RNA isolation from small # of cells?
mbzpj at unicorn.nott.ac.uk
Thu Sep 1 08:08:25 EST 1994
In article <1994Aug29.142820.43973 at yogi>, bormann at urz.unibas.ch wrote:
> Hi Bionetters,
> I would like to isolate RNA from a small number of cells
> (approx. 500) to finally make a library.
> Does anybody have a suggestion or experience how to do
> this? Thanks a lot.
I have had success using RNAzol to isolate RNA from a few sections of
frozen tissue. In this case I used a relatively large volume of RNAzol,
about 1ml. Since the RNAzol protocol is so simple the volumes could easily
be reduced according to sample size. Good luck.
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