RNA isolation from small # of cells?

Paul Scotting mbzpj at unicorn.nott.ac.uk
Thu Sep 1 08:08:25 EST 1994

In article <1994Aug29.142820.43973 at yogi>, bormann at urz.unibas.ch wrote:

> Hi Bionetters,
> I would like to isolate RNA from a small number of cells
> (approx. 500) to finally make a library.
> Does anybody have a suggestion or experience how to do
> this?   Thanks a lot.
> Peter.

I have had success using RNAzol to isolate RNA from a few sections of
frozen tissue. In this case I used a relatively large volume of RNAzol,
about 1ml. Since the RNAzol protocol is so simple the volumes could easily
be reduced according to sample size. Good luck.

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