Tnt Sysetem could not work,help.

Shuichi Oka imamura at
Thu Sep 1 00:35:05 EST 1994

Dear all
Does anyone have experience with doing Tnt system (T7 Promotor) ? I'm
currently trying to  do in vitro translation by using the Tnt system (T7
Promotor) .It  work in very well with  positive control vector in the kit
,but not with my construct vector  ( in pT7 blue : Novagene :  This 
sequense is perfect.).  Comments from the ones have succeded in such system
trial are welcomed.

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