RNA isolation from small # of cells?

szcooley at chip.ucdavis.edu szcooley at chip.ucdavis.edu
Thu Sep 1 14:52:13 EST 1994


bormann at urz.unibas.ch wrote:
: Hi Bionetters,

: I would like to isolate RNA from a small number of cells
: (approx. 500) to finally make a library.
: Does anybody have a suggestion or experience how to do
: this?   Thanks a lot.

: Peter.

Eric Karr at CEPRAP, Univ. of Calif., Davis, CA 95616, has been trying 
for some time to do it from a single cell using a combination of T7 and 
PCR. It's very problematic.


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