Fusion protein was trapped in the cell pellet

S.A. Benson Lab SABLAB at MICROB.UMD.EDU
Fri Sep 2 11:32:42 EST 1994


Hello, guys!
  I am using  the New England Biolab pMal p2 vector to express a 
fusion protein.  The protein was expressed, however, it was trapped 
in the cell pellet after sonication and centrifugation.  In other 
word, it might be trapped by the membrane fragments.  Would anyone 
who knows the solution gives me some help please?



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