Subcloning PCR

Guilherme Co. Oliveira guilher at
Fri Sep 2 13:36:13 EST 1994

	As someone already mentioned TA vectors are GREAT.  It has worked for
me since the first try.
	Something elase I may suggest is that you run a SDS-PAGE gel to see
if your fragment is really being digested.  I have done this and could see a
clear difference in size of digested and undigested PCR fragment.
	Good luck,
	Gui Oliveira

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