ac.uk
Single stranded DNA restriction?????? rnwil003 at uctvax.uct.ac.za
Single lane/column sequencing-help rnwil003 at uctvax.uct.ac.za
Promega on the Internet robert at chironex.jcu.edu.au
Sal I cloning problems rpgrant at molbiol.ox.ac.uk
Does no polyadenylation site mean no polyadenylation? rr at BMMB01.UMESCI.MAINE.EDU
PCR of GC-rich genes sbaker at molbiol.ox.ac.uk
Summary: PCR of GC-rich genes sbaker at molbiol.ox.ac.uk
incorporating biotin-dNTPs schoenfeld at MSSCC.MED.UTAH.EDU
PCR PRIMER DESIGN schupp at nauvax.ucc.nau.edu
posting to bionet schupp at nauvax.ucc.nau.edu
DNA purification schupp at nauvax.ucc.nau.edu
EXONUCLEASE III schupp at nauvax.ucc.nau.edu
DISCUSSION - plasmid piracy = software piracy ? schupp at nauvax.ucc.nau.edu
Amplification of pACYC184 plasmid schupp at nauvax.ucc.nau.edu
Keystone Oncogenes mtg: Want Roommate seklund.internet{seklund.internet{seklund at netserv.unmc.edu}
Keystone Oncogenes mtg: Want Roommate seklund.internet{seklund at netserv.unmc.edu}
Luciferase Problems? sfraser.internet{sfraser at acs.ucalgary.ca}
pcDNA3 with Wizard lee shekter
pCI lee shekter
QUICKHYBE from Stratagene - opinions? sjchmura.internet{sjchmura at kimbark.uchicago.edu}
ANCHORED-PCR sl6dp at cc.usu.edu
non-isotopic gel shift assays slee at opal.tufts.edu
Need dephosphorylation protocol slee at opal.tufts.edu
help about formal/agarose gels slee at opal.tufts.edu
Need RNA structure program slee at opal.tufts.edu
Cheaper electroporation cuvettes? szcooley at dale.ucdavis.edu
electroporation cuvettes szcooley at dale.ucdavis.edu
KLENTAQ manufacturer tebas_p at msdisk.wustl.edu
gene-capsule thomas
Alsever's Solution henderson todd
isolation of nuclei from lymphocytes michelle tourigny
test troianovsk_s at msdisk.wustl.edu
Codon Usage and Expression in E.coli. vaughan at sci.wfeb.edu
Inverse PCR? vdrover at kean.ucs.mun.ca
Companies on the Net walling at wmvx.lvs.dupont.com
address of So. Cal forensic lab walterh.internet{walterh at u.washington.edu}
Topology of a multisubunit membrane protein wegloehner at mpimg-berlin-dahlem.mpg.de
Luciferase reporter gene with beta actin promoter x91holmen at wmich.edu
Luciferase reporter gene/beta actin promoter x91holmen at wmich.edu
problem with pUC18..help me !! Lee jang young
Overexpression with pKK223-3 !! help Lee jang young
Best strategy from peptide sequences to cDNA clone? yqhuang at iastate.edu
Electroelution and deglycosylation yqhuang at iastate.edu
PCR PRIMER DESIGN yu at ouvaxa.cats.ohiou.edu

Last message date: Mon Oct 31 23:05:18 EST 1994
Archived on: Sun Mar 6 21:26:25 EST 2005

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