primers with dI vs mixed bases
emma at HERA.MED.UTORONTO.CA
Thu Sep 1 09:58:49 EST 1994
I recently used a similar strategy to pcr up a lactate dehydrogenase
gene and used degenerate oligos with inosine in 3 to 4 positions.
It worked beautifully. I didn't try primers with mixed bases for
this experiment for comparison but have used them for others and
have never had such a clean result - this could be due to the experiment
and not the primers however....
Personally I would go with the inosine
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