primers with dI vs mixed bases

Emma Macfarlane emma at HERA.MED.UTORONTO.CA
Thu Sep 1 09:58:49 EST 1994

Hi Allen
I recently used a similar strategy to pcr up a lactate dehydrogenase 
gene and used degenerate oligos with inosine in 3 to 4 positions.
It worked beautifully. I didn't try primers with mixed bases for 
this experiment for comparison but have used them for others and
have never had such a clean result - this could be due to the experiment
and not the primers however....
Personally I would go with the inosine
Good luck

More information about the Methods mailing list