vancomycin and Agrobacterium

Andreas Weber aweber at biolan.uni-koeln.de
Thu Sep 1 06:34:50 EST 1994


In article <CvF6DI.AyK at ucdavis.edu>, szcooley at chip.ucdavis.edu () says:
>
>Bjorn Orvar (borvar at unixg.ubc.ca) wrote:
>: I am transforming tobacco leaf discs  with the agrobacterium strain LBA4404 
>: and I would like to know if I can use vancomycin for killing this strain 
>: during the selection of transformants,  instead of carbenicillin (I have a lot 
>: of vancomycin!).
>: Thank you
>: Bjorn Orvar
>
>I talked with Brad Hall. He has used vancomycin with GV3111SE but not 
>with LBA4404. It was effective at 100 ug/ml.
>
>
>#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%
>%                                                        #
>#   Dr. Michael Cooley         Practice Random Kindness  %
>%   mbcooley at ucdavis.edu    and Senseless Acts of Beauty #
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>%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#%#
>

In Plant Molecular Biology Manual B6: 1-9 (1994) Mathis and Hinchee
suggest to use vancomycin 150 mg/l when Agrobacteria still
continue to grow at 100 mg/l cefotaxim or 500 mg/l carbenicillin.


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