microbial activity in soil

Dick Pratt PQQ at psuvm.psu.edu
Mon Sep 5 10:48:53 EST 1994


I would suggest fluorescein diacetate (FDA) which will show esterase
activity.  I have used this both for epifluorescent detection of
"viable" microbes and for extraction of the fluorescein produced by
esterase cleave of the FDA acetates.  This method shows about the
same level of activity/viability as INT methods and is not so sensitive
to electron acceptors.

J.R. (Dick) Pratt            pqq at psuvm.psu.edu (internet)
2B Ferguson Bldg.            FAX: 814/865-3725
Penn State                   Voice: 814/863-2095
University Park, PA 16802



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