MW Markers for RNA gels...
DRHOADS at MERCURY.UARK.EDU
Fri Sep 2 07:37:34 EST 1994
> To: methods-and-reagents at net.bio.net
> From: u8908594 at muss.cis.McMaster.CA (M.J. Principe)
> Subject: Re: MW Markers for RNA gels...
> Date: 1 Sep 1994 17:01:08 -0400
> In article <00983D38.375E2780.230 at umbsky.cc.umb.edu>,
> SPIKE <5978jsnyd at UMBSKY.CC.UMB.EDU> wrote:
> >I will be running in vitro transcripts out on polyacrylamide gels and need to
> >use molecular weight markers. How accurate is it to end-label DNA markers with
> >polynucleotide kinase and 32P-ATP and use those? Do I heat them first to
> >denature them? Are there better methods? Thanks in advance for your help!
> What we do is use cold pre-stained markers, and prior to exposing them to
> the film, we dot them with a fountain pen containing ink and tailings
> from old 32P tubes. Makes for a radioactive ink, which we cover with
> Scotch tape (to prevent smudging on the film).
Rather tan make up 32P ink you may want to try going to your local
Artists supply store. Pick up a tube of glow-in-the-dark glue such
as is sold for use on Sweatshirts. This glue has glitter and a
yellow tint to it. You can spot the stuff on plastic, dried gels,
paper, etc. A few minutes to dry in the light and then load on film.
The glow is a nice low-level green and just right for giving a nice
black spot on the film. The stuff is cheap (about $1.50), readily available and
Doug Rhoads || Dept. of Biological Sciences
drhoads at mercury.uark.edu || 601 Science Engineering
drhoads at uafsysb.uark.edu || University of Arkansas
501-575-3251 || Fayetteville, AR 72701
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