zymograms for detecting lipase activity

Fri Sep 2 17:18:32 EST 1994

Hello Netters,  I am interested in an overlay technique for polyacrylamide
gels (native in this case) in which I can visualize lipase activity.  I have
a method for detecting phospholipases where the phospholipid in the overlay
is processed from an insoluble form to a soluble form  thereby creating a
clearing in the overlay.  Does a similar overlay method exist for general
lipase activity?    Thanks in advance for any replies.

Jan DeJarnette
Dept. of Microbiology
University of Maryland

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