Methods October 1994 Archives by author
Starting: Sat Oct 1 07:08:14 EST 1994
Ending: Mon Oct 31 23:05:18 EST 1994
Messages: 1221
test
Cheng Luo #3295
luminous pen instead of radioactivity
Stefan Gruenert (WCI)
lexA-antibody
Wanted a mentor from industry
Labuser 1
Why MOPS for RNA gels?
D.Greeve <DAEMON>
non title
額賀路嘉
(none)
Anders Bäckström
(none)
Anders Bäckström
DNA-RNA hybrid cross-linking
León Fernando García-Martínez
ab:6xHisTag
AKravetz
minipreps for sequencing
AKravetz
get rid of developer stains from clothing
AMBION at DELPHI.COM
Stool RT-PCR
Dale Yuzuki's Work Account
Long range Genomic PCR
Dave Adelson
35S-Met lifetime?
Dave Adelson
Assymetric PCR references
Ashok Aiyar
Acrylamide electrophoresis of DNA problem
Albert
chemistry of Qiaex beads & DNA interaction??
Shane Albright
protein precipitates:HELP !
Shane Albright
Need address for Molecular Probes Inc.
Stephen B. Aley
DNA sequencer
Eric C. Anderson
promega wizard kits and the 373
Eric C. Anderson
test, sorry to bother
Eric C. Anderson
Which PCR machine?
Eric C. Anderson
Best strategy from peptide sequences to cDNA clone?
Eric C. Anderson
luminous pen instead of radioactivity
Eric C. Anderson
Codon preferences of differrent organisms
Eric C. Anderson
methylmercuric hydroxide
Azlinda Bte Anwar
Phosphoimagers vs Instantimager
Tracy Aquilla
RT-PCR question
Tracy Aquilla
Why MOPS for RNA gels?
Tracy Aquilla
Phosphoimagers vs Instantimager
Tracy Aquilla
GeneClean versus Quiagen
Tracy Aquilla
EXONUCLEASE III
Tracy Aquilla
EXONUCLEASE III
Tracy Aquilla
ANCIENT DNA
Tracy Aquilla
ANCIENT DNA
Tracy Aquilla
Where to buy genomic libraries?
Tracy Aquilla
Protein Purification - Woe is me!!!
Curt Ashendel
urea/SDS, guanidine/SDS PAGE
Curt Ashendel
Why MOPS for RNA gels?
Jim Astwood
Phosphoimagers vs Instantimager
Graham Atherton
Luciferase as internal control
Graham Atherton
Disappearing retard band
Graham Atherton
Help need for Single-step RNA Ioslation by Acid Guanidinium
Thiocyanate-Phenol-Chloroform Extraction"
BAI_JN at JHUVMS.HCF.JHU.EDU
help! purifying polyclonals
BARKER, SHARON L.
staining for beta-galactosidase
BMA14432 at vax1.utulsa.edu
Homebrew saline for Contact Lenses
Shawn C. Baker
Protein FPLC (HPLC) Systems
Clifford Beall
isotec
Clifford Beall
PCR of GC-rich genes
Clifford Beall
35S labeling E coli
Michael Benedik
PCgene program
Benoit_Hebert at IAF.UQUEBEC.CA
WANTED: Old liquid scintillation counters
Ron Berry
Cosmid sequencing with the 373
Lisa Bibbs
IgM
Shaun D. Black
ATCC site
Shaun D. Black
Free sulfhydryl determination
Shaun D. Black
Ellman's Reagent/DTNB (PROTOCOL)
Shaun D. Black
Adding membrane signal to cytoplasmic protein
Shaun D. Black
48-well plate tools?
John E. Blume
Geiger Counter
Peter T. Boag
txn factor consensus seq. database??
Jeffrey H. Boatright
transcription+translation
Peta C. Bonham-Smith
c-myc expression system
Peta C. Bonham-Smith
ABI sequencing and Stratagene Taq Extender
Dirk Bornemeier
Do you have ABI Sequenase Kit problems???
Dirk Bornemeier
E. coli lacZ complementation
Chris Boyd
Coupling IgG via sugar residues
Tomas Bratt
How to Purify Glyc. Dehyd?
Dave Bremer
ANNOUNCE: USED ANALYTICAL LAB EQUIPMENT FOR SALE
John Brookes
Buffer for plaque phage lysis --> PCR ?
Stuart Brown
What is the perfect PCR?
Stuart Brown
Hair Follicle DNA Fingerprinting
Karen Browning
Info. on refrigerated microfuge
John Brunstein
Problems w/ native PAGE
Paul A Bucciaglia
Problems w/ native PAGE
Paul A Bucciaglia
Aggregation of membrane proteins
Andreas Buhr
Need address for Molecular Probes Inc.
Sebastian W. Bunka
Hybaid products
Sebastian W. Bunka
simple protocol for colony PCR
Sebastian W. Bunka
ELISA plates and protein adsorbtion
Emanuele Buratti
Optimal Conditions for Boehringer PWO enzyme in PCR
Jonathan Patrick Burke
screening plasmid libraries
Jon Burrows
In search of the "Holy manual"
Timothy Bushnell
help for oligo hybridization
Timothy Bushnell
What's the best mini-prep?
C.J. Hussussian, MD
Nonrad. ATP synthase assays?
CConley
0.02 filters
CURTIS at UTMSI.ZO.UTEXAS.EDU
35S labeling E coli
Paul Campbell
wanted: 'Simple Fool's guide to PCR'
Dave Carmean
Codon Usage and Expression in E. coli
Eric Carstens
DNA/phenol problem
Chuck Cerveny
Cheaper electroporation cuvettes?
George W Chacko
Adding membrane signal to cytoplasmic protein
George W Chacko
Protocal for DNA extension
Alex Chang
Detection of "Invisible" Proteins???
Jon Chappel
Best method for blunting ds DNA?
Tom Chappell
Detection of "Invisible" Proteins??? - Reply
Tom Chappell
Hot phenol prep, genomic DNA
David Char
long ranger gels
Mic Chaudoir
ANCIENT DNA
Mic Chaudoir
Cheaper electroporation cuvettes?
Mic Chaudoir
Homebrew saline for Contact Lenses
Mic Chaudoir
How good is the Eagle Eye II ? Alternatives ?
Mic Chaudoir
help for oligo hybridization
Mic Chaudoir
Western Using Monoclonals
Mic Chaudoir
neosystem
Michelle Chen
pGRE5.1, the inducing condtion
Shu-Chih Chen
RDA
Frank Cheng
DNA purification
Chutney
ABI sequencing and Stratagene Taq Extender
Duncan Clark
Thermus filiformis DNA polymerase
Duncan Clark
PacI weirdness
Duncan Clark
PacI weirdness
Duncan Clark
Expression problems
Duncan Clark
Codon Usage and Expression in E.coli.
Duncan Clark
E. coli lacZ complementation
Duncan Clark
Codon Usage and Expression in E.coli.
Duncan Clark
Expression problems
Duncan Clark
Bulk prep problems
Duncan Clark
DISCUSSION - plasmid piracy = software piracy ?
Duncan Clark
Transfection of oesophageal (squamous cell)carcinoma cell line
Volkert Clausen
Polysomes preparation
Michael Coady
What's in Megascript buffer ?
Michael Coady
Adding membrane signal to cytoplasmic protein
Michael Coady
35 mm slides of gels - help
Andrew Cockburn
ANTIBIOTIC STORAGE
Andrew Cockburn
Phosphoimagers vs Instantimager
Randall J. Cohrs
Companies on the Net
Randall J. Cohrs
PHAGE DNA KITS?
Randall J. Cohrs
>>>Help about formal/agarose gel <<<
Randall J. Cohrs
Info. on refrigerated microfuge
Randall J. Cohrs
teflon tape
Randall J. Cohrs
Help need for Single-step RNA Ioslation by Acid Guanidinium
Randall J. Cohrs
DISCUSSION - plasmid piracy = software piracy ?
Randall J. Cohrs
Taq left at RT - gulp! -
Susan Cole
WANTED: Old liquid scintillation counters
Matthew J. Combs
Help: Program to find quasi-restriction sites
Juan Antonio Contreras
NaAc precipitation
Michael Cooley
problem with pUC18..help me !!
Michael Cooley
Buffering sat'd NaI sol'ns
Michael Cooley
DNA/phenol problem
Michael Cooley
Fluorometer
Michael Cooley
dead cells
Seth Corey
DNA extraction: Lactob.
Heitor Luiz da C. Coutinho
protein/nucleic acid motifs
Dr. P.A. Crossey
B.M.s 5 minute ligations?
DAB
>>> Help about formal/agarose ges <<<
PHIL DANIEL
Chemical cleavage of mismatches (CCM)
Bill Oetting DGM-IHG
non-radioactive DNA sequencing
MARIA ELENA GARCIA DIAZ
Subscription
Slawek Dabrowski
RNA from blood
Richard Dana
Need help With Blood RNA prep
Richard Dana
Recent DNA and RNA isolation methods wanted
Richard Dana
cDNA library from wheat and soybean
Richard Dana
Some Receptor Theory Questions...Constitutive or not?
Dave
Type 52 Polaroid films? Who?******
David L. Haviland, Ph.D.
promega wizard kits and the 373
David L. Haviland, Ph.D.
ANTIBIOTIC STORAGE
David L. Haviland, Ph.D.
HELP!!! Electroporation and PEG
David L. Haviland, Ph.D.
Prokaryotic fusion protein systems
Simon Dawson
RT-PCR question
Simon Dawson
SDS Problem
Simon Dawson
Software to call heterozygosity from ABI output
Ron Debry
Pharmacia GeneQuant DNA calculator opinions wanted
Greg Denomme
cDNA ()TPR1) wanted
Thomas Deufel
RT-PCR in NuSieve ???
Michael Deyholos
POTATO SOMATIC MUTATIONS
Dickinson, Harry
Non-denaturing Southern Blotting
Scott J. Diede
spectrophotomer
Therese Ann Dietlin
What is the perfect PCR?
John Dixon
Enough Re: Promega on the Internet
Reinhard Doelz
Homebrew saline for Contact Lenses
Donald A. Lehn, Ph.D.
Arabidopsis cDNA
Iain Donaldson
Genomic DNA from E.coli without phenol or chloroform??
Gabriel Dorado
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et
al??
Gabriel Dorado
Which PCR machine?
Peter Doris
Dictionary of Cell Biology on WWW !
Julian Dow
Buffering sat'd NaI sol'ns
Virginia Dress
Detection of "Invisible" Proteins???
Virginia Dress
Reverse northerns,cDNA not hot enough.
Dr. YL Du
DNA from formalin preserved tissue
Thomas F Duda
Transfection of neuronal cell lines???
CARPENTER E.
Green Fluorescent Protein
CARPENTER E.
pRc/CMV containing lacZ
CARPENTER E.
Transfection of neuronal cell lines???
CARPENTER E.
PCR produced probes
TIM ELLS
Inverse PCR
TIM ELLS
Phosphoimagers vs Instantimager
Nigel Eastmond
Strength of 4ml Spec. Cuvettes
Nigel Eastmond
Test
George F Edick
Growing E. coli
George F Edick
Keystone Oncogenes mtg: Want Roommate
Steve Eklund
VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY
Bruce James Ella
Artifacts in silver staining
Bruce James Ella
VOLATILE BUFFERS
Bruce James Ella
DIG system for Northern blotting
Bruce James Ella
DIG system for Northern blotting
Elizabeth Estabrook
PCR crash
PANIAGUA SOLIS JORGE FERNANDO
protein aggregation
FORSDYKE at QUCDN.QueensU.CA
? Supplier of mRNA Diff. display kit
Patrice Ferriola
William D. Huse / Ixsys, Inc -- San Diego, CA
H. David Fischer
PFGE and YACs
Karl Fischer
Detection of "Invisible" Proteins???
Karl Fischer
RTPCR/degenerate primers with inosine?
Cynthia Fisher
exonuclease I
Parke K. Flick
Phosphoroimagers vs Instantimager Experiences Saught
Brian Foley
PCR fidelity: Calculations?
Brian Foley
txn factor consensus seq. database??
Brian Foley
Inosine-containing oligos
John E. Fox
oligonucleotide synthesizers
John E. Fox
Were we can buy cheapest DNA primers?
John E. Fox
Xenopus downtime
Sherri Fraser
NaAc precipitation
Sherri Fraser
Luminous Pens
Sherri Fraser
Luciferase Problems?
Sherri Fraser
6X myc cassette request
Lorenzo Frigerio
Luminescent vs fluorescent ELISA
James L Funderburgh
Are Ab's phosphorylated?
Michele Fuortes
Non-radioactive run on
Michele Fuortes
Idaho Technology RAPD analysis
Anthony V. Furano
antibody to luciferase commercially available?
G.E.Fazzi
Artifacts in silver staining
G.J.W.Euverink at biol.rug.nl
internet for the mol. biologist
GRIFFINHG
hybridization bags
Prasit Palitpolkanpim - GRSO
Which PCR machine?
G Gallagher
long film & cassettes for sequencing - where?
Frederick Garbrecht
PCR journal
Frederick Garbrecht
Artifacts in silver staining
Frederick Garbrecht
Long gel dryers
Frederick Garbrecht
Western Using Monoclonals
Frederick Garbrecht
QUES: Galvanic Skin Response
Todd G Gardner
Pichia single-copy gene
Nicholas Gascoigne
Transfection of CaCo-2 cells
Ane Gedde-Dahl
PC-based plasmid map programs
Peter Gegenheimer
Storage of RNA
Peter Gegenheimer
Glycogen
James Gibbs
NuSieve Agarose
Michael Gieseg
Bulk prep problems
Michael Gieseg
S1 nuclease/ exo III
Fabrice Glibert
dye in PCR
Fabrice Glibert
Maybe a stupid PCR ques
Fabrice Glibert
Which PCR machine?
Dr. Greg Gloor
PCR gene splicing: help!
Greg Gloor
cDNA kits?
Greg Gloor
? Supplier of mRNA Diff. display kit
David Glover
Homemade GeneClean kits ???
Marc Goldstein
Mouse intestine cDNA library
Richard Gomer
betagalactosidase antibodies
David Gonda
PCR PRIMERS
Efram Gonzalez
is there an index, restr. enz. sources???
Anita Gould
pCMV5 sequence ?
Roger Graham
RNA structure graphics software
Jerry Grandoni
Nonrad. ATP synthase assays?
Brian T. Greuel
ATCC site
Neil Griffin
Enzymes in KGB?
Duke Groebe
QUICKHYBE from Stratagene - opinions?
Duke Groebe
NIH-3T3 drug test
Jody Groenendyk
SUBSCIBTION
H1411Mus at HUELLA.BITNET
Ext. of DNA from agarose (which agarose)
HARDIES at THORIN.UTHSCSA.EDU
ss. DNA restriction (yes, they cut)
HARDIES at THORIN.UTHSCSA.EDU
SDS problem (for bkg, not stringency)
HARDIES at THORIN.UTHSCSA.EDU
Best strategy pep. seq. -> cDNA
HARDIES at THORIN.UTHSCSA.EDU
NaAc pptn.
HARDIES at THORIN.UTHSCSA.EDU
DNA insoluble in water
HARDIES at THORIN.UTHSCSA.EDU
Bulk prep problems (Yes, plasmids can integrate)
HARDIES at THORIN.UTHSCSA.EDU
oligos on membranes
HOWARD at MGRP.BGSM.WFU.EDU
oligo-binding membranes
HOWARD at MGRP.BGSM.WFU.EDU
sections of hard tissues falling off
HRMBDKC at HKUMD1.HKU.HK
Hybaid products
HRMBDKC at HKUMD1.HKU.HK
urea/SDS, guanidine/SDS PAGE
HSchreiner
DNA-Extr. of a Histological Cut
H Rochholz/ H Haas
teflon tape
H. Rochholz/H. Haas
PFGE and YACs
Antje Haase
(none)
S.Mitchell Halloran
urinary iodine determn; water qual. anal. with cap. elect.
S.Mitchell Halloran
Topology of a multisubunit membrane protein
Tuomas Haltia
Extraction of DNA from agarose gels
Andre Hamel
5'->3' InhibitACE
Andre Hamel
Storage of RNA
Andre Hamel
Riboprobe background hybridisation
Andre Hamel
Maybe a stupid PCR question & a ETBr question
Andre Hamel
DNA/phenol problem
Andre Hamel
Primers NOT needed for RT rx'n?!?!
Andre Hamel
YY1
Tan Shyh Han
NruI
Johannson Hans
densitometry software
Terry Hanzlik
RT-PCR question
Richard R. Hardy
Ambion RPA II Kit??
Jane Harrison
Extraction of DNA from agarose gels
Yasha Hartberg
pcDNA3 with Wizard
Yasha Hartberg
Kunkel mutagenesis- Help needed
Yasha Hartberg
Kunkel mutagenesis- Help needed
Yasha Hartberg
temperature-dependence of DNA-gyrase
Yasha Hartberg
EtBr-again
Yasha Hartberg
Problems w/ native PAGE
Yasha Hartberg
Spheroplast
Philip Heacock
Yeast gene expression in mammalian cells
Philip Heacock
Inverse PCR
Dr. C.P. Healy
Determination of genome size (Cot curves)
Dr. C.P. Healy
Inverse PCR
Dr. C.P. Healy
Chicken typeII collagen probe
Dr. C.P. Healy
Radiographic Duplicating Unit
Steven Hecht
Phosphoimagers vs Instantimager
Peter Heifetz
SDS Problem
Peter Heifetz
Phosphoimagers vs Instantimager
Peter Heifetz
Phosphoroimagers vs Instantimager Experiences Saught
Peter Heifetz
Phosphoimagers vs Instantimager
Peter Heifetz
cDNA library screens via PCR
Lynn M Heltemes
Capsaicin
Scott Selby Med Heme
EGF repeats in E.coli?
Sam Henderson
Ab for 6XHis tag??
Sam Henderson
RT-PCR question
Too Heng-Phon
Glowing BLUE DNA Gels
Paul N Hengen
Best Way Clone PCR Products
Paul N Hengen
What's the best mini-prep?
Paul N Hengen
Taq left at RT - gulp! -
Paul N Hengen
Promega on the Internet
Paul N Hengen
pcDNA3 with Wizard
Paul N Hengen
Glowing BLUE DNA Gels
Paul N Hengen
Dye to colour DNA samples for PCR
Paul N Hengen
Extraction of DNA from agarose gels
Paul N Hengen
Frequently Asked Question (FAQ) list
Paul N Hengen
freeze-sqeeze
Paul N Hengen
Green Fluorescent Protein
Paul N Hengen
Storing PK\LDH without any sulfate ?
Jean-Manuel Henry
Antibodies to KDEL or kXXkx peptides
Your Name Here
request advice on RNA storage
Robert Hewitt
request advice on mRNA preparation
Robert Hewitt
GST
Bernard Heymann
Protein Purification - Woe is me!!!
Andy Hill
address of So. Cal forensic lab
Walter Hill
CAPILLARY ELECTROPHORESIS equipment ?
Timo Hiltunen
Minipreps for ABI sequencer
Robert Hindges
Amino Acid Analysis software on www
Uwe Hobohm
luminous pen - use GLOGOS
Tony Hodge
Need reliable method for RNA extraction from lymphocytes
Chris Hodgson
Topology of a multisubunit membrane protein
Flip Hoedemaeker
ammonium sulfate precipitation of proteins
Flip Hoedemaeker
ANCIENT DNA
Guy Hoelzer
DNA Ladders for Short Fragments
Guy Hoelzer
Denatured DNA and fixation
Guy Hoelzer
cDNA Challenge*************
Annette C. Hollmann
RT-PCR question
Annette C. Hollmann
Thermocyler runs
Annette C. Hollmann
Western Using Monoclonals
Annette C. Hollmann
protease info / database
Gene Holowachuk
32P labeling tumors in vivo
Gene Holowachuk
rBGH organism
Louis Hom
Detergents and Disruption
Louis Hom
nuclear melanin
Louis Hom
Invitrogen FTP/Gopher Site
Christopher Hoover
Promega on the Internet
Christopher Hoover
Methylmercuric Hydroxide
Christopher Hoover
Dye to colour DNA samples for PCR
Robert Horton
test
David Hosfield
LacSwitch sequence
Eivind Hovig
Advse needed on DNA strand-separation
Baohua Huang
Antibody against neomycin phosphotransferase
David S. Huen
In search of the "Holy manual"
David S. Huen
Why MOPS for RNA gels?
David S. Huen
LacZ in eukaryot. promoter studies
David S. Huen
>>> Help about formal/agarose ges <<<
David S. Huen
COT 1 preparation! HELP!!!
David S. Huen
protein aggregation
Timothy R. Hughes
Homebrew saline for Contact Lenses
Eric R. Hugo
retroviral DNA preps
Olav Hungnes
35S labeling E coli
Arthur Hunt
Why MOPS for RNA gels?
Keith Hutchison
genomic dna prep
Darin Obrien IHG
Urbino Meeting...info needed
The doctor is IN.
>>>Help about formal/agarose gel <<<
CJF 90/10 INSERM
RNAse free water?
CJF 90/10 INSERM
Polysomes preparation
CJF 90/10 INSERM
GeneClean versus Quiagen
CJF 90/10 INSERM
----URGENT : RNAase Free Water ?----
CJF 90/10 INSERM
---- Polysomes preparation ----
CJF 90/10 INSERM
Green Fluorescent Protein
J.Carnwath
Free BioTechniques Past Paper Database
J.Carnwath
anti-Taq polymerase antibodies for hotstart
J.Carnwath
address for Dynatech?
J.Carnwath
Green Fluorescent Protein
J.Carnwath
HOMOLOGOUS TRANSFECTION SYSTEM
MUCHA JAN
Companies on the Net
JEMERTON at BIOTECHNET.COM
Free BioTechniques Past Paper Database
JEMERTON at BIOTECHNET.COM
Free BioTechniques Past Paper Database
JEMERTON at BIOTECHNET.COM
inducible drug resistance
JKHLab
inducible drug resistance
JKHLab
Biotrace membrane - Gelman
JLars36920
(none)
ITABAJARA DA SILVA VAZ JUNIOR
isotec
ITABAJARA DA SILVA VAZ JUNIOR
Capsaicin
Jack M. Bernstein, M.D.
luminous pen instead of radioactivity
Bill Jack
SSCP gels
Donna Jensen
RNA hyb solns?
JoeJoe
Thermocyler runs
JoeJoe
formaldehyde deact. w/ glycine?
JoeJoe
teflon tape
JoeJoe
XTT assay
Geir Johannessen
DNA from formaldehyde preserved specimens
JohnS52765
TaKaRa Single-Site Amplification
David Johnson
Capillary Electrophoresis
Todd Johnson
Capillary Electrophoresis equipment
Todd Johnson
DNA FROM HAIR
Todd Johnson
native gel
Todd Johnson
teflon tape
(Dave Johnston)
Hybaid products
(Dave Johnston)
Long gel dryers
(Dave Johnston)
Olomoucine, a cdc2 Protein Kinase inhibitor?
Mandy Johnstone
ss DNA expression in Mammalian cells
Dr. M.D. Jones
Question on generating nicked circular DNA using EthBr and EcoRI
Zophonias O. Jonsson
subscription
JOHN A. KORTE
hybridization bags
Ron Kagan
Epitopes for prokaryotic proteins
Kah-Tong, Seow
How do you measure rat's blood pressure???
Stefan Kahlert
Test,please ignore
Fredrik Kamme
Electron microscopy of Plasmid
Ali Karami
Denatured DNA and fixation
Frank Karlsen
Fixation of DNA close to protein
Frank Karlsen
south-western blot
Dr Simminder Kaur
pRc/CMV containing lacZ
Yoshiki Kawabe
PCR from fungal colonies
J Kennedy
ANTIBIOTIC STORAGE
Martin Kennedy
Biotrace membrane - Gelman
Martin Kennedy
gene targeting -- homologous recombination -- why not liposomes?
Martin Kennedy
Does no polyadenylation site mean no polyadenylation?
Martin Kennedy
Bulk prep problems
Martin Kennedy
non-rad colony screening
Roger Kennett
Why MOPS for RNA gels?
Oleh Kevalchuke
RNA elution from agarose
Oleh Kevalchuke
KLENTAQ manufacturer
In C. Kim
Designing Ideal PCR Primers
In C. Kim
Dimerization of plasmid in mini-preped sample?
Jinsuk Kim
Advice on Film Recorders
Mike Kitamura
News Posting..eg/
Klaus.Matthaei at ANU.EDU.AU
EXONUCLEASE III
Klaus.Matthaei at ANU.EDU.AU
Riboprobe background hybridisation
Klaus.Matthaei at ANU.EDU.AU
Long gel dryers
Klaus.Matthaei at ANU.EDU.AU
Riboprobe background vector
Klaus.Matthaei at ANU.EDU.AU
Maybe a stupid PCR question & a ETBr question
Klaus.Matthaei at ANU.EDU.AU
luminous pen instead of radioactivity
Dorothy Klein
BPDE-DNA
Hannes Klump
DISCUSSION - plasmid piracy = software piracy ?
Alex Knight
ECL for colony hybridization
Rinkei Ko
Cheaper electroporation cuvettes?
Eric Kofoid
How do you measure rat's blood pressure???
Steve Konings
what is the best miniprep
Sasha Kraev
Dye to colour DNA samples fro PCR
Sasha Kraev
SDS problem
Sasha Kraev
cycseq question/YAC sequencing
Sasha Kraev
Phage plaque PCR
Sasha Kraev
direct seq of PCR products
Sasha Kraev
Re. direct seq of PCR products/Epicentre Kits
Sasha Kraev
Long Ranger Gels
Sasha Kraev
Long Gel Dryers
Sasha Kraev
long run sequencing
Sasha Kraev
plasmid dimerization
Sasha Kraev
hot start in PCR(cycle seq)
Sasha Kraev
NaAc pptn
Jeff Kramer
nucleobond AX
Jeff Kramer
downward DNA transfer?
Cornelius Krasel
receptor cloning
Cornelius Krasel
In search of the "Holy manual"
Cornelius Krasel
Promega on the Internet
Cornelius Krasel
PacI weirdness
Cornelius Krasel
Sequencing vector Needed !
Cornelius Krasel
Codon Usage and Expression in E. coli
Cornelius Krasel
Baculoviral Info:
Cornelius Krasel
Extraction of DNA from agarose gels
Cornelius Krasel
synthetic proteins
Cornelius Krasel
Free BioTechniques Past Paper Database
Cornelius Krasel
ammonium sulfate precipitation of proteins
Cornelius Krasel
RNA structure graphics software
Cornelius Krasel
Adding membrane signal to cytoplasmic protein
Cornelius Krasel
curing bacteria of plasmids
Candace Krepel
Motifs for txn factors?
Dubear Kroening
DNA/phenol problem
Stuart Kuhstoss
PCR gene splicing: help!
Klas Kullander
Cheaper electroporation cuvettes?
Klas Kullander
pRc/CMV containing lacZ
Klas Kullander
NIH-3T3 drug test
Klas Kullander
address for Dynatech?
Klas Kullander
UV-crosslinking of DNA-protien complex
L.Y.Chau
measuring peptide concentration-exactly!
STRUCTURAL NEUROBIOLOGY LAB
Small RNA and Polysome isolation
LACYP at wsuvm1.csc.wsu.edu
downward DNA transfer?
LEES at SASK.USASK.CA
Marker for Plant?
LEES at SASK.USASK.CA
DNA*
LEES at SASK.USASK.CA
Kunkel mutagenesis- Help needed
LEMAILLET
TOMATO cDNA library
LEMAILLET
Cleavage with NTCB
Fabrice PARMENTIER LESAGE
non-radioactive DNA sequencing
LKM100F at ODUVM.BITNET
Rf: Aggregation of membrane proteins
LOGAND
membrane protein aggregation
LOGAND
Rf: In search of the "Holy manual"
LOGAND
Rf: Antibodies to KDEL or kXXkx peptides
LOGAND
anti-KDEL and anti-KxxKx antibodies
LOGAND
Macherey-Nagel Nucleobond for Seq.?
LOGAND
Rf: Strength of 4ml Spec. Cuvettes
LOGAND
Antibody against neomycin phosphotransfera
Dan Rawlins Lab
is there an index, restr. enz. sources???
Dana Macelis-Roberts Lab
microwave miniprep of plasmids
S.A. Benson Lab
Help:microwave miniprep of plasmids
S.A. Benson Lab
Mouse H-2D or K genomic clone
Carlisle Landel
Enzymes in KGB?
Carlisle Landel
retinol, cholecalciferol, vitamin B12
Stephen R. Lasky
Reticulocyte Lysate
Stephen R. Lasky
Free BioTechniques Past Paper Database
Carl Lawyer
DNA*
Carl Lawyer
Sequencing YACS
Martin Leach
Extraction of DNA from agarose gels
Martin Leach
pRc/CMV containing lacZ
Martin Leach
antibody to luciferase commercially available?
Martin Leach
antibody to luciferase commercially available?
Martin Leach
Transfection of neuronal cell lines???
Martin Leach
cDNA library from wheat and soybean
Martin Leach
Where to buy genomic libraries?
Martin Leach
Inverse PCR
Martin Leach
internet for the mol. biologist
Martin Leach
internet for the mol. biologist
Martin Leach
Contamination in Nested PCR
Martin Leach
Assymetric PCR references
Martin Leach
CAT assays
Martin Leach
DISCUSSION - plasmid piracy = software piracy ?
Martin Leach
TA Vector
Martin Leach
random priming
Martin Leach
Luciferase Problems?
Martin Leach
Need help with Eczema
Harold O. Leduc
Help me!! - prmoter inversion vector
Lee, Jae-Hyun
Free BioTechniques Past Paper Database
Lee, Jae-Hyun
Address or phone needed
Donna Leeper
microwave miniprep of plasmids
K. Legate
FILTRATION
K. Legate
FILTRATION (K.LEGATE)
K. Legate
HA epitope tag problems
K. Legate
Active Rb protein Ab
Brett Levay-Young
help for oligo hybridization
Limin Li
screening plasmid libraries
Yuanhao Li
beta-scanner
Walt Lilly
retroviral DNA preps
Lorena C. Lim
Companies on the net
Jonas Lindeberg
Kunkel mutagenesis- Help needed
Jonas Lindeberg
Qick nuclear extract preparation ?
Jonas Lindeberg
Long gel dryers
Jonas Lindeberg
XL-PCR ?
Jonas Lindeberg
Sal I cloning problems
Jonas Lindeberg
5'- 3'
Lauri Lintott
formal/agarose gels
Lauri Lintott
subcloning lamda library
Lauri Lintott
cloning-lambda-inserts
Lauri Lintott
GeneClean versus Quiagen
Joseph Lipsick
luminous pen instead of radioactivity
Joseph Lipsick
Protein FPLC (HPLC) Systems
Jim Litts
non-rad colony screening
LizH277522
oligoscreening ?
Joan Rigau Lloveras
Fluorometer
Muhammad Lodhi
Circular Dichroism and aggregates
Mark Jason Logan
Oligo dT for RT?
Elwyn Loh
DEPC treated water in PCR
John W. Longshore
PCR of GC-rich genes
John W. Longshore
HELP!!! Electroporation and PEG
Alfredo Lopez
More PEG and Electrophoration
Alfredo Lopez
GST fusion: Solutions and more questions
Ann Loraine
Cheaper electroporation cuvettes?
Heather H Lord
Quantitation of Westerns
Jeanne Loring
temperature-dependence of DNA-gyrase
Dmitry A. Los
What's in Megascript buffer ?
Patric Lundberg
EcR plasmid from Hogness at Stanford, CA
Patric Lundberg
Quick response from Hogness lab!
Patric Lundberg
urea/SDS, guanidine/SDS PAGE
Patric Lundberg
Promega on the Internet
Peter Lundberg
How good is the Eagle Eye II ? Alternatives ?
Alan Lyons
protease cleaving Ser-Ala
M.A.Schmidt
ANTIBIOTIC STORAGE
MAD, MAD, MAD SCIENTIST
wizard prep of DNA
MAD, MAD, MAD SCIENTIST
DNA/phenol problem
MAD, MAD, MAD SCIENTIST
Bioventures DNA isolation Kit
MAD, MAD, MAD SCIENTIST
Long gel dryers
MBDMITRY at CCVAX.SINICA.EDU.TW
10 mers
DAVID MITCHELL
EtBr-again
KARI KAMMIOVIRTA MMKAB
Were we can buy cheapest DNA primers?
KIMMO KOIVU MMKAT_OPISK
Were we can buy cheapest DNA primers?
KIMMO KOIVU MMKAT_OPISK
DEPC treated water in PCR
AARON J. MOE
Quickprep mRNA isolation from Pharmacia
MONSTER
Why dATP ?
MONSTER
Which Kit?
MONSTER
direct sequencing of PCR products
MONTESCLAROS,LUZ,MISS
homologous transfection system
MUCHA at edv1.boku.ac.at
What's in Megascript buffer ?
MUNGO at UVVM.UVIC.CA
Formiminoglutamate
MURLEY,LAURA LEA,MS
silver staining of DNA
NEAL MUSTO
storage of RNA
NEAL MUSTO
PCRE:PCR PRIMER DESIGN
NEAL MUSTO
PCR crash
Emma Macfarlane
exonuclease I
Walter Malangone
35 mm slides of gels - help
Malcolm Moos Jr., M.D., Ph.D.
5' RACE
Malcolm Moos Jr., M.D., Ph.D.
Sequencing insert from Lambda gt10 vector- HELP!
Malcolm Moos Jr., M.D., Ph.D.
Why MOPS for RNA gels?
Malcolm Moos Jr., M.D., Ph.D.
Buffer for plaque phage lysis --> PCR ?
Malcolm Moos Jr., M.D., Ph.D.
Operon (?) Inc. - need Address etc...
Mandalaparthy
need protocol for pcr of phage from plates.
Mandalaparthy
need protocol for pcr:gt10vector, frm plaques.
Mandalaparthy
tritium-labelled proteins: best detection method
Jonathan B. Marder
Nonrad. ATP synthase assays?
Ferenc Marincs
Nonrad. ATP synthase assays?
Ferenc Marincs
simple protocol for colony PCR
Ferenc Marincs
RNA from bacteria
Ferenc Marincs
direct sequencing of PCR products
Steve Marsh
GC-rich region
Richard Kenneth Bruce Martel
Long gel dryers
Richard Kenneth Bruce Martel
cloning-lambda libraries
Richard Kenneth Bruce Martel
cold SSCP
Daoust Martin
freeze-sqeeze
Daoust Martin
book
Daoust Martin
GC-rich region
Daoust Martin
Inverse PCR
Viraj Master
Idaho Technology Air Thermo-Cycler... Any good?
Viraj Master
Best method for blunting ds DNA?
David Matthews
Spectrophotometer Help
Andrew McArthur
non-radioactive southerns
C. McConville
QUESTIONS ON PCR CONDITIONS W/ DEGENERATE PRIMERS
Stephen M. McCrain
Help-plasmid recovery from dead plate!
Tara McCready
Taq slippage in PCR
John H McDonald
Looking for a plasmid
John McDougall
>>>Help about formal/agarose gel <<<
Steve McKinney
Best strategy from peptide sequences to cDNA clone?
Steve McKinney
Does no polyadenylation site mean no polyadenylation?
Steve McKinney
Contamination in Nested PCR
Patty McManus
Reverse northerns,cDNA not hot enough.
Robert McMaster
Dye to colour DNA samples for PCR
Ciaran Meghen
Optimisation of Hair Extraction Protocol
Ciaran Meghen
ANCIENT DNA
Ciaran Meghen
Inosine-containing oligos
Grace Mendoza
RNA isolation out of OCT embedded sections?
Harry Menegay
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction"
David J. Meyer
Buffering sat'd NaI sol'ns
David J. Meyer
Bind-silane removal
David J. Meyer
B.M.s 5 minute ligations?
David Micklem
Single stranded DNA restriction??????
Mike Powell, PhD
temperature-dependence of DNA-gyrase
Steve Minchin
Problem with Native PAGE
Ding Ming
Detection of "Invisible" Proteins???
Ding Ming
Lambda clone isoln - Lambda fix
Robert E Minto
RT-PCR question
Sean David Moore
PCR problems
Sean David Moore
stimulation of BT-20's & MCF-7 cells
Philip Joseph Moos
PCR gene splicing: help!
Kevin Morano
PCR crash
Kevin Morano
immunoprecipitation
Ralf Morgenstern
storing protein samples? ****
Shahram Mori
secondary screening after differential display
Shahram Mori
cDNA Challenge*************
Shahram Mori
RT-PCR question
Shahram Mori
cDNA Challenge*************
Shahram Mori
RT-PCR question
Shahram Mori
Single stranded DNA restriction??????
Shahram Mori
ANCHORED-PCR
Shahram Mori
Capsaicin
Mike Morris
Optimisation of Hair Extraction Protocol
Mike Morris
NuSieve Agarose
Mike Morris
Q: Human cDNA libraries?
Mike Morris
Test Message - Don't read!
Peter Mulderry
Smallest Cloning Vector and Selectable Marker ...
Scott B. Mulrooney
cDNA library screens via PCR
Sandra Munro
ECL for colony hybridization
Pete Muriana
suggestion: include email address in publications
Pete Muriana
curing bacteria of plasmids
Pete Muriana
Why dATP ?
Pete M. Muriana
PHAGE DNA KITS?
T. J. Murphy
Best method for blunting ds DNA?
Bernard Murray
Best method of blunting DNA: SUMMARY and THANKS
Bernard Murray
>>>Help about formal/agarose gel <<<
Bernard Murray
Detection of "Invisible" Proteins???
Bernard Murray
Long-Range PCR
Mwatson at uniwa.uwa.edu.au
Beteine and inclusion bodies
Rik Myers
Shotgun sequencing assembly
Peter Myler
PCgene program
Peter Myler
DNA*
Peter Myler
detection of 5-methyl cytosine (bisulfite method)
Onno Myriam
detection of 5-methyl cytosine (bisulfite method)
Onno Myriam
detection of 5-methyl cytosine (third question))
Onno Myriam
Beteine and inclusion bodies
Daniel Mytelka
ABRF network
NISSON at BIOTECHNET.COM
Luciferase as internal control
John J Naleway
Promega on the Internet: Biotechnet
John J Naleway
Companies on the Net
John J Naleway
HRP substrate that fluoresces
John J Naleway
Luminescent vs fluorescent ELISA
John J Naleway
Marker for Plant?
John J Naleway
staining for beta-galactosidase
John J Naleway
Kits for Subtraction Libraries?
John Nash
Which PCR machine?
John Nelson
Capillary Electrophoresis
Robert Nelson
Need address for Molecular Probes Inc.
Robert Nelson
DNA Amplification Market
Robert Nelson
RNA Isolation
Robert Nelson
Were we can buy cheapest DNA primers?
Robert Nelson
PCR crash
Robert Nelson
Companies on the net
Rick Neubig
looking for calpain antibody
Robert Neumar
RT-PCR question
Gerri Newfry
PCgene program
Gerri Newfry
minipreps for sequencing
Thomas Newman
virus receptor research--Help
I-shiou Ng
Pharmacia GeneQuant DNA calculator opinions wanted
Prenom Nom
In search of the "Holy manual"
Frank Norman
Promega on the Internet: Biotechnet
Frank Norman
cDNA Challenge*************
Andy Law Big Nose
subscripte bionet.molbio.methds-reagnts
Danielle Nouaud
PCR journal
Kevin O'Donnell
Diagnostics Prototype Newsgroup
Kevin O'Donnell
Adding membrane signal to cytoplasmic protein
ORSULIC at UNCVX1.OIT.UNC.EDU
dig labelled Riboprobe whole mount insitu?
Dan Oerther
Ambion RPA II Kit??
Guilherme Co. Oliveira
kinases
Torben Osterlund
Detection of "Invisible" Proteins???
Torben Osterlund
sequence of pSP64(polyA) - Promega
Francis Ouellette
internet for the mol. biologist
Francis Ouellette
internet for the mol. biologist
Francis Ouellette
internet for the mol. biologist
Francis Ouellette
>>>>URGENT : IN VITRO TRANSCRIPTION <<<<<
Jim Owens
Long gel dryers
Jim Owens
DNA/phenol problem
Jim Owens
chemistry of Qiaex beads & DNA interaction??
PRAMATHESH PATEL
clean GST antibodies source?????
PRAMATHESH PATEL
Quickprep mRNA isolation from Pharmacia
PEDP043 at uabdpo.dpo.uab.edu
? Supplier of mRNA Diff. display kit
RICHARD P. PHIPPS
HA epitope tag problems
PJOB
How good is the Eagle Eye II ? Alternatives ?
PMacklon
Far-Western help
POLAKBM at WPOGATE.SLU.EDU
Prokaryotic fusion protein systems
POLAKBM at WPOGATE.SLU.EDU
Viral Cell Packaging line GP+E86
POLAKBM at WPOGATE.SLU.EDU
what's the best way to clone PCR product
POPOVA
single-copy vectors
POPOVA
Oligo dT for RT?
POPOVA
Coupling IgG via sugar residues
POPOVA
Problems w/ native PAGE
POPOVA
Method for Touchdown PCR
Paiks
Ghosts and membrane fluidity
Jan Palaty
ECL direct
Dieter Palmer
ECL direct
Dieter Palmer
XTT assay
Dieter Palmer
simple protocol for colony PCR
Anthony Palombella
hybridization bags
Dave Pataky
DIG-labelled antisense for RNAse protection?
Jose Nieto Path
Coupling IgG via sugar residues
Dave Pearton
Spin columns for DNA band excision?
Mario de la Pena
Need reliable method for RNA extraction from lymphocytes
Howard T. Petrie
Need reliable method for RNA extraction from lymphocytes
Howard T. Petrie
Spin columns for DNA band excision?
Howard T. Petrie
Requesting pGEM-luc and pSL301
daren Grossman - Pharmacology
hybridization bags
Genetic Phlegm
receptor cloning
T. S. Pillay
clean GST antibodies source?????
T. S. Pillay
anti-phosphoserine/threonine Ab?
T. S. Pillay
anti-phosphoserine/threonine Ab?
T. S. Pillay
? Supplier of mRNA Diff. display kit
T. S. Pillay
Tyrosine kinases in bacteria
T. S. Pillay
GST
T. S. Pillay
Immobilon CD membranes- do they work?
T. S. Pillay
Extraction of DNA from Agarose Gel
T. S. Pillay
beta-globin antibodies?
Geoff Pinchbeck
How annoying (was Re: RT-PCR question)
Michael Poidinger
Cutting Edge (Was Re: Recent DNA and RNA isolation methods wanted)
Michael Poidinger
posting to bionet
Michael Poidinger
Transfection of neuronal cell lines???
Michael Poidinger
staining for beta-galactosidase
Michael Poidinger
Sequencing with hexamers (was degenerate primers)
Richard T. Pon
Companies technical information supply online
Bert Popping
Yeast cDNA library kit
Bert Popping
Storage of RNA
Rosamund Eira Powles
Electroelution and deglycosylation
Prasad, Krishna
is there an index, restr. enz. sources???
Robert Preston
Thermocyler runs
Robert Preston
Fax of Genelabs Technologies
Otto Pritsch
LacZ in eukaryot. promoter studies
Przemko
Question on generating nicked circular DNA using EthBr and EcoRI
Rock Pulak
Kits for Subtraction Libraries?
Kevin Pumiglia
Adding membrane signal to cytoplasmic protein
Kevin Pumiglia
Pharmacia GeneQuant DNA calculator opinions wanted
PENNY RIGGS
anti-Taq polymerase antibodies for hotstart
RMARTINQFD at BIOTECHNET.COM
Need Advice on Film Recorders
RNeubig at umich.edu
hemagglutinin epitope for Westerns
ROSS at SCI.WFEB.EDU
Aggregation of membrane proteins
Dayanidhi Raman
Info. on refrigerated microfuge
Ramanujam Raman
Stability of Heparin
Ramanujam Raman
rat cDNA library
Mukul Ranjan
HRP substrate that fluoresces
Rascals
Extraction of DNA from Agarose Gel
Laurence T. Reiter
Transient transfection assays.
Laurence T. Reiter
PFGE and YACs
Department of Research
direct sequencing of PCR products
Department of Research
Sequencing with degenerate primers
RichWalko
35 mm slides of gels - help
Richard
Promega on the Internet
Richard
Tritium screen 0 : EnHance 1
Richard
Tritium screens
Richard
ss DNA expression in Mammalian cells
Richard
Artifacts in silver staining
Richard
Homebrew saline for Contact Lenses
Brad Riesenberger
Membrane protein fusion vectors? Know of any?
Paul Riggs
sequence of pSP64(polyA) - Promega
Robert.Coelen at jcu.edu.au
simple protocol for colony PCR
Robert.Coelen at jcu.edu.au
RTPCR/degenerate primers with inosine?
Robert.Coelen at jcu.edu.au
Summary: simple protocol for colony PCR
Robert.Coelen at jcu.edu.au
EXONUCLEASE III
Michael Austin Roberts
Sequencing YACS
Bruce Roe
Cycle Sequencing Question
Bruce Roe
help for oligo hybridization
Gabriel Romero
Baculoviral Info:
Brad Romney
PFGE and YACs
Elizabeth Rosenberg
S1 Nuclease kits
Traci Roth
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction"
Traci Roth
CAT assays
Traci Roth
Homebrew saline for Contact Lenses
Robert Rumpf
In search of the "Holy manual"
SPAGNOL at PLAUTO.CSATA.IT
Phosphoimagers vs Instantimager
Roland J Saldanha
Phosphoimagers vs Instantimager
Roland J Saldanha
E. coli lacZ complementation
Roland J Saldanha
oligonucleotide synthesizers
Roland J Saldanha
what's the best way to clone PCR product
Klaus Salger
small DNA fragment recovery
Klaus Salger
non-isotopic gel shift assays
Klaus Salger
Best method for blunting ds DNA?
Klaus Salger
Glowing BLUE DNA Gels
Klaus Salger
is there an index, restr. enz. sources???
Klaus Salger
Codon preferences of differrent organisms
Klaus Salger
----URGENT : RNAase Free Water ?----
Klaus Salger
teflon tape
Klaus Salger
DIG system for Northern blotting
Klaus Salger
Long gel dryers
Klaus Salger
EtBr-again
Klaus Salger
Enzymes in KGB?
Klaus Salger
request advice on mRNA preparation
Klaus Salger
Wanted - buffer systems for pH range 4 to 9 in cell culture expt.
Mr. P.A. Sansom
homocystamine (aminopropanethiol)
Santhosh
RT-PCR question
Adi Santoso
pIN-III-ompA-hind
Dr. Jon R Sayers
Plasmid collections-where?
Dr. Jon R Sayers
luminous pen instead of radioactivity
Leo Schalkwyk
Codon Usage and Expression in E. coli
Rebecca Schall
Bioventures DNA isolation Kit
Rebecca Schall
Transfection of oesophageal (squamous cell)carcinoma cell line
Irene Schauer
DNA-affinity columns
Alan Schoenfeld
dig labelled Riboprobe whole mount insitu?
P. Jones/ P. Scotting
Wanted Japan/uk fellowship candidate
P. Jones/ P. Scotting
PC-based plasmid map programs
Hank Seifert
PCgene program
Hank Seifert
ATCC site
Loryn Sellner
Homebrew saline for Contact Lenses
Loryn Sellner
Homebrew saline for Contact Lenses
Loryn Sellner
RNA from paraffin embedded tissue
Loryn Sellner
ANNOUNCING: ALF DNA Sequencer Discussions in bionet.genome.chromosomes
Jackson Shea
Sequencing problem, HELP!!
Glen Shearer
RNA hyb solns?
Glen Shearer
Why MOPS for RNA gels?
Glen Shearer
PHAGE DNA KITS?
Glen Shearer
long ranger gels
Glen Shearer
Ziplox - expressible?
J Shepherd
electroporation
Yoshiyuki Shibata
Maybe a stupid PCR ques
Margaret Shirra
GeneClean versus Quiagen
Michael Sidler
half life of GUS, luciferase and other reporters?
Jeffrey Alan Silverman
MEK phosphorylation
Jeffrey Alan Silverman
storing protein samples? ****
Robert Slany
Promega on the Internet
Dave Smith
Sequencing with degenerate primers
Dave Smith
pRc/CMV containing lacZ
Dave Smith
>>>Help about formal/agarose gel <<<
S L Smithson
anti-phosphoserine/threonine Ab?
Jae-Won Soh
keratinase / alkaline protease
Tony Soltis
HA epitope tag problems
Anne Spang
Western Using Monoclonals
Anne Spang
test
Charlie Spillane
Which PCR machine?
Darren Stauth
Maybe a stupid PCR question & a ETBr question
Darren Stauth
DNA/phenol problem
Darren Stauth
Anyone using "hot start" cycle sequencing?
Darren Stauth
cDNA kits?
Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
ANTIBIOTIC STORAGE
Stephen_W._Mamber_at_~PRIWFB05 at ccmail.bms.com
What's the best mini-prep?
B Stickney
Detection of rRNA methylation
B Stickney
Tris and DEPC
B Stickney
Info. on refrigerated microfuge
B Stickney
Storage of RNA
B Stickney
PostDoc Molecular Virology New Jersey (job)
Vic Stollar
Kits for Subtraction Libraries?
John Storring
Volatile buffers info (TEAB; TEAA)
Gregory Stuart
promoter search
Satyam Subrahmanyam
Geiger Counter
Satyam Subrahmanyam
(none)
Zalman L. Suldan
electroporation cuvettes
Zalman L. Suldan
Extraction of DNA from agarose gels
Clemens Suter-Crazzolara
receptor cloning
Clemens Suter-Crazzolara
protein precipitates:HELP !
Clemens Suter-Crazzolara
protein precipitates:HELP !
Clemens Suter-Crazzolara
SDS Problem
Clemens Suter-Crazzolara
protein precipitates:HELP !
Clemens Suter-Crazzolara
teflon tape
Clemens Suter-Crazzolara
Does no polyadenylation site mean no polyadenylation?
Clemens Suter-Crazzolara
PCR crash
Clemens Suter-Crazzolara
Ab for 6XHis tag??
Clemens Suter-Crazzolara
Smallest Cloning Vector and Selectable Marker ...
Clemens Suter-Crazzolara
Rotofor preparativ IEF
Jan Svensson
What is the perfect PCR?
Agnieszka Szczepek
yeast genomic library
PAUL TASLIMI
Yeast genomic Library
PAUL TASLIMI
Why MOPS for RNA gels?
NICHOLAS THEODORAKIS
VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY
NICHOLAS THEODORAKIS
>>>Help about formal/agarose gel <<<
NICHOLAS THEODORAKIS
Detection of "Invisible" Proteins???
NICHOLAS THEODORAKIS
Dynatech address
Tahilyamani at pahkr6.Syntex.Com
urea/SDS, guanidine/SDS PAGE
Song Tan
Sal I cloning problems
Lisa Taylor
Syringe pump WANTED
Lorne Taylor
Anti-LexA protein antibodies??
Dennis Templeton
long ranger gels
Peter Thorén
Idaho Technology Air Thermo-Cycler... Any good?
Peter Thorén
Clontech Ligation Express
Richard T. Timmer
Westerns to detect HA Tag?
ToddS41
Promega on the Internet
Anthony Tomlinson
q
Bianca L Torrez
ANCIENT DNA
Chris Troy
ANCIENT DNA
Chris Troy
Corvas International
David Tuminaro
(none)
Bruce J. Turner
oligo hybridization
Bruce J. Turner
GST fusion: Solution and more questions
Rodney Tweten
T4 ENDONUCLEASE VII
URA1335 at ARTHUR.CITI2.FR
T4 ENDONUCLEASE VII ???
URA1335 at ARTHUR.CITI2.FR
hybridization bags
Udo Schlosser, D1.4.03, Tel.: 6253
GAL4 fusion/expression vectors ?
Peter Uetz
Sequencing with degenerate primers
Chris Upton
Home made glass FOG/MIST request
Chris Upton
DISCUSSION - plasmid piracy = software piracy ?
Chris Upton
SS amplification woes
VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
enhancer dependent color
Varyc
HIV-1 panel
Varyc
Shotgun sequencing assembly
Varyc
Alk phos conjugation
Varyc
Detection of "Invisible" Proteins??? - Reply
Varyc
error free proteins
Varyc
mycobact databases
Varyc
minipreps for sequencing
Varyc
Geiger Counter
Paul A. Vescio
teflon tape
Paul A. Vescio
problem with native PAGE
Nora Vig
Help needed with alkaline phosphatase conjugation to oligonucleotides
Dominic Voon
direct sequencing of PCR products
Karl Voss
Need reliable method for RNA extraction from lymphocytes
Ben Vowels
minipreps for sequencing
Stephane Vuilleumier
promega wizard kits and the 373
WAS
Promega on the Internet
WICKHAM,JOHN
Expression problems
WICKHAM,JOHN
E. coli lacZ complementation
HERBERT WYCKOFF
Pseudomonas/bacteriocins
FJ van der Wal
Hair Follicle DNA Fingerprinting
Shiao Y. Wang
Dye to colour DNA samples for PCR
Shiao Y. Wang
Taq slippage in PCR
Shiao Y. Wang
Help!!! Removing polysaccharides from DNA preps
Devaka Weerakoon
HELP: NORTHWESTERN ASSAY
John J Weiland
Oligonucleotides as Linkers?
Weiss
Promega on the Internet
Helge Weissig
Promega on the Internet
Patrick Weix
Best method for blunting ds DNA?
John J Welch
DNA/phenol problem
Jennifer_ Welsh
ask for information
Wenfu
anti-Taq polymerase antibodies for hotstart
Carolyn M Wetzel
Macherey-Nagel Nucleobond for Seq.?
Frank White
antibody to luciferase commercially available?
Sheryl White
antibody to luciferase commercially available?
Sheryl White
rBGH organism
Roger Wiegand
error free proteins
Roger Wiegand
Extraction of DNA from agarose gels
Wiggy
DNA purification
Wiggy
help: protocol for removal of dye terminators for cyc. seq.
John Williford
Kunkel mutagenesis- Help needed
Berthe M. Willumsen
Tritium screens / protein gels
Berthe M. Willumsen
TRANSFAC database
Edgar Wingender
Seeking ELISA plate reader advice
Donald Chun Kit Wong
Seeking ELISA plate reader advice
Donald Chun Kit Wong
ELISA microplate reader
Donald Chun Kit Wong
GST
Surasakdi Wongratanacheewin
kinases
Jim Woodgett
anti-phosphoserine/threonine Ab?
Jim Woodgett
Tyrosine kinases in bacteria
Jim Woodgett
HA epitope tag problems
Jim Woodgett
Pharmacia GeneQuant DNA calculator opinions wanted
Robert Woodward
luminous pen instead of radioactivity
Robert Woodward
Human cell transformation- SOD1 and SOD2 genes
Brad Wouters
inverse PCR & the price of anchovies
B.W. Wren
inverse PCR & the price of anchovies
B.W. Wren
sig
John Wurzelmann
protein expression - vessel size: summary
XRDPROC at Gems.VCU.EDU
silica based DNA extraction
Jinghai Xu
dhfr antibody
Min Xu
Help! DNA insoluble in water
Nanfel Xu
Problem with Native PAGE
Pin Xu
Best method for blunting ds DNA?
Annie Yang
qiagen column regeneration
Annie Yang
Phosphoimagers vs Instantimager
Ian A. York
35 mm slides of gels - help
Ian A. York
GeneClean versus Quiagen
Ian A. York
Help! DNA insoluble in water
Ian A. York
Co-transfection methods
Ian A. York
SDS Problem
Lester Young
tandem signal sequences
Pak-Lam Yu
tandem signal sequences
Pak-Lam Yu
Need address for Molecular Probes Inc.
Paquette Yves
Sequencing problem, HELP!!
Zainul Zain
Methionine-free insect cell culture media
Matthias Zeiner
clean GST antibodies source?????
Matthias Zeiner
mutiple and faint bands in PCR
Xin-Hua Zhou
UV-crosslinking of DNA-protien complex
Frances Hannan Zoology
Info. on refrigerated microfuge
Frances Hannan Zoology
Direct sequencing for ABI 373
a.cox at rbgkew.org.uk
VectorNT1 experiences?
adler at staff.monash.edu.au
FILTRATION
ae55
FILTRATION (K.LEGATE)
ae55
How do you transform aorta-smooth muscle cells?
aeschba at fmi.ch
Substitute for F12 K-media for HUVECs wanted
aeschba at fmi.ch
Info on Dynal magnetic beads (efficiency of extraction)
ajayaram at pearl.tufts.edu
oligonucleotide synthesizers
altabios.internet{altabios at bham.ac.uk}
Taq pol patent update
an125249 at anon.penet.fi
Taq pol patent update
an125249 at anon.penet.fi
UV-crosslinking of DNA-protien complex
anon
long film & cassettes for sequencing - where?
anon
NaAc precipitation
anon
Help! DNA insoluble in water
anon
Extraction of DNA from Agarose Gel
anon
help
ahmet arman
PCgene program
b137795 at mailserv.cuhk.hk
RoboCycler?
dudy bar-zvi
Plasmid copy number control
bchs1b
Dimerization of plasmid in mini-preped sample?
benedik at uh.edu
Codon preferences of differrent organisms
bio4 at ccnet.up.ac.za
random priming
braam at BIOC.RICE.EDU
PacI weirdness
brett at BORCIM.WUSTL.EDU
small DNA fragment recovery
lesley brown
lexA-antibody
mingzi chen
RACE, need help
chien at mbcf.stjude.org
QUICKHYBE from Stratagene - opinions?
steven joseph chmura
MATCHMAKER Two-Hybrid Supplement
clontech!pauld at NETCOM.COM
refrigerated cent.
colvin at ouvaxa.cats.ohiou.edu
DNA sequencing services
cote at bscr.uga.edu
Measuring Plant Cell Death
cote at bscr.uga.edu
Spin columns for DNA band excision?
crawford at bscr.uga.edu
RT storage?
croyleml at ucbeh.san.uc.edu
What's the best condition for transfection of most commonly used cell lines?
Aurel d'etre
Desperation - how do I get off?
dacoj3 at wptemp.kvl.dk
How good is the Eagle Eye II ? Alternatives ?
dallas at mcvax4.d48.lilly.com
ammonium sulfate precipitation of proteins
bipin dalmia
Quantitative protein determinations in plant extracts
bipin dalmia
Some Receptor Theory Questions...Constitutive or not?
davis at wehi.edu.au
pulse field gels
shelly may deane
Phosphoimagers vs Instantimager
dhanzel at delphi.com
Quantitation of Westerns
dhanzel at delphi.com
non-radioactive southerns
dhanzel at delphi.com
Luminescent vs fluorescent ELISA
dhanzel at delphi.com
Need address for Molecular Probes Inc.
dhanzel at delphi.com
luminous pen instead of radioactivity
dkatz at doane.edu
Help need for Single-step RNA Ioslation by Acid Guanidinium
Thiocyanate-Phenol-Chloroform Extraction"
dkim at unm.edu
FILTRATION (Gelman Sciences)
donnas at gelman.com
PCR of GC-rich genes
doug_mckenzie at stratagene.com
ECL for colony hybridization
dpetersn at unixg.ubc.ca
direct seq pcr w/abi
eckloff at rcf.mayo.edu
Hot phenol prep, genomic DNA
enof
Hot phenol prep, genomic DNA
enof
PCR from fungal colonies
enof
ammonium sulfate precipitation of proteins
larson eric
Sequencing vector Needed !
f.r.byrne
Cancer Treatment Groups
feco1104 at altair.selu.edu
cDNA Challenge*************
douglas l feinstein
RT-PCR question
douglas l feinstein
Buffer for plaque phage lysis --> PCR ?
fgrun
PC-based plasmid map programs
nicola fidler
Glowing BLUE DNA Gels
fnord
Homemade GeneClean kits ???
fnord
Vistra
frits at hubrecht.nl
Extraction of DNA from agarose gels
futers at biovax.leeds.ac.uk
DNA mis-match analysis
futers at biovax.leeds.ac.uk
pcDNA3 with Wizard
g_watson at icrf.icnet.uk
extraction of DNA from agarose gels
g_watson at icrf.icnet.uk
pSV2dhfr seqeunce anyone?
g_watson at icrf.icnet.uk
pRc/CMV containing LacZ
g_watson at icrf.icnet.uk
ss DNA expression in Mammalian cells
g_wenck at spike.psc.scarolina.edu
teflon tape
gc
urea/SDS, guanidine/SDS PAGE
gflentke.internet{gflentke at facstaff.wisc.edu}
urea/SDS, guanidine/SDS PAGE
gflentke at facstaff.wisc.edu
Detection of "Invisible" Proteins???
ggordon at opal.tufts.edu
wizard prep of DNA
gibbs at sbsu1.auckland.ac.nz
temperature-dependence of DNA-gyrase
christophe gilbert
Sequencing problem, HELP!!
gli at UCDAVIS.EDU
SSCP analysis protocol
gli at UCDAVIS.EDU
cold SSCP
gli at UCDAVIS.EDU
subscribe
gr8bn at cc.usu.edu
HELP: NORTHWESTERN ASSAY
gr8bn at cc.usu.edu
long ranger gels
gustilo at pobox.upenn.edu
Anyone using "hot start" cycle sequencing?
gustilo at pobox.upenn.edu
Need Advice on Film Recorders
gustilo at pobox.upenn.edu
Plasmid copy number
jim hartley
Plasmid copy number control
jim hartley
CAT assays
henderson
oligonucleotide synthesizers
hl15 at hubrecht.nl
half life of GUS, luciferase and other reporters?
hraaxl at marc.cri.nz
southern and northern on the same blot
hrichter at ulys.unil.ch
GC-rich region
hschweiz
use of sodium salicylate prior to autoradiography
hschweiz
Canadian Novagen Users
intermed at inforamp.net
Amplification of pACYC184 plasmid
jalexand at crihan.fr
txn factor consensus seq. database??
jboatri.internet{jboatri at eagle.cc.emory.edu}
KLENTAQ manufacturer
jchurch at kean.ucs.mun.ca
Which PCR machine?
jfh
TIGR or HGS address?
jfh
test JPF
jfield
Long range Genomic PCR
jlanders
Bioscience Archives?
jnewton at molbiol.ox.ac.uk
TA Vector
jnewton at molbiol.ox.ac.uk
KLENTAQ manufacturer
kamin johnson
silica based DNA extraction
jxu.internet{jxu at MIT.EDU}
separating large DNA fragments-info needed
kannan at kuhub.cc.ukans.edu
Radiographic Duplicating Unit
kramerse at wmvx.lvs.dupont.com
oligos
kreuzer at bkfug.kfunigraz.ac.at
Smallest Cloning Vector and Selectable Marker ...
Allin Verreault in Stillman lab
Extraction of DNA from agarose gels
leshof at delphi.com
Seeking ELISA plate reader advice
littgj at wmvx.lvs.dupont.com
HRP substrate that fluoresces
littgj at wmvx.lvs.dupont.com
Luminescent vs fluorescent ELISA
littgj at wmvx.lvs.dupont.com
qiagen column regeneration
elias lolis
Returned mail: User unknown
lw75
synthetic proteins
lw75
small DNA fragment recovery
m-brazil at nimr.mrc.ac.uk
Phosphoimagers vs Instantimager
macIIci
Minipreps for ABI sequencer
???? maria
SDS Problem
john markwell
Baculoviral Info:
stephen marshalko
Ecoli periplasmic secretion vectors?
mbsay at seqnet.dl.ac.uk
How good is the Eagle Eye II ? Alternatives ?
mcgeed at HAL.HAHNEMANN.EDU
Electroporation cuvettes
mcgeed at HAL.HAHNEMANN.EDU
Electroporation cuvettes
mcgeed at HAL.HAHNEMANN.EDU
Electroporation cuvettes
mcgeed at HAL.HAHNEMANN.EDU
Capsaicin
mdaily at delphi.com
Maybe a stupid PCR question & a ETBr question
mdcabl at cc.newcastle.edu.au
baculovirus and receptors
megVx
antibody database on the Net
megVx
Announcement: 2 Hybrid System Review Available on MGH Gopher Server
mendelsohn at Frodo.MGH.Harvard.EDU
Genesis Pharmaceuticals (Cambridge, MA) -- Combinatorial Chemistry ???
merlin
suggestion: include email address in publications
merlin
gene targeting -- homologous recombination -- why not liposomes?
merlin
Capsaicin
mhughes at mbcf.stjude.org
Long gel dryers
mhughes at mbcf.stjude.org
Adding membrane signal to cytoplasmic protein
mhughes at mbcf.stjude.org
E. coli lacZ complementation
mike
MSE Centrifuges (Help!)
mlrjsg at dct.ac.uk
PCR problems
mwatson at uniwa.uwa.edu.au
Promega on the Internet
robert nusbickel
chaps
nussbaum at neurochem.u-strasbg.fr
HELP: NEEDS A PIG rDNA CLONE
opp at icbr
SSCP analysis protocol
orbashu at ubvms.cc.buffalo.edu
Idaho Technology RAPD analysis
palasj9579 at cobra.uni.edu
cold SSCP
parkef at delphi.com
Need address for Molecular Probes Inc.
patel_p at bms.com
hybridization bags
patterson at frodo.mgh.harvard.edu
in situ PCR
pattonaj%phvax.dnet at sb.com
nuclear proteins
pattonaj%phvax.dnet at sb.com
kit for differential display
pattonaj%phvax.dnet at sb.com
westerns using monoclonals
pattonaj%phvax.dnet at sb.com
PCR gene splicing: help!
pskaytes at INTNET.UPJ.COM
Epitopes for prokaryotic proteins
wang qi
Western Using Monoclonals
wang qi
check mail.
ranga at vigyan.iisc.ernet.in
hybridization bags
doug rhoads
RT-PCR question
rhubner at molbiol.ox.ac.uk
Storage of RNA
rhubner at molbiol.ox.ac.uk
oligo(dT) with single base at 3'-end...
rhubner at molbiol.ox.ac.uk
Single stranded DNA restriction??????
rnwil003 at uctvax.uct.ac.za
Single lane/column sequencing-help
rnwil003 at uctvax.uct.ac.za
Promega on the Internet
robert at chironex.jcu.edu.au
Sal I cloning problems
rpgrant at molbiol.ox.ac.uk
Does no polyadenylation site mean no polyadenylation?
rr at BMMB01.UMESCI.MAINE.EDU
PCR of GC-rich genes
sbaker at molbiol.ox.ac.uk
Summary: PCR of GC-rich genes
sbaker at molbiol.ox.ac.uk
incorporating biotin-dNTPs
schoenfeld at MSSCC.MED.UTAH.EDU
PCR PRIMER DESIGN
schupp at nauvax.ucc.nau.edu
posting to bionet
schupp at nauvax.ucc.nau.edu
DNA purification
schupp at nauvax.ucc.nau.edu
EXONUCLEASE III
schupp at nauvax.ucc.nau.edu
DISCUSSION - plasmid piracy = software piracy ?
schupp at nauvax.ucc.nau.edu
Amplification of pACYC184 plasmid
schupp at nauvax.ucc.nau.edu
Keystone Oncogenes mtg: Want Roommate
seklund.internet{seklund.internet{seklund at netserv.unmc.edu}
Keystone Oncogenes mtg: Want Roommate
seklund.internet{seklund at netserv.unmc.edu}
Luciferase Problems?
sfraser.internet{sfraser at acs.ucalgary.ca}
pcDNA3 with Wizard
lee shekter
pCI
lee shekter
QUICKHYBE from Stratagene - opinions?
sjchmura.internet{sjchmura at kimbark.uchicago.edu}
ANCHORED-PCR
sl6dp at cc.usu.edu
non-isotopic gel shift assays
slee at opal.tufts.edu
Need dephosphorylation protocol
slee at opal.tufts.edu
help about formal/agarose gels
slee at opal.tufts.edu
Need RNA structure program
slee at opal.tufts.edu
Cheaper electroporation cuvettes?
szcooley at dale.ucdavis.edu
electroporation cuvettes
szcooley at dale.ucdavis.edu
KLENTAQ manufacturer
tebas_p at msdisk.wustl.edu
gene-capsule
thomas
Alsever's Solution
henderson todd
isolation of nuclei from lymphocytes
michelle tourigny
test
troianovsk_s at msdisk.wustl.edu
Codon Usage and Expression in E.coli.
vaughan at sci.wfeb.edu
Inverse PCR?
vdrover at kean.ucs.mun.ca
Companies on the Net
walling at wmvx.lvs.dupont.com
address of So. Cal forensic lab
walterh.internet{walterh at u.washington.edu}
Topology of a multisubunit membrane protein
wegloehner at mpimg-berlin-dahlem.mpg.de
Luciferase reporter gene with beta actin promoter
x91holmen at wmich.edu
Luciferase reporter gene/beta actin promoter
x91holmen at wmich.edu
problem with pUC18..help me !!
Lee jang young
Overexpression with pKK223-3 !! help
Lee jang young
Best strategy from peptide sequences to cDNA clone?
yqhuang at iastate.edu
Electroelution and deglycosylation
yqhuang at iastate.edu
PCR PRIMER DESIGN
yu at ouvaxa.cats.ohiou.edu
Last message date:
Mon Oct 31 23:05:18 EST 1994
Archived on: Sun Mar 6 21:26:25 EST 2005
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