Methods October 1994 Archives by date

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Starting: Sat Oct 1 07:08:14 EST 1994
Ending: Mon Oct 31 23:05:18 EST 1994
Messages: 1221

small DNA fragment recovery m-brazil at nimr.mrc.ac.uk
what's the best way to clone PCR product POPOVA
DNA sequencer Eric C. Anderson
promoter search Satyam Subrahmanyam
35 mm slides of gels - help Richard
Promega on the Internet Richard
yeast genomic library PAUL TASLIMI
Yeast genomic Library PAUL TASLIMI
cDNA Challenge************* douglas l feinstein
Taq left at RT - gulp! - Susan Cole
Tritium screen 0 : EnHance 1 Richard
Are Ab's phosphorylated? Michele Fuortes
Hair Follicle DNA Fingerprinting Shiao Y. Wang
Sequencing problem, HELP!! Zainul Zain
tritium-labelled proteins: best detection method Jonathan B. Marder
single-copy vectors POPOVA
virus receptor research--Help I-shiou Ng
RT-PCR question Adi Santoso
what's the best way to clone PCR product Klaus Salger
small DNA fragment recovery Klaus Salger
Far-Western help POLAKBM at WPOGATE.SLU.EDU
Prokaryotic fusion protein systems POLAKBM at WPOGATE.SLU.EDU
separating large DNA fragments-info needed kannan at kuhub.cc.ukans.edu
BPDE-DNA Hannes Klump
Capsaicin mdaily at delphi.com
Phosphoimagers vs Instantimager Graham Atherton
ABI sequencing and Stratagene Taq Extender Duncan Clark
POTATO SOMATIC MUTATIONS Dickinson, Harry
S1 nuclease/ exo III Fabrice Glibert
Promega on the Internet Dave Smith
Dye to colour DNA samples for PCR Ciaran Meghen
Extraction of DNA from agarose gels Yasha Hartberg
small DNA fragment recovery lesley brown
How do you transform aorta-smooth muscle cells? aeschba at fmi.ch
Extraction of DNA from agarose gels futers at biovax.leeds.ac.uk
Phosphoimagers vs Instantimager Peter Heifetz
Promega on the Internet Anthony Tomlinson
35 mm slides of gels - help Andrew Cockburn
William D. Huse / Ixsys, Inc -- San Diego, CA H. David Fischer
kinases Torben Osterlund
cDNA Challenge************* Andy Law Big Nose
pcDNA3 with Wizard lee shekter
Fax of Genelabs Technologies Otto Pritsch
pcDNA3 with Wizard g_watson at icrf.icnet.uk
Minipreps for ABI sequencer ???? maria
Glowing BLUE DNA Gels Paul N Hengen
extraction of DNA from agarose gels g_watson at icrf.icnet.uk
promega wizard kits and the 373 Eric C. Anderson
cDNA Challenge************* Annette C. Hollmann
kinases Jim Woodgett
IgM Shaun D. Black
non-isotopic gel shift assays slee at opal.tufts.edu
Need dephosphorylation protocol slee at opal.tufts.edu
Dye to colour DNA samples for PCR Robert Horton
Some Receptor Theory Questions...Constitutive or not? Dave
non-isotopic gel shift assays Klaus Salger
Hair Follicle DNA Fingerprinting Karen Browning
Mouse H-2D or K genomic clone Carlisle Landel
downward DNA transfer? LEES at SASK.USASK.CA
Rotofor preparativ IEF Jan Svensson
Taq slippage in PCR John H McDonald
pcDNA3 with Wizard Yasha Hartberg
rat cDNA library Mukul Ranjan
SDS Problem Lester Young
Capsaicin Jack M. Bernstein, M.D.
gene-capsule thomas
storing protein samples? **** Shahram Mori
secondary screening after differential display Shahram Mori
cDNA Challenge************* Shahram Mori
Problem with Native PAGE Pin Xu
CAPILLARY ELECTROPHORESIS equipment ? Timo Hiltunen
WANTED: Old liquid scintillation counters Ron Berry
Best method for blunting ds DNA? Bernard Murray
Extraction of DNA from agarose gels Clemens Suter-Crazzolara
Phosphoimagers vs Instantimager Nigel Eastmond
Storage of RNA Rosamund Eira Powles
receptor cloning Clemens Suter-Crazzolara
DNA mis-match analysis futers at biovax.leeds.ac.uk
What's the best mini-prep? C.J. Hussussian, MD
Test George F Edick
Phosphoimagers vs Instantimager Ian A. York
Promega on the Internet robert nusbickel
silver staining of DNA NEAL MUSTO
Membrane protein fusion vectors? Know of any? Paul Riggs
Antibody against neomycin phosphotransferase David S. Huen
Glycogen James Gibbs
oligos on membranes HOWARD at MGRP.BGSM.WFU.EDU
Ext. of DNA from agarose (which agarose) HARDIES at THORIN.UTHSCSA.EDU
rBGH organism Louis Hom
protein/nucleic acid motifs Dr. P.A. Crossey
Phosphoroimagers vs Instantimager Experiences Saught Brian Foley
beta-scanner Walt Lilly
SDS Problem Peter Heifetz
sections of hard tissues falling off HRMBDKC at HKUMD1.HKU.HK
Phosphoimagers vs Instantimager Roland J Saldanha
RT-PCR question Gerri Newfry
Aggregation of membrane proteins Dayanidhi Raman
RT-PCR question Sean David Moore
Phosphoimagers vs Instantimager Randall J. Cohrs
Promega on the Internet Peter Lundberg
Problem with Native PAGE Ding Ming
rBGH organism Roger Wiegand
Extraction of DNA from agarose gels Andre Hamel
Antibody against neomycin phosphotransfera Dan Rawlins Lab
5'->3' InhibitACE Andre Hamel
wanted: 'Simple Fool's guide to PCR' Dave Carmean
Rf: Aggregation of membrane proteins LOGAND
q Bianca L Torrez
RT-PCR question Richard R. Hardy
downward DNA transfer? Cornelius Krasel
receptor cloning Cornelius Krasel
dead cells Seth Corey
Phosphoimagers vs Instantimager Peter Heifetz
storing protein samples? **** Robert Slany
Phosphoroimagers vs Instantimager Experiences Saught Peter Heifetz
Capsaicin mhughes at mbcf.stjude.org
Which PCR machine? John Nelson
RT-PCR question Annette C. Hollmann
Best method for blunting ds DNA? Klaus Salger
Best Way Clone PCR Products Paul N Hengen
What's the best mini-prep? Paul N Hengen
Phosphoimagers vs Instantimager Tracy Aquilla
RT-PCR question Tracy Aquilla
Minipreps for ABI sequencer Robert Hindges
News Posting..eg/ Klaus.Matthaei at ANU.EDU.AU
Some Receptor Theory Questions...Constitutive or not? davis at wehi.edu.au
Type 52 Polaroid films? Who?****** David L. Haviland, Ph.D.
promega wizard kits and the 373 David L. Haviland, Ph.D.
Promega on the Internet Patrick Weix
ANTIBIOTIC STORAGE Martin Kennedy
In search of the "Holy manual" Timothy Bushnell
Oligo dT for RT? Elwyn Loh
In search of the "Holy manual" SPAGNOL at PLAUTO.CSATA.IT
membrane protein aggregation LOGAND
Address or phone needed Donna Leeper
Capsaicin Mike Morris
Rf: In search of the "Holy manual" LOGAND
SDS Problem john markwell
RT-PCR question douglas l feinstein
receptor cloning T. S. Pillay
storage of RNA NEAL MUSTO
retinol, cholecalciferol, vitamin B12 Stephen R. Lasky
promega wizard kits and the 373 WAS
Phosphoimagers vs Instantimager macIIci
chemistry of Qiaex beads & DNA interaction?? PRAMATHESH PATEL
Hot phenol prep, genomic DNA David Char
Single stranded DNA restriction?????? rnwil003 at uctvax.uct.ac.za
Which PCR machine? Darren Stauth
Extraction of DNA from agarose gels Wiggy
ANTIBIOTIC STORAGE David L. Haviland, Ph.D.
RNA hyb solns? JoeJoe
Phosphoimagers vs Instantimager Peter Heifetz
Best method for blunting ds DNA? David Matthews
Sequencing problem, HELP!! gli at UCDAVIS.EDU
Oligo dT for RT? POPOVA
Sequencing problem, HELP!! Glen Shearer
Taq left at RT - gulp! - Paul N Hengen
Which PCR machine? Dr. Greg Gloor
TaKaRa Single-Site Amplification David Johnson
Promega on the Internet Paul N Hengen
SSCP analysis protocol orbashu at ubvms.cc.buffalo.edu
Active Rb protein Ab Brett Levay-Young
In search of the "Holy manual" Cornelius Krasel
Antibodies to KDEL or kXXkx peptides Your Name Here
Tritium screens Richard
Cosmid sequencing with the 373 Lisa Bibbs
SSCP analysis protocol gli at UCDAVIS.EDU
Chemical cleavage of mismatches (CCM) Bill Oetting DGM-IHG
pcDNA3 with Wizard Paul N Hengen
beta-globin antibodies? Geoff Pinchbeck
ss. DNA restriction (yes, they cut) HARDIES at THORIN.UTHSCSA.EDU
Glowing BLUE DNA Gels Paul N Hengen
Which PCR machine? jfh
SDS problem (for bkg, not stringency) HARDIES at THORIN.UTHSCSA.EDU
RNA hyb solns? Glen Shearer
Invitrogen FTP/Gopher Site Christopher Hoover
Capillary Electrophoresis Todd Johnson
problem with native PAGE Nora Vig
chemistry of Qiaex beads & DNA interaction?? Shane Albright
qiagen column regeneration elias lolis
ABI sequencing and Stratagene Taq Extender Dirk Bornemeier
Do you have ABI Sequenase Kit problems??? Dirk Bornemeier
Glowing BLUE DNA Gels Klaus Salger
RNA from blood Richard Dana
PacI weirdness brett at BORCIM.WUSTL.EDU
Promega on the Internet WICKHAM,JOHN
Dye to colour DNA samples for PCR Shiao Y. Wang
Geiger Counter Satyam Subrahmanyam
In search of the "Holy manual" David S. Huen
RT-PCR question Shahram Mori
Seeking ELISA plate reader advice Donald Chun Kit Wong
Need help with Eczema Harold O. Leduc
UV-crosslinking of DNA-protien complex L.Y.Chau
protein precipitates:HELP ! Clemens Suter-Crazzolara
what is the best miniprep Sasha Kraev
Dye to colour DNA samples fro PCR Sasha Kraev
Rf: Antibodies to KDEL or kXXkx peptides LOGAND
Companies on the net Jonas Lindeberg
Wanted - buffer systems for pH range 4 to 9 in cell culture expt. Mr. P.A. Sansom
RT-PCR question Too Heng-Phon
Geiger Counter Paul A. Vescio
SUBSCIBTION H1411Mus at HUELLA.BITNET
sig John Wurzelmann
ANTIBIOTIC STORAGE MAD, MAD, MAD SCIENTIST
PCR from fungal colonies J Kennedy
Luciferase as internal control Graham Atherton
Kunkel mutagenesis- Help needed LEMAILLET
Disappearing retard band Graham Atherton
Thermus filiformis DNA polymerase Duncan Clark
is there an index, restr. enz. sources??? Robert Preston
ANTIBIOTIC STORAGE Andrew Cockburn
oligoscreening ? Joan Rigau Lloveras
Promega on the Internet Cornelius Krasel
Companies technical information supply online Bert Popping
anti-KDEL and anti-KxxKx antibodies LOGAND
Protein FPLC (HPLC) Systems Jim Litts
ANTIBIOTIC STORAGE Stephen_W._Mamber_at_~PRIWFB05 at ccmail.bms.com
In search of the "Holy manual" Frank Norman
Promega on the Internet: Biotechnet Frank Norman
Reticulocyte Lysate Stephen R. Lasky
Companies on the Net JEMERTON at BIOTECHNET.COM
is there an index, restr. enz. sources??? Klaus Salger
Beteine and inclusion bodies Rik Myers
What's the best mini-prep? B Stickney
Detection of rRNA methylation B Stickney
Dye to colour DNA samples for PCR Paul N Hengen
genomic dna prep Darin Obrien IHG
PHAGE DNA KITS? T. J. Murphy
DNA-affinity columns Alan Schoenfeld
Thermocyler runs JoeJoe
Non-denaturing Southern Blotting Scott J. Diede
Thermocyler runs Annette C. Hollmann
Beteine and inclusion bodies Daniel Mytelka
Companies on the Net Randall J. Cohrs
35S labeling E coli Paul Campbell
Pichia single-copy gene Nicholas Gascoigne
ANCHORED-PCR sl6dp at cc.usu.edu
Tris and DEPC B Stickney
35S labeling E coli Michael Benedik
Why MOPS for RNA gels? Glen Shearer
Thermocyler runs Robert Preston
test, sorry to bother Eric C. Anderson
GST fusion: Solution and more questions Rodney Tweten
UV-crosslinking of DNA-protien complex anon
WANTED: Old liquid scintillation counters Matthew J. Combs
Protein FPLC (HPLC) Systems Clifford Beall
cDNA Challenge************* Shahram Mori
RT-PCR question Shahram Mori
Single stranded DNA restriction?????? Shahram Mori
Biotrace membrane - Gelman Martin Kennedy
Promega on the Internet robert at chironex.jcu.edu.au
Phosphoimagers vs Instantimager Roland J Saldanha
protein precipitates:HELP ! Clemens Suter-Crazzolara
Methionine-free insect cell culture media Matthias Zeiner
Prokaryotic fusion protein systems Simon Dawson
RT-PCR question Simon Dawson
SDS Problem Simon Dawson
Companies on the net Rick Neubig
Seeking ELISA plate reader advice littgj at wmvx.lvs.dupont.com
PacI weirdness Cornelius Krasel
SDS Problem Clemens Suter-Crazzolara
keratinase / alkaline protease Tony Soltis
Growing E. coli George F Edick
>>>>URGENT : IN VITRO TRANSCRIPTION <<<<< Jim Owens
35 mm slides of gels - help Malcolm Moos Jr., M.D., Ph.D.
half life of GUS, luciferase and other reporters? Jeffrey Alan Silverman
half life of GUS, luciferase and other reporters? hraaxl at marc.cri.nz
MEK phosphorylation Jeffrey Alan Silverman
5' RACE Malcolm Moos Jr., M.D., Ph.D.
Sequencing insert from Lambda gt10 vector- HELP! Malcolm Moos Jr., M.D., Ph.D.
Biotrace membrane - Gelman JLars36920
clean GST antibodies source????? PRAMATHESH PATEL
test JPF jfield
clean GST antibodies source????? Matthias Zeiner
PostDoc Molecular Virology New Jersey (job) Vic Stollar
Kunkel mutagenesis- Help needed Jonas Lindeberg
Which PCR machine? Peter Doris
PHAGE DNA KITS? Randall J. Cohrs
dig labelled Riboprobe whole mount insitu? Dan Oerther
Hot phenol prep, genomic DNA enof
Sequencing YACS Martin Leach
Xenopus downtime Sherri Fraser
Best strategy from peptide sequences to cDNA clone? yqhuang at iastate.edu
PCR gene splicing: help! Kevin Morano
RT-PCR question rhubner at molbiol.ox.ac.uk
Storage of RNA rhubner at molbiol.ox.ac.uk
Why MOPS for RNA gels? Tracy Aquilla
Phosphoimagers vs Instantimager Tracy Aquilla
Electroelution and deglycosylation yqhuang at iastate.edu
Extraction of DNA from agarose gels Paul N Hengen
How do you measure rat's blood pressure??? Steve Konings
Frequently Asked Question (FAQ) list Paul N Hengen
Capsaicin Scott Selby Med Heme
How do you measure rat's blood pressure??? Stefan Kahlert
35S labeling E coli Arthur Hunt
Cleavage with NTCB Fabrice PARMENTIER LESAGE
Hot phenol prep, genomic DNA enof
PCR from fungal colonies enof
Info. on refrigerated microfuge Ramanujam Raman
Promega on the Internet Christopher Hoover
SDS problem Sasha Kraev
RDA Frank Cheng
Coupling IgG via sugar residues Dave Pearton
Seeking ELISA plate reader advice Donald Chun Kit Wong
PacI weirdness Duncan Clark
PacI weirdness Duncan Clark
Enough Re: Promega on the Internet Reinhard Doelz
non title 額賀路嘉
Coupling IgG via sugar residues POPOVA
35 mm slides of gels - help Ian A. York
Extraction of DNA from agarose gels Martin Leach
Need help With Blood RNA prep Richard Dana
Long range Genomic PCR jlanders
GST fusion: Solutions and more questions Ann Loraine
Best strategy pep. seq. -> cDNA HARDIES at THORIN.UTHSCSA.EDU
Why MOPS for RNA gels? NICHOLAS THEODORAKIS
Best method for blunting ds DNA? Annie Yang
qiagen column regeneration Annie Yang
Taq slippage in PCR Shiao Y. Wang
ANCHORED-PCR Shahram Mori
Coupling IgG via sugar residues Tomas Bratt
PCR gene splicing: help! Klas Kullander
Sequencing YACS Bruce Roe
Cycle Sequencing Question Bruce Roe
clean GST antibodies source????? T. S. Pillay
Kunkel mutagenesis- Help needed Yasha Hartberg
Idaho Technology RAPD analysis palasj9579 at cobra.uni.edu
PFGE and YACs Karl Fischer
subscribe gr8bn at cc.usu.edu
Electroelution and deglycosylation Prasad, Krishna
PFGE and YACs Elizabeth Rosenberg
Best method for blunting ds DNA? John J Welch
Motifs for txn factors? Dubear Kroening
PFGE and YACs Antje Haase
Kunkel mutagenesis- Help needed Berthe M. Willumsen
Why MOPS for RNA gels? D.Greeve <DAEMON>
Why MOPS for RNA gels? David S. Huen
Tritium screens / protein gels Berthe M. Willumsen
LacSwitch sequence Eivind Hovig
QUES: Galvanic Skin Response Todd G Gardner
cycseq question/YAC sequencing Sasha Kraev
Genesis Pharmaceuticals (Cambridge, MA) -- Combinatorial Chemistry ??? merlin
retroviral DNA preps Lorena C. Lim
refrigerated cent. colvin at ouvaxa.cats.ohiou.edu
retroviral DNA preps Olav Hungnes
PHAGE DNA KITS? Glen Shearer
Kunkel mutagenesis- Help needed Yasha Hartberg
PCR gene splicing: help! pskaytes at INTNET.UPJ.COM
PCR PRIMER DESIGN yu at ouvaxa.cats.ohiou.edu
Dictionary of Cell Biology on WWW ! Julian Dow
Aggregation of membrane proteins Andreas Buhr
Need address for Molecular Probes Inc. patel_p at bms.com
>>>Help about formal/agarose gel <<< CJF 90/10 INSERM
>>>Help about formal/agarose gel <<< Randall J. Cohrs
Clontech Ligation Express Richard T. Timmer
Long-Range PCR Mwatson at uniwa.uwa.edu.au
Single stranded DNA restriction?????? Mike Powell, PhD
Cheaper electroporation cuvettes? George W Chacko
Alsever's Solution henderson todd
Need address for Molecular Probes Inc. Stephen B. Aley
Best method for blunting ds DNA? Tom Chappell
ANNOUNCING: ALF DNA Sequencer Discussions in bionet.genome.chromosomes Jackson Shea
How annoying (was Re: RT-PCR question) Michael Poidinger
Capillary Electrophoresis equipment Todd Johnson
Wanted a mentor from industry Labuser 1
Why MOPS for RNA gels? Jim Astwood
Circular Dichroism and aggregates Mark Jason Logan
>>>Help about formal/agarose gel <<< Steve McKinney
Which PCR machine? Eric C. Anderson
Best strategy from peptide sequences to cDNA clone? Eric C. Anderson
Phosphoimagers vs Instantimager dhanzel at delphi.com
Optimisation of Hair Extraction Protocol Ciaran Meghen
Best strategy from peptide sequences to cDNA clone? Steve McKinney
south-western blot Dr Simminder Kaur
UV-crosslinking of DNA-protien complex Frances Hannan Zoology
(none) Zalman L. Suldan
Info. on refrigerated microfuge Frances Hannan Zoology
electroporation cuvettes Zalman L. Suldan
48-well plate tools? John E. Blume
long film & cassettes for sequencing - where? Frederick Garbrecht
PCR PRIMER DESIGN schupp at nauvax.ucc.nau.edu
PCR gene splicing: help! Greg Gloor
Optimisation of Hair Extraction Protocol Mike Morris
Cheaper electroporation cuvettes? szcooley at dale.ucdavis.edu
electroporation cuvettes szcooley at dale.ucdavis.edu
T4 ENDONUCLEASE VII URA1335 at ARTHUR.CITI2.FR
T4 ENDONUCLEASE VII ??? URA1335 at ARTHUR.CITI2.FR
hybridization bags Udo Schlosser, D1.4.03, Tel.: 6253
Expression problems Duncan Clark
microwave miniprep of plasmids S.A. Benson Lab
Cheaper electroporation cuvettes? Klas Kullander
PCR produced probes TIM ELLS
hybridization bags doug rhoads
EGF repeats in E.coli? Sam Henderson
(none) ITABAJARA DA SILVA VAZ JUNIOR
cold SSCP Daoust Martin
DNA FROM HAIR Todd Johnson
long film & cassettes for sequencing - where? anon
transcription+translation Peta C. Bonham-Smith
Canadian Novagen Users intermed at inforamp.net
hybridization bags patterson at frodo.mgh.harvard.edu
help ahmet arman
RACE, need help chien at mbcf.stjude.org
Viral Cell Packaging line GP+E86 POLAKBM at WPOGATE.SLU.EDU
Idaho Technology RAPD analysis Anthony V. Furano
cold SSCP gli at UCDAVIS.EDU
PC-based plasmid map programs nicola fidler
is there an index, restr. enz. sources??? Dana Macelis-Roberts Lab
>>> Help about formal/agarose ges <<< PHIL DANIEL
Quantitation of Westerns Jeanne Loring
sequence of pSP64(polyA) - Promega Robert.Coelen at jcu.edu.au
Recent DNA and RNA isolation methods wanted Richard Dana
LacZ in eukaryot. promoter studies Przemko
dig labelled Riboprobe whole mount insitu? P. Jones/ P. Scotting
Inverse PCR Dr. C.P. Healy
RNAse free water? CJF 90/10 INSERM
DNA purification Chutney
Polysomes preparation CJF 90/10 INSERM
non-rad colony screening Roger Kennett
Taq pol patent update an125249 at anon.penet.fi
Substitute for F12 K-media for HUVECs wanted aeschba at fmi.ch
help about formal/agarose gels slee at opal.tufts.edu
HELP: NEEDS A PIG rDNA CLONE opp at icbr
Info. on refrigerated microfuge Randall J. Cohrs
Inverse PCR Viraj Master
EXONUCLEASE III Michael Austin Roberts
Polysomes preparation Michael Coady
cDNA ()TPR1) wanted Thomas Deufel
Lambda clone isoln - Lambda fix Robert E Minto
Info. on refrigerated microfuge John Brunstein
Need reliable method for RNA extraction from lymphocytes Chris Hodgson
SSCP gels Donna Jensen
Buffer for plaque phage lysis --> PCR ? fgrun
microwave miniprep of plasmids K. Legate
FILTRATION ae55
enhancer dependent color Varyc
NaAc precipitation Sherri Fraser
Kits for Subtraction Libraries? John Storring
Why MOPS for RNA gels? Keith Hutchison
Cutting Edge (Was Re: Recent DNA and RNA isolation methods wanted) Michael Poidinger
Need reliable method for RNA extraction from lymphocytes Ben Vowels
Help:microwave miniprep of plasmids S.A. Benson Lab
Long range Genomic PCR Dave Adelson
Codon Usage and Expression in E.coli. vaughan at sci.wfeb.edu
35S-Met lifetime? Dave Adelson
hybridization bags Dave Pataky
Cancer Treatment Groups feco1104 at altair.selu.edu
0.02 filters CURTIS at UTMSI.ZO.UTEXAS.EDU
PCR journal Frederick Garbrecht
posting to bionet Michael Poidinger
6X myc cassette request Lorenzo Frigerio
FILTRATION K. Legate
Formiminoglutamate MURLEY,LAURA LEA,MS
Kits for Subtraction Libraries? John Nash
Mouse intestine cDNA library Richard Gomer
What's in Megascript buffer ? Patric Lundberg
Ambion RPA II Kit?? Jane Harrison
LacZ in eukaryot. promoter studies David S. Huen
PCR journal Kevin O'Donnell
Phage plaque PCR Sasha Kraev
dye in PCR Fabrice Glibert
protein precipitates:HELP ! Clemens Suter-Crazzolara
Inverse PCR? vdrover at kean.ucs.mun.ca
DNA purification Wiggy
Which PCR machine? G Gallagher
pulse field gels shelly may deane
Baculoviral Info: stephen marshalko
FILTRATION (K.LEGATE) ae55
Software to call heterozygosity from ABI output Ron Debry
GeneClean versus Quiagen Michael Sidler
HELP!!! Electroporation and PEG Alfredo Lopez
Info. on refrigerated microfuge B Stickney
Storage of RNA B Stickney
Promega on the Internet Helge Weissig
non-radioactive southerns C. McConville
freeze-sqeeze Daoust Martin
Sequencing vector Needed ! f.r.byrne
Pharmacia GeneQuant DNA calculator opinions wanted PENNY RIGGS
Codon Usage and Expression in E. coli Rebecca Schall
Direct sequencing for ABI 373 a.cox at rbgkew.org.uk
oligos kreuzer at bkfug.kfunigraz.ac.at
Reverse northerns,cDNA not hot enough. Robert McMaster
PCRE:PCR PRIMER DESIGN NEAL MUSTO
FILTRATION (K.LEGATE) K. Legate
What's in Megascript buffer ? MUNGO at UVVM.UVIC.CA
Ambion RPA II Kit?? Guilherme Co. Oliveira
Help!!! Removing polysaccharides from DNA preps Devaka Weerakoon
PC-based plasmid map programs Hank Seifert
subscripte bionet.molbio.methds-reagnts Danielle Nouaud
Olomoucine, a cdc2 Protein Kinase inhibitor? Mandy Johnstone
Why MOPS for RNA gels? Oleh Kevalchuke
GeneClean versus Quiagen CJF 90/10 INSERM
Buffer for plaque phage lysis --> PCR ? Stuart Brown
teflon tape Paul A. Vescio
freeze-sqeeze Paul N Hengen
luminous pen instead of radioactivity Stefan Gruenert (WCI)
formaldehyde deact. w/ glycine? JoeJoe
Storage of RNA Andre Hamel
teflon tape Randall J. Cohrs
Sequencing vector Needed ! Cornelius Krasel
Codon Usage and Expression in E. coli Cornelius Krasel
book Daoust Martin
GeneClean versus Quiagen Tracy Aquilla
EXONUCLEASE III Tracy Aquilla
5'- 3' Lauri Lintott
direct sequencing of PCR products MONTESCLAROS,LUZ,MISS
Luminous Pens Sherri Fraser
EXONUCLEASE III Klaus.Matthaei at ANU.EDU.AU
test troianovsk_s at msdisk.wustl.edu
GeneClean versus Quiagen Joseph Lipsick
luminous pen instead of radioactivity Joseph Lipsick
Luciferase as internal control John J Naleway
Promega on the Internet: Biotechnet John J Naleway
Companies on the Net John J Naleway
sequence of pSP64(polyA) - Promega Francis Ouellette
ECL direct Dieter Palmer
teflon tape Clemens Suter-Crazzolara
teflon tape (Dave Johnston)
ECL direct Dieter Palmer
Taq pol patent update an125249 at anon.penet.fi
XTT assay Geir Johannessen
What's in Megascript buffer ? Michael Coady
NaAc pptn Jeff Kramer
in situ PCR pattonaj%phvax.dnet at sb.com
protein expression - vessel size: summary XRDPROC at Gems.VCU.EDU
Need reliable method for RNA extraction from lymphocytes Howard T. Petrie
Need reliable method for RNA extraction from lymphocytes Howard T. Petrie
posting to bionet schupp at nauvax.ucc.nau.edu
Spin columns for DNA band excision? crawford at bscr.uga.edu
DNA purification schupp at nauvax.ucc.nau.edu
Spheroplast Philip Heacock
EXONUCLEASE III schupp at nauvax.ucc.nau.edu
Amino Acid Analysis software on www Uwe Hobohm
teflon tape JoeJoe
Qick nuclear extract preparation ? Jonas Lindeberg
ss DNA expression in Mammalian cells Dr. M.D. Jones
>>> Help about formal/agarose ges <<< David S. Huen
isolation of nuclei from lymphocytes michelle tourigny
GC-rich region Daoust Martin
Corvas International David Tuminaro
Codon Usage and Expression in E. coli Eric Carstens
E. coli lacZ complementation HERBERT WYCKOFF
PFGE and YACs Department of Research
Geiger Counter Peter T. Boag
Sequencing with degenerate primers Chris Upton
DNA-RNA hybrid cross-linking León Fernando García-Martínez
Syringe pump WANTED Lorne Taylor
nuclear proteins pattonaj%phvax.dnet at sb.com
luminous pen instead of radioactivity Dorothy Klein
EXONUCLEASE III Tracy Aquilla
luminous pen instead of radioactivity Bill Jack
tandem signal sequences Pak-Lam Yu
Requesting pGEM-luc and pSL301 daren Grossman - Pharmacology
Nonrad. ATP synthase assays? CConley
direct seq pcr w/abi eckloff at rcf.mayo.edu
Luciferase reporter gene with beta actin promoter x91holmen at wmich.edu
ss DNA expression in Mammalian cells g_wenck at spike.psc.scarolina.edu
ask for information Wenfu
Luciferase reporter gene/beta actin promoter x91holmen at wmich.edu
Urbino Meeting...info needed The doctor is IN.
direct sequencing of PCR products Karl Voss
Pharmacia GeneQuant DNA calculator opinions wanted Robert Woodward
luminous pen instead of radioactivity Robert Woodward
GC-rich region hschweiz
Stool RT-PCR Dale Yuzuki's Work Account
Method for Touchdown PCR Paiks
is there an index, restr. enz. sources??? Anita Gould
Storing PK\LDH without any sulfate ? Jean-Manuel Henry
Codon Usage and Expression in E.coli. Duncan Clark
DNA-Extr. of a Histological Cut H Rochholz/ H Haas
Baculoviral Info: Brad Romney
NaAc pptn. HARDIES at THORIN.UTHSCSA.EDU
GeneClean versus Quiagen Ian A. York
Best method of blunting DNA: SUMMARY and THANKS Bernard Murray
HRP substrate that fluoresces Rascals
Luminescent vs fluorescent ELISA James L Funderburgh
Expression problems WICKHAM,JOHN
Baculoviral Info: Cornelius Krasel
direct seq of PCR products Sasha Kraev
ss DNA expression in Mammalian cells Richard
Capillary Electrophoresis Robert Nelson
Need address for Molecular Probes Inc. Robert Nelson
protein precipitates:HELP ! Shane Albright
Topology of a multisubunit membrane protein Tuomas Haltia
XTT assay Dieter Palmer
Pharmacia GeneQuant DNA calculator opinions wanted Greg Denomme
luminous pen - use GLOGOS Tony Hodge
Yeast cDNA library kit Bert Popping
VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY Bruce James Ella
ATCC site Loryn Sellner
pRc/CMV containing lacZ Yoshiki Kawabe
E. coli lacZ complementation Duncan Clark
Codon Usage and Expression in E.coli. Duncan Clark
----URGENT : RNAase Free Water ?---- CJF 90/10 INSERM
---- Polysomes preparation ---- CJF 90/10 INSERM
Need address for Molecular Probes Inc. Sebastian W. Bunka
Ziplox - expressible? J Shepherd
GAL4 fusion/expression vectors ? Peter Uetz
Determination of genome size (Cot curves) Dr. C.P. Healy
Topology of a multisubunit membrane protein Flip Hoedemaeker
Maybe a stupid PCR question & a ETBr question mdcabl at cc.newcastle.edu.au
Re. direct seq of PCR products/Epicentre Kits Sasha Kraev
Non-radioactive run on Michele Fuortes
luminous pen instead of radioactivity Eric C. Anderson
c-myc expression system Peta C. Bonham-Smith
tandem signal sequences Pak-Lam Yu
Spin columns for DNA band excision? Howard T. Petrie
long ranger gels Peter Thorén
ANCIENT DNA Ciaran Meghen
luminous pen instead of radioactivity Leo Schalkwyk
ATCC site Neil Griffin
antibody to luciferase commercially available? G.E.Fazzi
ATCC site Shaun D. Black
ANCIENT DNA Chris Troy
PCgene program b137795 at mailserv.cuhk.hk
Small RNA and Polysome isolation LACYP at wsuvm1.csc.wsu.edu
S1 Nuclease kits Traci Roth
Sequencing with degenerate primers RichWalko
GC-rich region Richard Kenneth Bruce Martel
Kits for Subtraction Libraries? Kevin Pumiglia
Cheaper electroporation cuvettes? Eric Kofoid
(none) Anders Bäckström
pRc/CMV containing lacZ Martin Leach
ANCIENT DNA Tracy Aquilla
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" BAI_JN at JHUVMS.HCF.JHU.EDU
antibody to luciferase commercially available? Martin Leach
Help! DNA insoluble in water Nanfel Xu
Returned mail: User unknown lw75
Pharmacia GeneQuant DNA calculator opinions wanted Prenom Nom
Topology of a multisubunit membrane protein wegloehner at mpimg-berlin-dahlem.mpg.de
FILTRATION (Gelman Sciences) donnas at gelman.com
Sequencing with hexamers (was degenerate primers) Richard T. Pon
Need address for Molecular Probes Inc. Paquette Yves
Quickprep mRNA isolation from Pharmacia PEDP043 at uabdpo.dpo.uab.edu
lexA-antibody mingzi chen
problem with pUC18..help me !! Lee jang young
Codon preferences of differrent organisms Klaus Salger
RNA elution from agarose Oleh Kevalchuke
----URGENT : RNAase Free Water ?---- Klaus Salger
Help need for Single-step RNA Ioslation by Acid Guanidinium Randall J. Cohrs
Overexpression with pKK223-3 !! help Lee jang young
non-rad colony screening LizH277522
Volatile buffers info (TEAB; TEAA) Gregory Stuart
Reverse northerns,cDNA not hot enough. Dr. YL Du
long ranger gels Glen Shearer
HELP!!! Electroporation and PEG David L. Haviland, Ph.D.
Codon preferences of differrent organisms bio4 at ccnet.up.ac.za
Extraction of DNA from agarose gels leshof at delphi.com
Help! DNA insoluble in water Ian A. York
neosystem Michelle Chen
ANCIENT DNA Guy Hoelzer
NaAc precipitation anon
VectorNT1 experiences? adler at staff.monash.edu.au
densitometry software Terry Hanzlik
Hybaid products HRMBDKC at HKUMD1.HKU.HK
Announcement: 2 Hybrid System Review Available on MGH Gopher Server mendelsohn at Frodo.MGH.Harvard.EDU
E. coli lacZ complementation Roland J Saldanha
long ranger gels gustilo at pobox.upenn.edu
Green Fluorescent Protein J.Carnwath
Transfection of neuronal cell lines??? CARPENTER E.
Sequencing with degenerate primers Dave Smith
ANCIENT DNA Chris Troy
Artifacts in silver staining Bruce James Ella
Optimal Conditions for Boehringer PWO enzyme in PCR Jonathan Patrick Burke
anti-phosphoserine/threonine Ab? Jae-Won Soh
Hybaid products (Dave Johnston)
Artifacts in silver staining G.J.W.Euverink at biol.rug.nl
10 mers DAVID MITCHELL
synthetic proteins lw75
antibody to luciferase commercially available? Sheryl White
Acrylamide electrophoresis of DNA problem Albert
anti-phosphoserine/threonine Ab? Jim Woodgett
antibody to luciferase commercially available? Martin Leach
Transfection of neuronal cell lines??? Martin Leach
Artifacts in silver staining Frederick Garbrecht
Free BioTechniques Past Paper Database JEMERTON at BIOTECHNET.COM
cold SSCP parkef at delphi.com
Extraction of DNA from agarose gels Cornelius Krasel
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" dkim at unm.edu
synthetic proteins Cornelius Krasel
HIV-1 panel Varyc
teflon tape gc
RTPCR/degenerate primers with inosine? Cynthia Fisher
DNA insoluble in water HARDIES at THORIN.UTHSCSA.EDU
Transfection of oesophageal (squamous cell)carcinoma cell line Volkert Clausen
VOLATILE BUFFERS FOR COLUMN CHROMATOGRAPHY NICHOLAS THEODORAKIS
lexA-antibody
homocystamine (aminopropanethiol) Santhosh
teflon tape H. Rochholz/H. Haas
teflon tape Klaus Salger
PCgene program Gerri Newfry
ANCIENT DNA Tracy Aquilla
NruI Johannson Hans
Help! DNA insoluble in water anon
simple protocol for colony PCR Robert.Coelen at jcu.edu.au
PCR PRIMERS Efram Gonzalez
PC-based plasmid map programs Peter Gegenheimer
Marker for Plant? LEES at SASK.USASK.CA
Storage of RNA Peter Gegenheimer
simple protocol for colony PCR Anthony Palombella
cDNA library from wheat and soybean Richard Dana
EcR plasmid from Hogness at Stanford, CA Patric Lundberg
VOLATILE BUFFERS Bruce James Ella
Looking for a plasmid John McDougall
DIG system for Northern blotting Bruce James Ella
DNA from formalin preserved tissue Thomas F Duda
HOMOLOGOUS TRANSFECTION SYSTEM MUCHA JAN
immunoprecipitation Ralf Morgenstern
exonuclease I Walter Malangone
Macherey-Nagel Nucleobond for Seq.? LOGAND
HRP substrate that fluoresces littgj at wmvx.lvs.dupont.com
Luminescent vs fluorescent ELISA littgj at wmvx.lvs.dupont.com
nucleobond AX Jeff Kramer
homologous transfection system MUCHA at edv1.boku.ac.at
direct sequencing of PCR products Department of Research
exonuclease I Parke K. Flick
Yeast gene expression in mammalian cells Philip Heacock
Green Fluorescent Protein CARPENTER E.
hybridization bags Prasit Palitpolkanpim - GRSO
cDNA library from wheat and soybean Martin Leach
hybridization bags Genetic Phlegm
Co-transfection methods Ian A. York
dhfr antibody Min Xu
hybridization bags Ron Kagan
B.M.s 5 minute ligations? DAB
protein aggregation Timothy R. Hughes
Long gel dryers Frederick Garbrecht
antibody to luciferase commercially available? Sheryl White
DIG system for Northern blotting Klaus Salger
Why MOPS for RNA gels? Malcolm Moos Jr., M.D., Ph.D.
Buffer for plaque phage lysis --> PCR ? Malcolm Moos Jr., M.D., Ph.D.
Artifacts in silver staining Richard
Green Fluorescent Protein Paul N Hengen
Spin columns for DNA band excision? Mario de la Pena
RTPCR/degenerate primers with inosine? Robert.Coelen at jcu.edu.au
Riboprobe background hybridisation Klaus.Matthaei at ANU.EDU.AU
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" David J. Meyer
Buffering sat'd NaI sol'ns David J. Meyer
Bind-silane removal David J. Meyer
mutiple and faint bands in PCR Xin-Hua Zhou
Summary: simple protocol for colony PCR Robert.Coelen at jcu.edu.au
Help need for Single-step RNA Ioslation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction" Traci Roth
temperature-dependence of DNA-gyrase Dmitry A. Los
Hybaid products Sebastian W. Bunka
Quickprep mRNA isolation from Pharmacia MONSTER
simple protocol for colony PCR Sebastian W. Bunka
>>>Help about formal/agarose gel <<< S L Smithson
Long Ranger Gels Sasha Kraev
baculovirus and receptors megVx
pSV2dhfr seqeunce anyone? g_watson at icrf.icnet.uk
antibody database on the Net megVx
Expression problems Duncan Clark
Arabidopsis cDNA Iain Donaldson
temperature-dependence of DNA-gyrase Yasha Hartberg
Test,please ignore Fredrik Kamme
More PEG and Electrophoration Alfredo Lopez
formal/agarose gels Lauri Lintott
anti-phosphoserine/threonine Ab? T. S. Pillay
How good is the Eagle Eye II ? Alternatives ? Alan Lyons
pRc/CMV containing lacZ CARPENTER E.
Long Gel Dryers Sasha Kraev
anti-phosphoserine/threonine Ab? T. S. Pillay
NaAc precipitation Michael Cooley
Help me!! - prmoter inversion vector Lee, Jae-Hyun
problem with pUC18..help me !! Michael Cooley
Buffering sat'd NaI sol'ns Michael Cooley
DNA Amplification Market Robert Nelson
Codon preferences of differrent organisms Eric C. Anderson
Shotgun sequencing assembly Varyc
Long gel dryers Richard Kenneth Bruce Martel
Riboprobe background hybridisation Andre Hamel
>>>Help about formal/agarose gel <<< NICHOLAS THEODORAKIS
MATCHMAKER Two-Hybrid Supplement clontech!pauld at NETCOM.COM
NIH-3T3 drug test Jody Groenendyk
ELISA microplate reader Donald Chun Kit Wong
use of sodium salicylate prior to autoradiography hschweiz
>>>Help about formal/agarose gel <<< Bernard Murray
staining for beta-galactosidase BMA14432 at vax1.utulsa.edu
looking for calpain antibody Robert Neumar
Long gel dryers Jonas Lindeberg
Long gel dryers Klaus Salger
Transfection of neuronal cell lines??? Michael Poidinger
Homebrew saline for Contact Lenses Robert Rumpf
Buffering sat'd NaI sol'ns Virginia Dress
Oligonucleotides as Linkers? Weiss
pCI lee shekter
E. coli lacZ complementation mike
ammonium sulfate precipitation of proteins bipin dalmia
wizard prep of DNA gibbs at sbsu1.auckland.ac.nz
Adding membrane signal to cytoplasmic protein ORSULIC at UNCVX1.OIT.UNC.EDU
southern and northern on the same blot hrichter at ulys.unil.ch
DNA Ladders for Short Fragments Guy Hoelzer
NuSieve Agarose Michael Gieseg
spectrophotomer Therese Ann Dietlin
Help needed with alkaline phosphatase conjugation to oligonucleotides Dominic Voon
Inosine-containing oligos Grace Mendoza
Long gel dryers MBDMITRY at CCVAX.SINICA.EDU.TW
Homebrew saline for Contact Lenses Loryn Sellner
Why dATP ? MONSTER
Quantitation of Westerns dhanzel at delphi.com
non-radioactive southerns dhanzel at delphi.com
Luminescent vs fluorescent ELISA dhanzel at delphi.com
Need address for Molecular Probes Inc. dhanzel at delphi.com
EtBr-again KARI KAMMIOVIRTA MMKAB
Free BioTechniques Past Paper Database Lee, Jae-Hyun
NuSieve Agarose Mike Morris
Pseudomonas/bacteriocins FJ van der Wal
Free BioTechniques Past Paper Database Cornelius Krasel
ammonium sulfate precipitation of proteins Cornelius Krasel
anti-Taq polymerase antibodies for hotstart RMARTINQFD at BIOTECHNET.COM
temperature-dependence of DNA-gyrase christophe gilbert
pRc/CMV containing LacZ g_watson at icrf.icnet.uk
Diagnostics Prototype Newsgroup Kevin O'Donnell
Free BioTechniques Past Paper Database J.Carnwath
Plasmid copy number jim hartley
pRc/CMV containing lacZ Klas Kullander
NIH-3T3 drug test Klas Kullander
anti-Taq polymerase antibodies for hotstart J.Carnwath
address for Dynatech? J.Carnwath
ammonium sulfate precipitation of proteins Flip Hoedemaeker
Alk phos conjugation Varyc
Companies on the Net walling at wmvx.lvs.dupont.com
Inosine-containing oligos John E. Fox
ammonium sulfate precipitation of proteins larson eric
B.M.s 5 minute ligations? David Micklem
What is the perfect PCR? John Dixon
Long gel dryers (Dave Johnston)
Free BioTechniques Past Paper Database JEMERTON at BIOTECHNET.COM
address for Dynatech? Klas Kullander
Why dATP ? Pete M. Muriana
EtBr-again Yasha Hartberg
Homebrew saline for Contact Lenses Brad Riesenberger
Transfection of neuronal cell lines??? CARPENTER E.
Inverse PCR Dr. C.P. Healy
ELISA plates and protein adsorbtion Emanuele Buratti
Plasmid copy number control jim hartley
Transfection of CaCo-2 cells Ane Gedde-Dahl
Maybe a stupid PCR question & a ETBr question Darren Stauth
cDNA kits? Greg Gloor
Plasmid copy number control bchs1b
Free BioTechniques Past Paper Database Carl Lawyer
Shotgun sequencing assembly Peter Myler
staining for beta-galactosidase Michael Poidinger
test Charlie Spillane
Q: Human cDNA libraries? Mike Morris
anti-Taq polymerase antibodies for hotstart Carolyn M Wetzel
protease info / database Gene Holowachuk
EtBr-again Klaus Salger
wizard prep of DNA MAD, MAD, MAD SCIENTIST
What is the perfect PCR? Stuart Brown
DNA sequencing services cote at bscr.uga.edu
long run sequencing Sasha Kraev
PCgene program Hank Seifert
Detergents and Disruption Louis Hom
Question on generating nicked circular DNA using EthBr and EcoRI Rock Pulak
Maybe a stupid PCR ques Margaret Shirra
ECL for colony hybridization Rinkei Ko
luminous pen instead of radioactivity dkatz at doane.edu
Where to buy genomic libraries? Tracy Aquilla
Where to buy genomic libraries? Martin Leach
TIGR or HGS address? jfh
Enzymes in KGB? Klaus Salger
Long gel dryers Klaus.Matthaei at ANU.EDU.AU
Riboprobe background vector Klaus.Matthaei at ANU.EDU.AU
DNA from formaldehyde preserved specimens JohnS52765
Homebrew saline for Contact Lenses Shawn C. Baker
oligonucleotide synthesizers hl15 at hubrecht.nl
Inverse PCR TIM ELLS
ECL for colony hybridization Pete Muriana
HRP substrate that fluoresces John J Naleway
Luminescent vs fluorescent ELISA John J Naleway
Marker for Plant? John J Naleway
staining for beta-galactosidase John J Naleway
suggestion: include email address in publications merlin
oligonucleotide synthesizers Roland J Saldanha
Home made glass FOG/MIST request Chris Upton
direct sequencing of PCR products Steve Marsh
Maybe a stupid PCR question & a ETBr question Andre Hamel
Homebrew saline for Contact Lenses Eric R. Hugo
gene targeting -- homologous recombination -- why not liposomes? merlin
suggestion: include email address in publications Pete Muriana
? Supplier of mRNA Diff. display kit T. S. Pillay
Tyrosine kinases in bacteria T. S. Pillay
(none) S.Mitchell Halloran
Question on generating nicked circular DNA using EthBr and EcoRI Zophonias O. Jonsson
Macherey-Nagel Nucleobond for Seq.? Frank White
Help-plasmid recovery from dead plate! Tara McCready
Maybe a stupid PCR question & a ETBr question Klaus.Matthaei at ANU.EDU.AU
Nonrad. ATP synthase assays? Brian T. Greuel
PCgene program Benoit_Hebert at IAF.UQUEBEC.CA
DNA* LEES at SASK.USASK.CA
Homebrew saline for Contact Lenses Loryn Sellner
DNA/phenol problem Jennifer_ Welsh
oligo(dT) with single base at 3'-end... rhubner at molbiol.ox.ac.uk
Maybe a stupid PCR ques Fabrice Glibert
Long gel dryers Jim Owens
DNA* Carl Lawyer
protein aggregation FORSDYKE at QUCDN.QueensU.CA
Help: Program to find quasi-restriction sites Juan Antonio Contreras
detection of 5-methyl cytosine (bisulfite method) Onno Myriam
detection of 5-methyl cytosine (bisulfite method) Onno Myriam
TOMATO cDNA library LEMAILLET
What is the perfect PCR? Agnieszka Szczepek
Enzymes in KGB? Carlisle Landel
isotec ITABAJARA DA SILVA VAZ JUNIOR
Problems w/ native PAGE Paul A Bucciaglia
Chicken typeII collagen probe Dr. C.P. Healy
DNA/phenol problem Darren Stauth
detection of 5-methyl cytosine (third question)) Onno Myriam
PCgene program Peter Myler
DNA* Peter Myler
Free sulfhydryl determination Shaun D. Black
pIN-III-ompA-hind Dr. Jon R Sayers
cDNA library screens via PCR Lynn M Heltemes
stimulation of BT-20's & MCF-7 cells Philip Joseph Moos
help: protocol for removal of dye terminators for cyc. seq. John Williford
Dynatech address Tahilyamani at pahkr6.Syntex.Com
? Supplier of mRNA Diff. display kit Patrice Ferriola
Ghosts and membrane fluidity Jan Palaty
Homebrew saline for Contact Lenses Donald A. Lehn, Ph.D.
? Supplier of mRNA Diff. display kit RICHARD P. PHIPPS
kit for differential display pattonaj%phvax.dnet at sb.com
Detection of "Invisible" Proteins??? Karl Fischer
isotec Clifford Beall
urinary iodine determn; water qual. anal. with cap. elect. S.Mitchell Halloran
DIG system for Northern blotting Elizabeth Estabrook
Problems w/ native PAGE POPOVA
Inverse PCR Martin Leach
PCR fidelity: Calculations? Brian Foley
Sal I cloning problems Lisa Taylor
non-radioactive DNA sequencing MARIA ELENA GARCIA DIAZ
nuclear melanin Louis Hom
Detection of "Invisible" Proteins??? ggordon at opal.tufts.edu
Quick response from Hogness lab! Patric Lundberg
Problems w/ native PAGE Yasha Hartberg
gene targeting -- homologous recombination -- why not liposomes? Martin Kennedy
ECL for colony hybridization dpetersn at unixg.ubc.ca
ANNOUNCE: USED ANALYTICAL LAB EQUIPMENT FOR SALE John Brookes
Detection of "Invisible" Proteins??? Ding Ming
Dimerization of plasmid in mini-preped sample? Jinsuk Kim
Detection of "Invisible" Proteins??? - Reply Varyc
XL-PCR ? Jonas Lindeberg
error free proteins Varyc
Wanted Japan/uk fellowship candidate P. Jones/ P. Scotting
cDNA kits? Stephen R. Lasky, Ph.D. alt.binaries.pictures.erotica.blondes alt.binaries.pictures.erotica.d alt.binaries.pictures.erotica.female alt.binaries.pictures.erotica.male alt.binaries.pictures.erotica.orientals alt.binaries.pictures.fine-art.d alt
curing bacteria of plasmids Candace Krepel
Protein Purification - Woe is me!!! Andy Hill
DNA extraction: Lactob. Heitor Luiz da C. Coutinho
error free proteins Roger Wiegand
MSE Centrifuges (Help!) mlrjsg at dct.ac.uk
Protein Purification - Woe is me!!! Curt Ashendel
internet for the mol. biologist GRIFFINHG
native gel Todd Johnson
curing bacteria of plasmids Pete Muriana
HELP: NORTHWESTERN ASSAY gr8bn at cc.usu.edu
Radiographic Duplicating Unit Steven Hecht
cDNA library screens via PCR Sandra Munro
32P labeling tumors in vivo Gene Holowachuk
Tyrosine kinases in bacteria Jim Woodgett
Need RNA structure program slee at opal.tufts.edu
KLENTAQ manufacturer jchurch at kean.ucs.mun.ca
ABRF network NISSON at BIOTECHNET.COM
Dimerization of plasmid in mini-preped sample? benedik at uh.edu
DNA/phenol problem MAD, MAD, MAD SCIENTIST
non-radioactive DNA sequencing LKM100F at ODUVM.BITNET
Radiographic Duplicating Unit kramerse at wmvx.lvs.dupont.com
Detection of "Invisible" Proteins??? Jon Chappel
Nonrad. ATP synthase assays? Ferenc Marincs
DEPC treated water in PCR AARON J. MOE
internet for the mol. biologist Martin Leach
internet for the mol. biologist Martin Leach
PCR problems mwatson at uniwa.uwa.edu.au
Detection of "Invisible" Proteins??? NICHOLAS THEODORAKIS
(none) Bruce J. Turner
incorporating biotin-dNTPs schoenfeld at MSSCC.MED.UTAH.EDU
DEPC treated water in PCR John W. Longshore
long ranger gels Mic Chaudoir
ANCIENT DNA Mic Chaudoir
Cheaper electroporation cuvettes? Mic Chaudoir
Homebrew saline for Contact Lenses Mic Chaudoir
RT-PCR in NuSieve ??? Michael Deyholos
Nonrad. ATP synthase assays? Ferenc Marincs
simple protocol for colony PCR Ferenc Marincs
RNA from bacteria Ferenc Marincs
GST Surasakdi Wongratanacheewin
Long gel dryers mhughes at mbcf.stjude.org
Adding membrane signal to cytoplasmic protein mhughes at mbcf.stjude.org
RNA Isolation Robert Nelson
Detection of "Invisible" Proteins??? Bernard Murray
YY1 Tan Shyh Han
methylmercuric hydroxide Azlinda Bte Anwar
protease cleaving Ser-Ala M.A.Schmidt
pRc/CMV containing lacZ Dave Smith
Denatured DNA and fixation Frank Karlsen
Fixation of DNA close to protein Frank Karlsen
electroporation Yoshiyuki Shibata
Plasmid collections-where? Dr. Jon R Sayers
E. coli lacZ complementation Chris Boyd
Contamination in Nested PCR Patty McManus
PCR of GC-rich genes sbaker at molbiol.ox.ac.uk
Ecoli periplasmic secretion vectors? mbsay at seqnet.dl.ac.uk
get rid of developer stains from clothing AMBION at DELPHI.COM
How good is the Eagle Eye II ? Alternatives ? mcgeed at HAL.HAHNEMANN.EDU
Contamination in Nested PCR Martin Leach
Electroporation cuvettes mcgeed at HAL.HAHNEMANN.EDU
COT 1 preparation! HELP!!! David S. Huen
internet for the mol. biologist Francis Ouellette
internet for the mol. biologist Francis Ouellette
chaps nussbaum at neurochem.u-strasbg.fr
internet for the mol. biologist Francis Ouellette
PCR of GC-rich genes John W. Longshore
SS amplification woes VICDEF at BIOLOGY.BIOSCI.WAYNE.EDU
test Cheng Luo #3295
RNA structure graphics software Jerry Grandoni
DNA/phenol problem Chuck Cerveny
DNA/phenol problem Stuart Kuhstoss
Fluorometer Muhammad Lodhi
Detection of "Invisible" Proteins??? Torben Osterlund
PCR of GC-rich genes Clifford Beall
Sal I cloning problems rpgrant at molbiol.ox.ac.uk
How good is the Eagle Eye II ? Alternatives ? dallas at mcvax4.d48.lilly.com
Ellman's Reagent/DTNB (PROTOCOL) Shaun D. Black
PCR of GC-rich genes doug_mckenzie at stratagene.com
? Supplier of mRNA Diff. display kit David Glover
RT storage? croyleml at ucbeh.san.uc.edu
GST Bernard Heymann
How good is the Eagle Eye II ? Alternatives ? PMacklon
Does no polyadenylation site mean no polyadenylation? rr at BMMB01.UMESCI.MAINE.EDU
Detection of "Invisible" Proteins??? Virginia Dress
KLENTAQ manufacturer kamin johnson
PCR crash PANIAGUA SOLIS JORGE FERNANDO
Measuring Plant Cell Death cote at bscr.uga.edu
DNA/phenol problem Andre Hamel
PCR crash Kevin Morano
GST T. S. Pillay
urea/SDS, guanidine/SDS PAGE Patric Lundberg
Immobilon CD membranes- do they work? T. S. Pillay
help! purifying polyclonals BARKER, SHARON L.
KLENTAQ manufacturer In C. Kim
Operon (?) Inc. - need Address etc... Mandalaparthy
Anyone using "hot start" cycle sequencing? gustilo at pobox.upenn.edu
urea/SDS, guanidine/SDS PAGE HSchreiner
What's the best condition for transfection of most commonly used cell lines? Aurel d'etre
need protocol for pcr of phage from plates. Mandalaparthy
Bulk prep problems Michael Gieseg
help for oligo hybridization Limin Li
Which Kit? MONSTER
Were we can buy cheapest DNA primers? KIMMO KOIVU MMKAT_OPISK
Were we can buy cheapest DNA primers? KIMMO KOIVU MMKAT_OPISK
Sal I cloning problems Jonas Lindeberg
mycobact databases Varyc
Vistra frits at hubrecht.nl
Strength of 4ml Spec. Cuvettes Nigel Eastmond
RNA structure graphics software Cornelius Krasel
Idaho Technology Air Thermo-Cycler... Any good? Peter Thorén
Adding membrane signal to cytoplasmic protein George W Chacko
Adding membrane signal to cytoplasmic protein Kevin Pumiglia
Enzymes in KGB? Duke Groebe
Single lane/column sequencing-help rnwil003 at uctvax.uct.ac.za
Adding membrane signal to cytoplasmic protein Shaun D. Black
Rf: Strength of 4ml Spec. Cuvettes LOGAND
Bioventures DNA isolation Kit MAD, MAD, MAD SCIENTIST
oligo hybridization Bruce J. Turner
RoboCycler? dudy bar-zvi
Does no polyadenylation site mean no polyadenylation? Clemens Suter-Crazzolara
plasmid dimerization Sasha Kraev
urea/SDS, guanidine/SDS PAGE Curt Ashendel
temperature-dependence of DNA-gyrase Steve Minchin
PCR crash Clemens Suter-Crazzolara
HA epitope tag problems PJOB
DNA/phenol problem Michael Cooley
Fluorometer Michael Cooley
Subscription Slawek Dabrowski
Adding membrane signal to cytoplasmic protein Cornelius Krasel
Electron microscopy of Plasmid Ali Karami
Anyone using "hot start" cycle sequencing? Darren Stauth
(none) Anders Bäckström
RNA isolation out of OCT embedded sections? Harry Menegay
Keystone Oncogenes mtg: Want Roommate Steve Eklund
txn factor consensus seq. database?? Jeffrey H. Boatright
address of So. Cal forensic lab Walter Hill
oligonucleotide synthesizers John E. Fox
urea/SDS, guanidine/SDS PAGE gflentke at facstaff.wisc.edu
Assymetric PCR references Ashok Aiyar
QUICKHYBE from Stratagene - opinions? steven joseph chmura
urea/SDS, guanidine/SDS PAGE Song Tan
KLENTAQ manufacturer tebas_p at msdisk.wustl.edu
Keystone Oncogenes mtg: Want Roommate seklund.internet{seklund at netserv.unmc.edu}
Extraction of DNA from Agarose Gel Laurence T. Reiter
silica based DNA extraction Jinghai Xu
urea/SDS, guanidine/SDS PAGE gflentke.internet{gflentke at facstaff.wisc.edu}
DNA/phenol problem Jim Owens
Adding membrane signal to cytoplasmic protein Michael Coady
Keystone Oncogenes mtg: Want Roommate seklund.internet{seklund.internet{seklund at netserv.unmc.edu}
silica based DNA extraction jxu.internet{jxu at MIT.EDU}
Assymetric PCR references Martin Leach
Extraction of DNA from Agarose Gel anon
QUICKHYBE from Stratagene - opinions? sjchmura.internet{sjchmura at kimbark.uchicago.edu}
txn factor consensus seq. database?? jboatri.internet{jboatri at eagle.cc.emory.edu}
oligonucleotide synthesizers altabios.internet{altabios at bham.ac.uk}
address of So. Cal forensic lab walterh.internet{walterh at u.washington.edu}
Human cell transformation- SOD1 and SOD2 genes Brad Wouters
HELP: NORTHWESTERN ASSAY John J Weiland
Ab for 6XHis tag?? Sam Henderson
Does no polyadenylation site mean no polyadenylation? Steve McKinney
Does no polyadenylation site mean no polyadenylation? Martin Kennedy
help for oligo hybridization Timothy Bushnell
help for oligo hybridization Gabriel Romero
need protocol for pcr:gt10vector, frm plaques. Mandalaparthy
How to Purify Glyc. Dehyd? Dave Bremer
Test Message - Don't read! Peter Mulderry
Extraction of DNA from Agarose Gel T. S. Pillay
CAT assays henderson
Bioventures DNA isolation Kit Rebecca Schall
minipreps for sequencing Varyc
HA epitope tag problems K. Legate
Bioscience Archives? jnewton at molbiol.ox.ac.uk
QUICKHYBE from Stratagene - opinions? Duke Groebe
Ab for 6XHis tag?? Clemens Suter-Crazzolara
subcloning lamda library Lauri Lintott
Transfection of oesophageal (squamous cell)carcinoma cell line Irene Schauer
Amplification of pACYC184 plasmid jalexand at crihan.fr
TA Vector jnewton at molbiol.ox.ac.uk
CAT assays Martin Leach
inverse PCR & the price of anchovies B.W. Wren
DISCUSSION - plasmid piracy = software piracy ? Martin Leach
inverse PCR & the price of anchovies B.W. Wren
Desperation - how do I get off? dacoj3 at wptemp.kvl.dk
Electroporation cuvettes mcgeed at HAL.HAHNEMANN.EDU
Denatured DNA and fixation Guy Hoelzer
Electroporation cuvettes mcgeed at HAL.HAHNEMANN.EDU
Detection of "Invisible" Proteins??? - Reply Tom Chappell
Cheaper electroporation cuvettes? Heather H Lord
random priming braam at BIOC.RICE.EDU
inducible drug resistance JKHLab
TA Vector Martin Leach
Quantitative protein determinations in plant extracts bipin dalmia
cloning-lambda-inserts Lauri Lintott
cloning-lambda libraries Richard Kenneth Bruce Martel
ab:6xHisTag AKravetz
minipreps for sequencing AKravetz
random priming Martin Leach
HA epitope tag problems Jim Woodgett
Problems w/ native PAGE Paul A Bucciaglia
minipreps for sequencing Thomas Newman
Westerns to detect HA Tag? ToddS41
Glowing BLUE DNA Gels fnord
Protocal for DNA extension Alex Chang
hemagglutinin epitope for Westerns ROSS at SCI.WFEB.EDU
request advice on RNA storage Robert Hewitt
request advice on mRNA preparation Robert Hewitt
request advice on mRNA preparation Klaus Salger
Epitopes for prokaryotic proteins Kah-Tong, Seow
DISCUSSION - plasmid piracy = software piracy ? Alex Knight
pGRE5.1, the inducing condtion Shu-Chih Chen
Primers NOT needed for RT rx'n?!?! Andre Hamel
Smallest Cloning Vector and Selectable Marker ... Allin Verreault in Stillman lab
check mail. ranga at vigyan.iisc.ernet.in
Bulk prep problems Duncan Clark
hot start in PCR(cycle seq) Sasha Kraev
Smallest Cloning Vector and Selectable Marker ... Clemens Suter-Crazzolara
DISCUSSION - plasmid piracy = software piracy ? schupp at nauvax.ucc.nau.edu
Amplification of pACYC184 plasmid schupp at nauvax.ucc.nau.edu
measuring peptide concentration-exactly! STRUCTURAL NEUROBIOLOGY LAB
Were we can buy cheapest DNA primers? Robert Nelson
PCR crash Robert Nelson
DISCUSSION - plasmid piracy = software piracy ? Chris Upton
Homemade GeneClean kits ??? fnord
test David Hosfield
subscription JOHN A. KORTE
betagalactosidase antibodies David Gonda
oligo-binding membranes HOWARD at MGRP.BGSM.WFU.EDU
Transient transfection assays. Laurence T. Reiter
Homemade GeneClean kits ??? Marc Goldstein
Need Advice on Film Recorders gustilo at pobox.upenn.edu
Info on Dynal magnetic beads (efficiency of extraction) ajayaram at pearl.tufts.edu
Bulk prep problems Martin Kennedy
Epitopes for prokaryotic proteins wang qi
Western Using Monoclonals wang qi
RNA from paraffin embedded tissue Loryn Sellner
Green Fluorescent Protein J.Carnwath
TRANSFAC database Edgar Wingender
Summary: PCR of GC-rich genes sbaker at molbiol.ox.ac.uk
DIG-labelled antisense for RNAse protection? Jose Nieto Path
Were we can buy cheapest DNA primers? John E. Fox
DISCUSSION - plasmid piracy = software piracy ? Duncan Clark
Western Using Monoclonals Frederick Garbrecht
PCR problems Sean David Moore
HA epitope tag problems Anne Spang
DISCUSSION - plasmid piracy = software piracy ? Randall J. Cohrs
Idaho Technology Air Thermo-Cycler... Any good? Viraj Master
Western Using Monoclonals Anne Spang
Luciferase Problems? Sherri Fraser
inducible drug resistance JKHLab
Luciferase Problems? Martin Leach
Luciferase Problems? sfraser.internet{sfraser at acs.ucalgary.ca}
Western Using Monoclonals Annette C. Hollmann
PCR crash Emma Macfarlane
Genomic DNA from E.coli without phenol or chloroform?? Gabriel Dorado
Anti-LexA protein antibodies?? Dennis Templeton
Advse needed on DNA strand-separation Baohua Huang
Stability of Heparin Ramanujam Raman
westerns using monoclonals pattonaj%phvax.dnet at sb.com
AUSTRALIA, Sidney, St. Vincent's Hospital, Dr. J.A. Eisman et al?? Gabriel Dorado
Bulk prep problems (Yes, plasmids can integrate) HARDIES at THORIN.UTHSCSA.EDU
pCMV5 sequence ? Roger Graham
Methylmercuric Hydroxide Christopher Hoover
txn factor consensus seq. database?? Brian Foley
QUESTIONS ON PCR CONDITIONS W/ DEGENERATE PRIMERS Stephen M. McCrain
How good is the Eagle Eye II ? Alternatives ? Mic Chaudoir
help for oligo hybridization Mic Chaudoir
Western Using Monoclonals Mic Chaudoir
screening plasmid libraries Yuanhao Li
Designing Ideal PCR Primers In C. Kim
Advice on Film Recorders Mike Kitamura
Need Advice on Film Recorders RNeubig at umich.edu
Spectrophotometer Help Andrew McArthur
screening plasmid libraries Jon Burrows
minipreps for sequencing Stephane Vuilleumier
CAT assays Traci Roth
Smallest Cloning Vector and Selectable Marker ... Scott B. Mulrooney

Last message date: Mon Oct 31 23:05:18 EST 1994
Archived on: Sun Mar 6 21:26:25 EST 2005

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