Spontaneous Spectinomycin resistance ?
jarnie at utu.fi
Fri Sep 2 01:59:19 EST 1994
the End (jgraham at bronze.ucs.indiana.edu) wrote:
> I am seeing a relatively high rate of acquisition of spectinomycin
> resistance in the E. coli strain I am working with (CSH20 from Miller's
> old kit). It appears that all cells in a cuture are somewhat resistant,
> and that 60 or so colonies will form from approximately 100 ul of
> competent cells spread on a plate.
> Anyone else see this ? Any relevant knowledge of mechanisms ?
> Thanks much,
> E-mail copies of all posts,
> J. E. Graham
> Biology and Chemistry Departments
> Indiana University Bloomington
Jarmo Niemi,Biochemistry,U.of Turku,jarnie at utu.fi,http://www.utu.fi/~jarnie/
"Rommel, you old fox, I_read_your_book_!"
More information about the Methods