MW Markers for RNA gels...

M.J. Principe u8908594 at muss.cis.McMaster.CA
Thu Sep 1 16:01:08 EST 1994


In article <00983D38.375E2780.230 at umbsky.cc.umb.edu>,
SPIKE <5978jsnyd at UMBSKY.CC.UMB.EDU> wrote:
>
>I will be running in vitro transcripts out on polyacrylamide gels and need to
>use molecular weight markers. How accurate is it to end-label DNA markers with
>polynucleotide kinase and 32P-ATP and use those?  Do I heat them first to 
>denature them?  Are there better methods?  Thanks in advance for your help!
>
What we do is use cold pre-stained markers, and prior to exposing them to 
the film, we dot them with a fountain pen containing ink and tailings 
from old 32P tubes. Makes for a radioactive ink, which we cover with 
Scotch tape (to prevent smudging on the film).





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