RNA isolation from small # of cells?

Paul Scotting mbzpj at unicorn.nott.ac.uk
Thu Sep 1 08:08:25 EST 1994


In article <1994Aug29.142820.43973 at yogi>, bormann at urz.unibas.ch wrote:

> Hi Bionetters,
> 
> I would like to isolate RNA from a small number of cells
> (approx. 500) to finally make a library.
> Does anybody have a suggestion or experience how to do
> this?   Thanks a lot.
> 
> Peter.


I have had success using RNAzol to isolate RNA from a few sections of
frozen tissue. In this case I used a relatively large volume of RNAzol,
about 1ml. Since the RNAzol protocol is so simple the volumes could easily
be reduced according to sample size. Good luck.
                      Paul



More information about the Methods mailing list