MW Markers for RNA gels...
u8908594 at muss.cis.McMaster.CA
Thu Sep 1 16:01:08 EST 1994
In article <00983D38.375E2780.230 at umbsky.cc.umb.edu>,
SPIKE <5978jsnyd at UMBSKY.CC.UMB.EDU> wrote:
>I will be running in vitro transcripts out on polyacrylamide gels and need to
>use molecular weight markers. How accurate is it to end-label DNA markers with
>polynucleotide kinase and 32P-ATP and use those? Do I heat them first to
>denature them? Are there better methods? Thanks in advance for your help!
What we do is use cold pre-stained markers, and prior to exposing them to
the film, we dot them with a fountain pen containing ink and tailings
from old 32P tubes. Makes for a radioactive ink, which we cover with
Scotch tape (to prevent smudging on the film).
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