R.HANEMAAIJER at pg.TNO.nl
Tue Sep 6 09:11:59 EST 1994
Does anybody know how to improve the efficiency of ligation?
We make cDNA libraries of immunoglobulin light and heavy chain cDNAs,
and one of the steps with a (very) low efficiency is the ligation
We use the GIBCO-BRL ligase and buffermix. The ligation is with
sticky ends, and is carried out at 15 degrees Celcius overnight. The
mixture is then precipitated with ethanol and transformation is
carried out by electroporation.
R.hanemaaijer at pg.tno.nl
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